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Synthesis, characterization, and immune efficacy of layered double hydroxide@SiO2 nanoparticles with shell-core structure as a delivery carrier for Newcastle disease virus DNA vaccine.

Zhao K, Rong G, Guo C, Luo X, Kang H, Sun Y, Dai C, Wang X, Wang X, Jin Z, Cui S, Sun Q - Int J Nanomedicine (2015)

Bottom Line: The results demonstrated that the pFDNA-LDH@SiO2-NPs had a regular morphology and high stability with a mean diameter of 371.93 nm, loading capacity of 39.66%±0.45%, and a zeta potential of +31.63 mV.Additionally, their high transfection efficiency in vitro was detected by fluorescent microscopy.Intranasal immunization of specific pathogen-free chickens with pFDNA-LDH@SiO2-NPs induced stronger cellular, humoral, and mucosal immune responses and achieved a greater sustained release effect than intramuscular naked plasmid DNA, and the protective efficacy after challenge with the strain F48E9 with highly virulent (mean death time of chicken embryos ≤60 hours, intracerebral pathogenicity index in 1 -day-old chickens >1.6) was 100%.

View Article: PubMed Central - PubMed

Affiliation: Key Laboratory of Microbiology, School of Life Science, Heilongjiang University, Harbin, People's Republic of China.

ABSTRACT
Layered double hydroxide (LDH)@SiO2 nanoparticles were developed as a delivery carrier for the plasmid DNA expressing the Newcastle disease virus F gene. The LDH was hydrotalcite-like materials. The plasmid DNA encapsulated in the LDH@SiO2 nanoparticles (pFDNA-LDH@SiO2-NPs) was prepared by the coprecipitation method, and the properties of pFDNA-LDH@SiO2-NPs were characterized by transmission electron microscopy, zeta potential analyzer, Fourier transform infrared spectroscopy, and X-ray diffraction analysis. The results demonstrated that the pFDNA-LDH@SiO2-NPs had a regular morphology and high stability with a mean diameter of 371.93 nm, loading capacity of 39.66%±0.45%, and a zeta potential of +31.63 mV. A release assay in vitro showed that up to 91.36% of the total plasmid DNA could be sustainably released from the pFDNA-LDH@SiO2-NPs within 288 hours. The LDH@SiO2 nanoparticles had very low toxicity. Additionally, their high transfection efficiency in vitro was detected by fluorescent microscopy. Intranasal immunization of specific pathogen-free chickens with pFDNA-LDH@SiO2-NPs induced stronger cellular, humoral, and mucosal immune responses and achieved a greater sustained release effect than intramuscular naked plasmid DNA, and the protective efficacy after challenge with the strain F48E9 with highly virulent (mean death time of chicken embryos ≤60 hours, intracerebral pathogenicity index in 1 -day-old chickens >1.6) was 100%. Based on the results, LDH@SiO2 nanoparticles can be used as a delivery carrier for mucosal immunity of Newcastle disease DNA vaccine, and have great application potential in the future.

No MeSH data available.


Related in: MedlinePlus

In vitro expression of the pFDNA-LDH@SiO2-NPs in 293 T cells assayed by indirect immunofluorescence (×40).Notes: (A) Naked plasmid pVAX1-F(o) DNA group. (B) Transfected pFDNA-LDH@SiO2-NPs group. (C) Blank LDH@SiO2-NPs group. (D) 293 T cell group as the negative control.Abbreviations: LDH, layered double hydroxide; NPs, nanoparticles; pFDNA-LDH@SiO2-NPs, Newcastle disease virus F gene encapsulated in LDH@SiO2-NPs.
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f7-ijn-10-2895: In vitro expression of the pFDNA-LDH@SiO2-NPs in 293 T cells assayed by indirect immunofluorescence (×40).Notes: (A) Naked plasmid pVAX1-F(o) DNA group. (B) Transfected pFDNA-LDH@SiO2-NPs group. (C) Blank LDH@SiO2-NPs group. (D) 293 T cell group as the negative control.Abbreviations: LDH, layered double hydroxide; NPs, nanoparticles; pFDNA-LDH@SiO2-NPs, Newcastle disease virus F gene encapsulated in LDH@SiO2-NPs.

Mentions: The pFDNA-LDH@SiO2-NP group (Figure 7A) and the naked plasmid DNA group (Figure 7B) could be expressed in the cells and the specific fluorescence could be observed. However, no fluorescence signals were observed in the blank LDH@SiO2-NP group (Figure 7C) or the negative cell control group (Figure 7D). These results indicate that plasmid DNA can be effectively encapsulated by LDH@SiO2, and that the plasmid DNA encapsulated in pFDNA-LDH@SiO2-NPs can be expressed in vitro.


Synthesis, characterization, and immune efficacy of layered double hydroxide@SiO2 nanoparticles with shell-core structure as a delivery carrier for Newcastle disease virus DNA vaccine.

Zhao K, Rong G, Guo C, Luo X, Kang H, Sun Y, Dai C, Wang X, Wang X, Jin Z, Cui S, Sun Q - Int J Nanomedicine (2015)

In vitro expression of the pFDNA-LDH@SiO2-NPs in 293 T cells assayed by indirect immunofluorescence (×40).Notes: (A) Naked plasmid pVAX1-F(o) DNA group. (B) Transfected pFDNA-LDH@SiO2-NPs group. (C) Blank LDH@SiO2-NPs group. (D) 293 T cell group as the negative control.Abbreviations: LDH, layered double hydroxide; NPs, nanoparticles; pFDNA-LDH@SiO2-NPs, Newcastle disease virus F gene encapsulated in LDH@SiO2-NPs.
© Copyright Policy
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4403701&req=5

f7-ijn-10-2895: In vitro expression of the pFDNA-LDH@SiO2-NPs in 293 T cells assayed by indirect immunofluorescence (×40).Notes: (A) Naked plasmid pVAX1-F(o) DNA group. (B) Transfected pFDNA-LDH@SiO2-NPs group. (C) Blank LDH@SiO2-NPs group. (D) 293 T cell group as the negative control.Abbreviations: LDH, layered double hydroxide; NPs, nanoparticles; pFDNA-LDH@SiO2-NPs, Newcastle disease virus F gene encapsulated in LDH@SiO2-NPs.
Mentions: The pFDNA-LDH@SiO2-NP group (Figure 7A) and the naked plasmid DNA group (Figure 7B) could be expressed in the cells and the specific fluorescence could be observed. However, no fluorescence signals were observed in the blank LDH@SiO2-NP group (Figure 7C) or the negative cell control group (Figure 7D). These results indicate that plasmid DNA can be effectively encapsulated by LDH@SiO2, and that the plasmid DNA encapsulated in pFDNA-LDH@SiO2-NPs can be expressed in vitro.

Bottom Line: The results demonstrated that the pFDNA-LDH@SiO2-NPs had a regular morphology and high stability with a mean diameter of 371.93 nm, loading capacity of 39.66%±0.45%, and a zeta potential of +31.63 mV.Additionally, their high transfection efficiency in vitro was detected by fluorescent microscopy.Intranasal immunization of specific pathogen-free chickens with pFDNA-LDH@SiO2-NPs induced stronger cellular, humoral, and mucosal immune responses and achieved a greater sustained release effect than intramuscular naked plasmid DNA, and the protective efficacy after challenge with the strain F48E9 with highly virulent (mean death time of chicken embryos ≤60 hours, intracerebral pathogenicity index in 1 -day-old chickens >1.6) was 100%.

View Article: PubMed Central - PubMed

Affiliation: Key Laboratory of Microbiology, School of Life Science, Heilongjiang University, Harbin, People's Republic of China.

ABSTRACT
Layered double hydroxide (LDH)@SiO2 nanoparticles were developed as a delivery carrier for the plasmid DNA expressing the Newcastle disease virus F gene. The LDH was hydrotalcite-like materials. The plasmid DNA encapsulated in the LDH@SiO2 nanoparticles (pFDNA-LDH@SiO2-NPs) was prepared by the coprecipitation method, and the properties of pFDNA-LDH@SiO2-NPs were characterized by transmission electron microscopy, zeta potential analyzer, Fourier transform infrared spectroscopy, and X-ray diffraction analysis. The results demonstrated that the pFDNA-LDH@SiO2-NPs had a regular morphology and high stability with a mean diameter of 371.93 nm, loading capacity of 39.66%±0.45%, and a zeta potential of +31.63 mV. A release assay in vitro showed that up to 91.36% of the total plasmid DNA could be sustainably released from the pFDNA-LDH@SiO2-NPs within 288 hours. The LDH@SiO2 nanoparticles had very low toxicity. Additionally, their high transfection efficiency in vitro was detected by fluorescent microscopy. Intranasal immunization of specific pathogen-free chickens with pFDNA-LDH@SiO2-NPs induced stronger cellular, humoral, and mucosal immune responses and achieved a greater sustained release effect than intramuscular naked plasmid DNA, and the protective efficacy after challenge with the strain F48E9 with highly virulent (mean death time of chicken embryos ≤60 hours, intracerebral pathogenicity index in 1 -day-old chickens >1.6) was 100%. Based on the results, LDH@SiO2 nanoparticles can be used as a delivery carrier for mucosal immunity of Newcastle disease DNA vaccine, and have great application potential in the future.

No MeSH data available.


Related in: MedlinePlus