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Synthesis, characterization, and immune efficacy of layered double hydroxide@SiO2 nanoparticles with shell-core structure as a delivery carrier for Newcastle disease virus DNA vaccine.

Zhao K, Rong G, Guo C, Luo X, Kang H, Sun Y, Dai C, Wang X, Wang X, Jin Z, Cui S, Sun Q - Int J Nanomedicine (2015)

Bottom Line: The results demonstrated that the pFDNA-LDH@SiO2-NPs had a regular morphology and high stability with a mean diameter of 371.93 nm, loading capacity of 39.66%±0.45%, and a zeta potential of +31.63 mV.Additionally, their high transfection efficiency in vitro was detected by fluorescent microscopy.Intranasal immunization of specific pathogen-free chickens with pFDNA-LDH@SiO2-NPs induced stronger cellular, humoral, and mucosal immune responses and achieved a greater sustained release effect than intramuscular naked plasmid DNA, and the protective efficacy after challenge with the strain F48E9 with highly virulent (mean death time of chicken embryos ≤60 hours, intracerebral pathogenicity index in 1 -day-old chickens >1.6) was 100%.

View Article: PubMed Central - PubMed

Affiliation: Key Laboratory of Microbiology, School of Life Science, Heilongjiang University, Harbin, People's Republic of China.

ABSTRACT
Layered double hydroxide (LDH)@SiO2 nanoparticles were developed as a delivery carrier for the plasmid DNA expressing the Newcastle disease virus F gene. The LDH was hydrotalcite-like materials. The plasmid DNA encapsulated in the LDH@SiO2 nanoparticles (pFDNA-LDH@SiO2-NPs) was prepared by the coprecipitation method, and the properties of pFDNA-LDH@SiO2-NPs were characterized by transmission electron microscopy, zeta potential analyzer, Fourier transform infrared spectroscopy, and X-ray diffraction analysis. The results demonstrated that the pFDNA-LDH@SiO2-NPs had a regular morphology and high stability with a mean diameter of 371.93 nm, loading capacity of 39.66%±0.45%, and a zeta potential of +31.63 mV. A release assay in vitro showed that up to 91.36% of the total plasmid DNA could be sustainably released from the pFDNA-LDH@SiO2-NPs within 288 hours. The LDH@SiO2 nanoparticles had very low toxicity. Additionally, their high transfection efficiency in vitro was detected by fluorescent microscopy. Intranasal immunization of specific pathogen-free chickens with pFDNA-LDH@SiO2-NPs induced stronger cellular, humoral, and mucosal immune responses and achieved a greater sustained release effect than intramuscular naked plasmid DNA, and the protective efficacy after challenge with the strain F48E9 with highly virulent (mean death time of chicken embryos ≤60 hours, intracerebral pathogenicity index in 1 -day-old chickens >1.6) was 100%. Based on the results, LDH@SiO2 nanoparticles can be used as a delivery carrier for mucosal immunity of Newcastle disease DNA vaccine, and have great application potential in the future.

No MeSH data available.


Related in: MedlinePlus

Histopathological analyses of normal duodena, proventriculi, and myocardia.Notes: (A) Tissues of duodena were immunized with pFDNA-LDH@SiO2-NPs IM (a), pFDNA-LDH@SiO2-NPs IN (b), and PBS IM (c). (B) Tissues of proventriculi were immunized with pFDNA-LDH@SiO2-NPs IM (a), pFDNA-LDH@SiO2-NPs IN (b), and PBS IM (c). (C) Tissues of myocardia were immunized with pFDNA-LDH@SiO2-NPs IM (a), pFDNA-LDH@SiO2-NPs IN (b), and PBS IM (c).Abbreviations: IM, intramuscularly; IN, intranasally; LDH, layered double hydroxide; NPs, nanoparticles; PBS, phosphate-buffered saline; pFDNA-LDH@SiO2-NPs, Newcastle disease virus F gene encapsulated in LDH@SiO2-NPs.
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f8-ijn-10-2895: Histopathological analyses of normal duodena, proventriculi, and myocardia.Notes: (A) Tissues of duodena were immunized with pFDNA-LDH@SiO2-NPs IM (a), pFDNA-LDH@SiO2-NPs IN (b), and PBS IM (c). (B) Tissues of proventriculi were immunized with pFDNA-LDH@SiO2-NPs IM (a), pFDNA-LDH@SiO2-NPs IN (b), and PBS IM (c). (C) Tissues of myocardia were immunized with pFDNA-LDH@SiO2-NPs IM (a), pFDNA-LDH@SiO2-NPs IN (b), and PBS IM (c).Abbreviations: IM, intramuscularly; IN, intranasally; LDH, layered double hydroxide; NPs, nanoparticles; PBS, phosphate-buffered saline; pFDNA-LDH@SiO2-NPs, Newcastle disease virus F gene encapsulated in LDH@SiO2-NPs.

Mentions: Compared with those in the control group, the feeding, water intake, and other behaviors were normal in the chickens immunized IM and IN with the pFDNA-LDH@SiO2-NPs, implying that immunization of the chickens with a high dose of pFDNA-LDH@SiO2-NPs is safe. The chickens from the three groups were euthanized, and their duodena, proventriculi, and myocardia were then taken out for pathological section analysis. The results are shown in Figure 8.


Synthesis, characterization, and immune efficacy of layered double hydroxide@SiO2 nanoparticles with shell-core structure as a delivery carrier for Newcastle disease virus DNA vaccine.

Zhao K, Rong G, Guo C, Luo X, Kang H, Sun Y, Dai C, Wang X, Wang X, Jin Z, Cui S, Sun Q - Int J Nanomedicine (2015)

Histopathological analyses of normal duodena, proventriculi, and myocardia.Notes: (A) Tissues of duodena were immunized with pFDNA-LDH@SiO2-NPs IM (a), pFDNA-LDH@SiO2-NPs IN (b), and PBS IM (c). (B) Tissues of proventriculi were immunized with pFDNA-LDH@SiO2-NPs IM (a), pFDNA-LDH@SiO2-NPs IN (b), and PBS IM (c). (C) Tissues of myocardia were immunized with pFDNA-LDH@SiO2-NPs IM (a), pFDNA-LDH@SiO2-NPs IN (b), and PBS IM (c).Abbreviations: IM, intramuscularly; IN, intranasally; LDH, layered double hydroxide; NPs, nanoparticles; PBS, phosphate-buffered saline; pFDNA-LDH@SiO2-NPs, Newcastle disease virus F gene encapsulated in LDH@SiO2-NPs.
© Copyright Policy
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4403701&req=5

f8-ijn-10-2895: Histopathological analyses of normal duodena, proventriculi, and myocardia.Notes: (A) Tissues of duodena were immunized with pFDNA-LDH@SiO2-NPs IM (a), pFDNA-LDH@SiO2-NPs IN (b), and PBS IM (c). (B) Tissues of proventriculi were immunized with pFDNA-LDH@SiO2-NPs IM (a), pFDNA-LDH@SiO2-NPs IN (b), and PBS IM (c). (C) Tissues of myocardia were immunized with pFDNA-LDH@SiO2-NPs IM (a), pFDNA-LDH@SiO2-NPs IN (b), and PBS IM (c).Abbreviations: IM, intramuscularly; IN, intranasally; LDH, layered double hydroxide; NPs, nanoparticles; PBS, phosphate-buffered saline; pFDNA-LDH@SiO2-NPs, Newcastle disease virus F gene encapsulated in LDH@SiO2-NPs.
Mentions: Compared with those in the control group, the feeding, water intake, and other behaviors were normal in the chickens immunized IM and IN with the pFDNA-LDH@SiO2-NPs, implying that immunization of the chickens with a high dose of pFDNA-LDH@SiO2-NPs is safe. The chickens from the three groups were euthanized, and their duodena, proventriculi, and myocardia were then taken out for pathological section analysis. The results are shown in Figure 8.

Bottom Line: The results demonstrated that the pFDNA-LDH@SiO2-NPs had a regular morphology and high stability with a mean diameter of 371.93 nm, loading capacity of 39.66%±0.45%, and a zeta potential of +31.63 mV.Additionally, their high transfection efficiency in vitro was detected by fluorescent microscopy.Intranasal immunization of specific pathogen-free chickens with pFDNA-LDH@SiO2-NPs induced stronger cellular, humoral, and mucosal immune responses and achieved a greater sustained release effect than intramuscular naked plasmid DNA, and the protective efficacy after challenge with the strain F48E9 with highly virulent (mean death time of chicken embryos ≤60 hours, intracerebral pathogenicity index in 1 -day-old chickens >1.6) was 100%.

View Article: PubMed Central - PubMed

Affiliation: Key Laboratory of Microbiology, School of Life Science, Heilongjiang University, Harbin, People's Republic of China.

ABSTRACT
Layered double hydroxide (LDH)@SiO2 nanoparticles were developed as a delivery carrier for the plasmid DNA expressing the Newcastle disease virus F gene. The LDH was hydrotalcite-like materials. The plasmid DNA encapsulated in the LDH@SiO2 nanoparticles (pFDNA-LDH@SiO2-NPs) was prepared by the coprecipitation method, and the properties of pFDNA-LDH@SiO2-NPs were characterized by transmission electron microscopy, zeta potential analyzer, Fourier transform infrared spectroscopy, and X-ray diffraction analysis. The results demonstrated that the pFDNA-LDH@SiO2-NPs had a regular morphology and high stability with a mean diameter of 371.93 nm, loading capacity of 39.66%±0.45%, and a zeta potential of +31.63 mV. A release assay in vitro showed that up to 91.36% of the total plasmid DNA could be sustainably released from the pFDNA-LDH@SiO2-NPs within 288 hours. The LDH@SiO2 nanoparticles had very low toxicity. Additionally, their high transfection efficiency in vitro was detected by fluorescent microscopy. Intranasal immunization of specific pathogen-free chickens with pFDNA-LDH@SiO2-NPs induced stronger cellular, humoral, and mucosal immune responses and achieved a greater sustained release effect than intramuscular naked plasmid DNA, and the protective efficacy after challenge with the strain F48E9 with highly virulent (mean death time of chicken embryos ≤60 hours, intracerebral pathogenicity index in 1 -day-old chickens >1.6) was 100%. Based on the results, LDH@SiO2 nanoparticles can be used as a delivery carrier for mucosal immunity of Newcastle disease DNA vaccine, and have great application potential in the future.

No MeSH data available.


Related in: MedlinePlus