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Synthesis, characterization, and immune efficacy of layered double hydroxide@SiO2 nanoparticles with shell-core structure as a delivery carrier for Newcastle disease virus DNA vaccine.

Zhao K, Rong G, Guo C, Luo X, Kang H, Sun Y, Dai C, Wang X, Wang X, Jin Z, Cui S, Sun Q - Int J Nanomedicine (2015)

Bottom Line: The results demonstrated that the pFDNA-LDH@SiO2-NPs had a regular morphology and high stability with a mean diameter of 371.93 nm, loading capacity of 39.66%±0.45%, and a zeta potential of +31.63 mV.Additionally, their high transfection efficiency in vitro was detected by fluorescent microscopy.Intranasal immunization of specific pathogen-free chickens with pFDNA-LDH@SiO2-NPs induced stronger cellular, humoral, and mucosal immune responses and achieved a greater sustained release effect than intramuscular naked plasmid DNA, and the protective efficacy after challenge with the strain F48E9 with highly virulent (mean death time of chicken embryos ≤60 hours, intracerebral pathogenicity index in 1 -day-old chickens >1.6) was 100%.

View Article: PubMed Central - PubMed

Affiliation: Key Laboratory of Microbiology, School of Life Science, Heilongjiang University, Harbin, People's Republic of China.

ABSTRACT
Layered double hydroxide (LDH)@SiO2 nanoparticles were developed as a delivery carrier for the plasmid DNA expressing the Newcastle disease virus F gene. The LDH was hydrotalcite-like materials. The plasmid DNA encapsulated in the LDH@SiO2 nanoparticles (pFDNA-LDH@SiO2-NPs) was prepared by the coprecipitation method, and the properties of pFDNA-LDH@SiO2-NPs were characterized by transmission electron microscopy, zeta potential analyzer, Fourier transform infrared spectroscopy, and X-ray diffraction analysis. The results demonstrated that the pFDNA-LDH@SiO2-NPs had a regular morphology and high stability with a mean diameter of 371.93 nm, loading capacity of 39.66%±0.45%, and a zeta potential of +31.63 mV. A release assay in vitro showed that up to 91.36% of the total plasmid DNA could be sustainably released from the pFDNA-LDH@SiO2-NPs within 288 hours. The LDH@SiO2 nanoparticles had very low toxicity. Additionally, their high transfection efficiency in vitro was detected by fluorescent microscopy. Intranasal immunization of specific pathogen-free chickens with pFDNA-LDH@SiO2-NPs induced stronger cellular, humoral, and mucosal immune responses and achieved a greater sustained release effect than intramuscular naked plasmid DNA, and the protective efficacy after challenge with the strain F48E9 with highly virulent (mean death time of chicken embryos ≤60 hours, intracerebral pathogenicity index in 1 -day-old chickens >1.6) was 100%. Based on the results, LDH@SiO2 nanoparticles can be used as a delivery carrier for mucosal immunity of Newcastle disease DNA vaccine, and have great application potential in the future.

No MeSH data available.


Related in: MedlinePlus

IgA antibody levels in serum (A), tracheal fluid (B), bile (C), and Harderian glands (D) of specific pathogen-free chickens immunized with PBS IM, blank LDH@SiO2-NPs IM, naked plasmid pVAX1-F(o) DNA IM, pFDNA-LDH@SiO2-NPs IM, and pFDNA-LDH@SiO2-NPs IN.Notes: IgA antibody levels are presented as mean ± standard deviation of five experiments. Data with different small letters show significant difference (P<0.05).Abbreviations: IM, intramuscularly; IN, intranasally; LDH, layered double hydroxide; NPs, nanoparticles; PBS, phosphate-buffered saline; pFDNA-LDH@SiO2-NPs, Newcastle disease virus F gene encapsulated in the LDH@SiO2-NPs.
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f9-ijn-10-2895: IgA antibody levels in serum (A), tracheal fluid (B), bile (C), and Harderian glands (D) of specific pathogen-free chickens immunized with PBS IM, blank LDH@SiO2-NPs IM, naked plasmid pVAX1-F(o) DNA IM, pFDNA-LDH@SiO2-NPs IM, and pFDNA-LDH@SiO2-NPs IN.Notes: IgA antibody levels are presented as mean ± standard deviation of five experiments. Data with different small letters show significant difference (P<0.05).Abbreviations: IM, intramuscularly; IN, intranasally; LDH, layered double hydroxide; NPs, nanoparticles; PBS, phosphate-buffered saline; pFDNA-LDH@SiO2-NPs, Newcastle disease virus F gene encapsulated in the LDH@SiO2-NPs.

Mentions: The changes of IgA antibody levels in serum, bile, tracheal fluid, and Harderian glands post-immunization are shown in Figure 9. The IgA antibody levels in the pFDNA-LDH@SiO2-NPs IN, pFDNA-LDH@SiO2-NPs IM and naked plasmid DNA IM groups were significantly higher than those in the blank LDH@SiO2-NPs IM and PBS IM groups (P<0.01). The chickens immunized with the pFDNA-LDH@SiO2-NPs IN had significantly higher IgA antibody titers (P<0.01) and longer period of IgA antibody secretion in serum (Figure 9A), tracheal fluid (Figure 9B), bile (Figure 9C), and Harderian glands (Figure 9D) than the chickens of other groups. The IgA antibody titers in the pFDNA-LDH@SiO2-NPs IM group were significantly higher and their secretion period longer than in the naked plasmid DNA IM group (P<0.05). It can be clearly observed that pFDNA-LDH@SiO2 -NPs were able to induce quicker and better mucosal immune responses than the naked plasmid DNA vaccine.


Synthesis, characterization, and immune efficacy of layered double hydroxide@SiO2 nanoparticles with shell-core structure as a delivery carrier for Newcastle disease virus DNA vaccine.

Zhao K, Rong G, Guo C, Luo X, Kang H, Sun Y, Dai C, Wang X, Wang X, Jin Z, Cui S, Sun Q - Int J Nanomedicine (2015)

IgA antibody levels in serum (A), tracheal fluid (B), bile (C), and Harderian glands (D) of specific pathogen-free chickens immunized with PBS IM, blank LDH@SiO2-NPs IM, naked plasmid pVAX1-F(o) DNA IM, pFDNA-LDH@SiO2-NPs IM, and pFDNA-LDH@SiO2-NPs IN.Notes: IgA antibody levels are presented as mean ± standard deviation of five experiments. Data with different small letters show significant difference (P<0.05).Abbreviations: IM, intramuscularly; IN, intranasally; LDH, layered double hydroxide; NPs, nanoparticles; PBS, phosphate-buffered saline; pFDNA-LDH@SiO2-NPs, Newcastle disease virus F gene encapsulated in the LDH@SiO2-NPs.
© Copyright Policy
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4403701&req=5

f9-ijn-10-2895: IgA antibody levels in serum (A), tracheal fluid (B), bile (C), and Harderian glands (D) of specific pathogen-free chickens immunized with PBS IM, blank LDH@SiO2-NPs IM, naked plasmid pVAX1-F(o) DNA IM, pFDNA-LDH@SiO2-NPs IM, and pFDNA-LDH@SiO2-NPs IN.Notes: IgA antibody levels are presented as mean ± standard deviation of five experiments. Data with different small letters show significant difference (P<0.05).Abbreviations: IM, intramuscularly; IN, intranasally; LDH, layered double hydroxide; NPs, nanoparticles; PBS, phosphate-buffered saline; pFDNA-LDH@SiO2-NPs, Newcastle disease virus F gene encapsulated in the LDH@SiO2-NPs.
Mentions: The changes of IgA antibody levels in serum, bile, tracheal fluid, and Harderian glands post-immunization are shown in Figure 9. The IgA antibody levels in the pFDNA-LDH@SiO2-NPs IN, pFDNA-LDH@SiO2-NPs IM and naked plasmid DNA IM groups were significantly higher than those in the blank LDH@SiO2-NPs IM and PBS IM groups (P<0.01). The chickens immunized with the pFDNA-LDH@SiO2-NPs IN had significantly higher IgA antibody titers (P<0.01) and longer period of IgA antibody secretion in serum (Figure 9A), tracheal fluid (Figure 9B), bile (Figure 9C), and Harderian glands (Figure 9D) than the chickens of other groups. The IgA antibody titers in the pFDNA-LDH@SiO2-NPs IM group were significantly higher and their secretion period longer than in the naked plasmid DNA IM group (P<0.05). It can be clearly observed that pFDNA-LDH@SiO2 -NPs were able to induce quicker and better mucosal immune responses than the naked plasmid DNA vaccine.

Bottom Line: The results demonstrated that the pFDNA-LDH@SiO2-NPs had a regular morphology and high stability with a mean diameter of 371.93 nm, loading capacity of 39.66%±0.45%, and a zeta potential of +31.63 mV.Additionally, their high transfection efficiency in vitro was detected by fluorescent microscopy.Intranasal immunization of specific pathogen-free chickens with pFDNA-LDH@SiO2-NPs induced stronger cellular, humoral, and mucosal immune responses and achieved a greater sustained release effect than intramuscular naked plasmid DNA, and the protective efficacy after challenge with the strain F48E9 with highly virulent (mean death time of chicken embryos ≤60 hours, intracerebral pathogenicity index in 1 -day-old chickens >1.6) was 100%.

View Article: PubMed Central - PubMed

Affiliation: Key Laboratory of Microbiology, School of Life Science, Heilongjiang University, Harbin, People's Republic of China.

ABSTRACT
Layered double hydroxide (LDH)@SiO2 nanoparticles were developed as a delivery carrier for the plasmid DNA expressing the Newcastle disease virus F gene. The LDH was hydrotalcite-like materials. The plasmid DNA encapsulated in the LDH@SiO2 nanoparticles (pFDNA-LDH@SiO2-NPs) was prepared by the coprecipitation method, and the properties of pFDNA-LDH@SiO2-NPs were characterized by transmission electron microscopy, zeta potential analyzer, Fourier transform infrared spectroscopy, and X-ray diffraction analysis. The results demonstrated that the pFDNA-LDH@SiO2-NPs had a regular morphology and high stability with a mean diameter of 371.93 nm, loading capacity of 39.66%±0.45%, and a zeta potential of +31.63 mV. A release assay in vitro showed that up to 91.36% of the total plasmid DNA could be sustainably released from the pFDNA-LDH@SiO2-NPs within 288 hours. The LDH@SiO2 nanoparticles had very low toxicity. Additionally, their high transfection efficiency in vitro was detected by fluorescent microscopy. Intranasal immunization of specific pathogen-free chickens with pFDNA-LDH@SiO2-NPs induced stronger cellular, humoral, and mucosal immune responses and achieved a greater sustained release effect than intramuscular naked plasmid DNA, and the protective efficacy after challenge with the strain F48E9 with highly virulent (mean death time of chicken embryos ≤60 hours, intracerebral pathogenicity index in 1 -day-old chickens >1.6) was 100%. Based on the results, LDH@SiO2 nanoparticles can be used as a delivery carrier for mucosal immunity of Newcastle disease DNA vaccine, and have great application potential in the future.

No MeSH data available.


Related in: MedlinePlus