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Temperature during early development has long-term effects on microRNA expression in Atlantic cod.

Bizuayehu TT, Johansen SD, Puvanendran V, Toften H, Babiak I - BMC Genomics (2015)

Bottom Line: The effect of temperature on methylation status of selected miRNA promoter regions was mostly inconclusive.Temperature elevation by several degrees during embryonic and larval developmental stages significantly alters the miRNA profile, both short-term and long-term.Our results suggest that a further rise in seas temperature might affect life history of Atlantic cod.

View Article: PubMed Central - PubMed

Affiliation: University of Nordland, Faculty of Biosciences and Aquaculture, Post Box 1490, 8049, Bodø, Norway. ttb@uin.no.

ABSTRACT

Background: Environmental temperature has serious implications in life cycle of aquatic ectotherms. Understanding the molecular mechanisms of temperature acclimation and adaptation of marine organisms is of the uttermost importance for ecology, fisheries, and aquaculture, as it allows modeling the effects of global warming on population dynamics. Regulatory molecules are major modulators of acclimation and adaptation; among them, microRNAs (miRNAs) are versatile and substantial contributors to regulatory networks of development and adaptive plasticity. However, their role in thermal plasticity is poorly known. We have asked whether the temperature and its shift during the early ontogeny (embryonic and larval development) affect the miRNA repertoire of Atlantic cod (Gadus morhua), and if thermal experience has long-term consequences in the miRNA profile.

Results: We characterized miRNA during different developmental stages and in juvenile tissues using next generation sequencing. We identified 389 putative miRNA precursor loci, 120 novel precursor miRNAs, and 281 mature miRNAs. Some miRNAs showed stage- or tissue-enriched expression and miRNAs, such as the miR-17 ~ 92 cluster, myomiRs (miR-206), neuromiRs (miR-9, miR-124), miR-130b, and miR-430 showed differential expression in different temperature regimes. Long-term effect of embryonic incubation temperature was revealed on expression of some miRNAs in juvenile pituitary (miR-449), gonad (miR-27c, miR-30c, and miR-200a), and liver (let-7 h, miR-7a, miR-22, miR-34c, miR-132a, miR-192, miR-221, miR-451, miR-2188, and miR-7550), but not in brain. Some of differentially expressed miRNAs in the liver were confirmed using LNA-based rt-qPCR. The effect of temperature on methylation status of selected miRNA promoter regions was mostly inconclusive.

Conclusions: Temperature elevation by several degrees during embryonic and larval developmental stages significantly alters the miRNA profile, both short-term and long-term. Our results suggest that a further rise in seas temperature might affect life history of Atlantic cod.

No MeSH data available.


Related in: MedlinePlus

Number of differentially expressed miRNAs between Atlantic cod tissues. Pairwise comparison between different tissues using NOISeq with probability of differential expression A) q > 0.95 and B) q > 0.99.
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Fig2: Number of differentially expressed miRNAs between Atlantic cod tissues. Pairwise comparison between different tissues using NOISeq with probability of differential expression A) q > 0.95 and B) q > 0.99.

Mentions: Although the diversity of miRNAs among tissues was comparable, their expression patterns were subdivided into 4 dendrograms in a tissue-specific manner regardless of temperature regimes (Additional file 4). Number of differentially expressed miRNAs varied among tissues (Figure 2). Some miRNAs were specifically abundant in a given tissue type; for example, miR-9, miR-124a, and miR-128 in the brain; miR-375 and miR-7a in the pituitary; miR-202 in gonads; and miR-122 in the liver (Additional files 4 and 5).Figure 2


Temperature during early development has long-term effects on microRNA expression in Atlantic cod.

Bizuayehu TT, Johansen SD, Puvanendran V, Toften H, Babiak I - BMC Genomics (2015)

Number of differentially expressed miRNAs between Atlantic cod tissues. Pairwise comparison between different tissues using NOISeq with probability of differential expression A) q > 0.95 and B) q > 0.99.
© Copyright Policy - open-access
Related In: Results  -  Collection

License 1 - License 2
Show All Figures
getmorefigures.php?uid=PMC4403832&req=5

Fig2: Number of differentially expressed miRNAs between Atlantic cod tissues. Pairwise comparison between different tissues using NOISeq with probability of differential expression A) q > 0.95 and B) q > 0.99.
Mentions: Although the diversity of miRNAs among tissues was comparable, their expression patterns were subdivided into 4 dendrograms in a tissue-specific manner regardless of temperature regimes (Additional file 4). Number of differentially expressed miRNAs varied among tissues (Figure 2). Some miRNAs were specifically abundant in a given tissue type; for example, miR-9, miR-124a, and miR-128 in the brain; miR-375 and miR-7a in the pituitary; miR-202 in gonads; and miR-122 in the liver (Additional files 4 and 5).Figure 2

Bottom Line: The effect of temperature on methylation status of selected miRNA promoter regions was mostly inconclusive.Temperature elevation by several degrees during embryonic and larval developmental stages significantly alters the miRNA profile, both short-term and long-term.Our results suggest that a further rise in seas temperature might affect life history of Atlantic cod.

View Article: PubMed Central - PubMed

Affiliation: University of Nordland, Faculty of Biosciences and Aquaculture, Post Box 1490, 8049, Bodø, Norway. ttb@uin.no.

ABSTRACT

Background: Environmental temperature has serious implications in life cycle of aquatic ectotherms. Understanding the molecular mechanisms of temperature acclimation and adaptation of marine organisms is of the uttermost importance for ecology, fisheries, and aquaculture, as it allows modeling the effects of global warming on population dynamics. Regulatory molecules are major modulators of acclimation and adaptation; among them, microRNAs (miRNAs) are versatile and substantial contributors to regulatory networks of development and adaptive plasticity. However, their role in thermal plasticity is poorly known. We have asked whether the temperature and its shift during the early ontogeny (embryonic and larval development) affect the miRNA repertoire of Atlantic cod (Gadus morhua), and if thermal experience has long-term consequences in the miRNA profile.

Results: We characterized miRNA during different developmental stages and in juvenile tissues using next generation sequencing. We identified 389 putative miRNA precursor loci, 120 novel precursor miRNAs, and 281 mature miRNAs. Some miRNAs showed stage- or tissue-enriched expression and miRNAs, such as the miR-17 ~ 92 cluster, myomiRs (miR-206), neuromiRs (miR-9, miR-124), miR-130b, and miR-430 showed differential expression in different temperature regimes. Long-term effect of embryonic incubation temperature was revealed on expression of some miRNAs in juvenile pituitary (miR-449), gonad (miR-27c, miR-30c, and miR-200a), and liver (let-7 h, miR-7a, miR-22, miR-34c, miR-132a, miR-192, miR-221, miR-451, miR-2188, and miR-7550), but not in brain. Some of differentially expressed miRNAs in the liver were confirmed using LNA-based rt-qPCR. The effect of temperature on methylation status of selected miRNA promoter regions was mostly inconclusive.

Conclusions: Temperature elevation by several degrees during embryonic and larval developmental stages significantly alters the miRNA profile, both short-term and long-term. Our results suggest that a further rise in seas temperature might affect life history of Atlantic cod.

No MeSH data available.


Related in: MedlinePlus