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The effects of different tensile parameters for the neurodynamic mobilization technique on tricipital muscle wet weight and MuRf-1 expression in rabbits with sciatic nerve injury.

Wang Y, Ma M, Tang Q, Zhu L, Koleini M, Zou D - J Neuroeng Rehabil (2015)

Bottom Line: Six adult rabbits were measured to determine the relationship between the joint angle of the lower limb and percent of sciatic nerve elongation to define the tensile parameters of NMT; Thirty adult rabbits were randomly assigned into a sham, model, NMT-A, NMT-B, or NMT-C groups.In addition, MuRf-1 expression was significantly reduced in the NMT-B group compared with the NMT-A, NMT-C, and model groups.Elongating the nerve by NMT of 9% in rabbits decreased MuRf-1 expression and decelerated muscle atrophy in the subjects with sciatic nerve injury.

View Article: PubMed Central - PubMed

Affiliation: Rehabilitation medicine center of the second affiliated hospital of Heilongjiang university of Chinese medicine, Harbin, 150001, China. swallow-1113@163.com.

ABSTRACT

Background: After peripheral nerve injury, muscles without innervation begin to undergo atrophy. Research has suggested that MuRf-1 may play a role in muscle atrophy. The neurodynamic mobilization technique (NMT) is a manual therapy method used to elongate a nerve along its long axis, resulting in improved blood flow to the nerve. However, the nerve can be damaged if elongated too much. The purpose of this study is to observe the effect of NMT on muscle wet weight and MuRf-1 expression in rabbits with sciatic nerve injury.

Methods: Six adult rabbits were measured to determine the relationship between the joint angle of the lower limb and percent of sciatic nerve elongation to define the tensile parameters of NMT; Thirty adult rabbits were randomly assigned into a sham, model, NMT-A, NMT-B, or NMT-C groups. Four weeks post-treatment, the wet mass of the tricipital muscles and MuRf-1 expression were observed.

Results: The wet mass of the tricipital muscles in the NMT-B group was significantly greater than the NMT-A, NMT-C, and model groups. In addition, MuRf-1 expression was significantly reduced in the NMT-B group compared with the NMT-A, NMT-C, and model groups.

Conclusions: Elongating the nerve by NMT of 9% in rabbits decreased MuRf-1 expression and decelerated muscle atrophy in the subjects with sciatic nerve injury.

No MeSH data available.


Related in: MedlinePlus

Original length of the sciatic nerve (mm).
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Fig1: Original length of the sciatic nerve (mm).

Mentions: Six 4 ~ 5-months old male adult Japanese white rabbits with a mean weight of 1.79 kg were supplied by Harbin Pacific Biological Pharmaceutical Co., LTD. [license SCXK (Hei), 2011–009]. After randomly numbering the rabbits from 1 to 6, they were anaesthetized with 1–2 ml/kg of 10% chloral hydrate via the ear marginal vein. A lateral approach was created, the attachment point of rectus femoris tendon on knee joint and the rectus femoris between up one third and middle were cut, and the sciatic nerve was exposed fully. Next, two surgical sutures, one was on 1 cm above lateral femoral epicondyle, another was on going up 20 mm apart, were fastened to the nerve trunk as markers. The rabbits were placed on the experiment table with their hip and knee joints at 45° and 30° angles, respectively. The knee joints of the rabbits were passively extended along the sciatic nerve. Meanwhile, Digital Vernier Calipers were placed on the nerve trunk with a precision of 0.01 mm. When the distance between the two markers in the nerve trunk was elongated to the appropriate length (nerve elongation 6%, 9% and 12% is 21.2 mm, 21.8 mm and 22.4 mm, respectively), the angle of the knee joint was measured. The mean of the knee joint angles of the six rabbits was used as the standard of NMT for different nerve elongations (Figures 1, 2, and 3). The relationship between the lower limb joint angle and sciatic nerve elongation is described as Table 1.Figure 1


The effects of different tensile parameters for the neurodynamic mobilization technique on tricipital muscle wet weight and MuRf-1 expression in rabbits with sciatic nerve injury.

Wang Y, Ma M, Tang Q, Zhu L, Koleini M, Zou D - J Neuroeng Rehabil (2015)

Original length of the sciatic nerve (mm).
© Copyright Policy - open-access
Related In: Results  -  Collection

License 1 - License 2
Show All Figures
getmorefigures.php?uid=PMC4403708&req=5

Fig1: Original length of the sciatic nerve (mm).
Mentions: Six 4 ~ 5-months old male adult Japanese white rabbits with a mean weight of 1.79 kg were supplied by Harbin Pacific Biological Pharmaceutical Co., LTD. [license SCXK (Hei), 2011–009]. After randomly numbering the rabbits from 1 to 6, they were anaesthetized with 1–2 ml/kg of 10% chloral hydrate via the ear marginal vein. A lateral approach was created, the attachment point of rectus femoris tendon on knee joint and the rectus femoris between up one third and middle were cut, and the sciatic nerve was exposed fully. Next, two surgical sutures, one was on 1 cm above lateral femoral epicondyle, another was on going up 20 mm apart, were fastened to the nerve trunk as markers. The rabbits were placed on the experiment table with their hip and knee joints at 45° and 30° angles, respectively. The knee joints of the rabbits were passively extended along the sciatic nerve. Meanwhile, Digital Vernier Calipers were placed on the nerve trunk with a precision of 0.01 mm. When the distance between the two markers in the nerve trunk was elongated to the appropriate length (nerve elongation 6%, 9% and 12% is 21.2 mm, 21.8 mm and 22.4 mm, respectively), the angle of the knee joint was measured. The mean of the knee joint angles of the six rabbits was used as the standard of NMT for different nerve elongations (Figures 1, 2, and 3). The relationship between the lower limb joint angle and sciatic nerve elongation is described as Table 1.Figure 1

Bottom Line: Six adult rabbits were measured to determine the relationship between the joint angle of the lower limb and percent of sciatic nerve elongation to define the tensile parameters of NMT; Thirty adult rabbits were randomly assigned into a sham, model, NMT-A, NMT-B, or NMT-C groups.In addition, MuRf-1 expression was significantly reduced in the NMT-B group compared with the NMT-A, NMT-C, and model groups.Elongating the nerve by NMT of 9% in rabbits decreased MuRf-1 expression and decelerated muscle atrophy in the subjects with sciatic nerve injury.

View Article: PubMed Central - PubMed

Affiliation: Rehabilitation medicine center of the second affiliated hospital of Heilongjiang university of Chinese medicine, Harbin, 150001, China. swallow-1113@163.com.

ABSTRACT

Background: After peripheral nerve injury, muscles without innervation begin to undergo atrophy. Research has suggested that MuRf-1 may play a role in muscle atrophy. The neurodynamic mobilization technique (NMT) is a manual therapy method used to elongate a nerve along its long axis, resulting in improved blood flow to the nerve. However, the nerve can be damaged if elongated too much. The purpose of this study is to observe the effect of NMT on muscle wet weight and MuRf-1 expression in rabbits with sciatic nerve injury.

Methods: Six adult rabbits were measured to determine the relationship between the joint angle of the lower limb and percent of sciatic nerve elongation to define the tensile parameters of NMT; Thirty adult rabbits were randomly assigned into a sham, model, NMT-A, NMT-B, or NMT-C groups. Four weeks post-treatment, the wet mass of the tricipital muscles and MuRf-1 expression were observed.

Results: The wet mass of the tricipital muscles in the NMT-B group was significantly greater than the NMT-A, NMT-C, and model groups. In addition, MuRf-1 expression was significantly reduced in the NMT-B group compared with the NMT-A, NMT-C, and model groups.

Conclusions: Elongating the nerve by NMT of 9% in rabbits decreased MuRf-1 expression and decelerated muscle atrophy in the subjects with sciatic nerve injury.

No MeSH data available.


Related in: MedlinePlus