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Synthesis, characterization, and immune efficacy of layered double hydroxide@SiO2 nanoparticles with shell-core structure as a delivery carrier for Newcastle disease virus DNA vaccine.

Zhao K, Rong G, Guo C, Luo X, Kang H, Sun Y, Dai C, Wang X, Wang X, Jin Z, Cui S, Sun Q - Int J Nanomedicine (2015)

Bottom Line: The results demonstrated that the pFDNA-LDH@SiO2-NPs had a regular morphology and high stability with a mean diameter of 371.93 nm, loading capacity of 39.66%±0.45%, and a zeta potential of +31.63 mV.Additionally, their high transfection efficiency in vitro was detected by fluorescent microscopy.Intranasal immunization of specific pathogen-free chickens with pFDNA-LDH@SiO2-NPs induced stronger cellular, humoral, and mucosal immune responses and achieved a greater sustained release effect than intramuscular naked plasmid DNA, and the protective efficacy after challenge with the strain F48E9 with highly virulent (mean death time of chicken embryos ≤60 hours, intracerebral pathogenicity index in 1 -day-old chickens >1.6) was 100%.

View Article: PubMed Central - PubMed

Affiliation: Key Laboratory of Microbiology, School of Life Science, Heilongjiang University, Harbin, People's Republic of China.

ABSTRACT
Layered double hydroxide (LDH)@SiO2 nanoparticles were developed as a delivery carrier for the plasmid DNA expressing the Newcastle disease virus F gene. The LDH was hydrotalcite-like materials. The plasmid DNA encapsulated in the LDH@SiO2 nanoparticles (pFDNA-LDH@SiO2-NPs) was prepared by the coprecipitation method, and the properties of pFDNA-LDH@SiO2-NPs were characterized by transmission electron microscopy, zeta potential analyzer, Fourier transform infrared spectroscopy, and X-ray diffraction analysis. The results demonstrated that the pFDNA-LDH@SiO2-NPs had a regular morphology and high stability with a mean diameter of 371.93 nm, loading capacity of 39.66%±0.45%, and a zeta potential of +31.63 mV. A release assay in vitro showed that up to 91.36% of the total plasmid DNA could be sustainably released from the pFDNA-LDH@SiO2-NPs within 288 hours. The LDH@SiO2 nanoparticles had very low toxicity. Additionally, their high transfection efficiency in vitro was detected by fluorescent microscopy. Intranasal immunization of specific pathogen-free chickens with pFDNA-LDH@SiO2-NPs induced stronger cellular, humoral, and mucosal immune responses and achieved a greater sustained release effect than intramuscular naked plasmid DNA, and the protective efficacy after challenge with the strain F48E9 with highly virulent (mean death time of chicken embryos ≤60 hours, intracerebral pathogenicity index in 1 -day-old chickens >1.6) was 100%. Based on the results, LDH@SiO2 nanoparticles can be used as a delivery carrier for mucosal immunity of Newcastle disease DNA vaccine, and have great application potential in the future.

No MeSH data available.


Related in: MedlinePlus

In vitro release profiles of DNA from pFDNA-LDH@SiO2-NPs in phosphate-buffered saline (pH 7.4).Notes: The experiment was repeated three times and measured in triplicate. Data are presented as the mean ± standard deviation (n=3).Abbreviations: LDH, layered double hydroxide; NPs, nanoparticles; pFDNA-LDH@SiO2-NPs, Newcastle disease virus F gene encapsulated in LDH@SiO2-NPs.
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f6-ijn-10-2895: In vitro release profiles of DNA from pFDNA-LDH@SiO2-NPs in phosphate-buffered saline (pH 7.4).Notes: The experiment was repeated three times and measured in triplicate. Data are presented as the mean ± standard deviation (n=3).Abbreviations: LDH, layered double hydroxide; NPs, nanoparticles; pFDNA-LDH@SiO2-NPs, Newcastle disease virus F gene encapsulated in LDH@SiO2-NPs.

Mentions: As shown in Figure 6, the amount of DNA released from the pFDNA-LDH@SiO2-NPs was increased to 47.36%±1.95% (n=3) from 0 to 48 hours, revealing that the burst release mainly takes place during the first 48 hours and is followed by a slow and continuous release, maintained for longer than 96 hours, reaching 64.27%±1.52% but not changing significantly after 96 hours. At 288 hours, the amount of plasmid DNA released from the pFDNA-SiO2-NPs reached 91.36%±1.1%.


Synthesis, characterization, and immune efficacy of layered double hydroxide@SiO2 nanoparticles with shell-core structure as a delivery carrier for Newcastle disease virus DNA vaccine.

Zhao K, Rong G, Guo C, Luo X, Kang H, Sun Y, Dai C, Wang X, Wang X, Jin Z, Cui S, Sun Q - Int J Nanomedicine (2015)

In vitro release profiles of DNA from pFDNA-LDH@SiO2-NPs in phosphate-buffered saline (pH 7.4).Notes: The experiment was repeated three times and measured in triplicate. Data are presented as the mean ± standard deviation (n=3).Abbreviations: LDH, layered double hydroxide; NPs, nanoparticles; pFDNA-LDH@SiO2-NPs, Newcastle disease virus F gene encapsulated in LDH@SiO2-NPs.
© Copyright Policy
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4403701&req=5

f6-ijn-10-2895: In vitro release profiles of DNA from pFDNA-LDH@SiO2-NPs in phosphate-buffered saline (pH 7.4).Notes: The experiment was repeated three times and measured in triplicate. Data are presented as the mean ± standard deviation (n=3).Abbreviations: LDH, layered double hydroxide; NPs, nanoparticles; pFDNA-LDH@SiO2-NPs, Newcastle disease virus F gene encapsulated in LDH@SiO2-NPs.
Mentions: As shown in Figure 6, the amount of DNA released from the pFDNA-LDH@SiO2-NPs was increased to 47.36%±1.95% (n=3) from 0 to 48 hours, revealing that the burst release mainly takes place during the first 48 hours and is followed by a slow and continuous release, maintained for longer than 96 hours, reaching 64.27%±1.52% but not changing significantly after 96 hours. At 288 hours, the amount of plasmid DNA released from the pFDNA-SiO2-NPs reached 91.36%±1.1%.

Bottom Line: The results demonstrated that the pFDNA-LDH@SiO2-NPs had a regular morphology and high stability with a mean diameter of 371.93 nm, loading capacity of 39.66%±0.45%, and a zeta potential of +31.63 mV.Additionally, their high transfection efficiency in vitro was detected by fluorescent microscopy.Intranasal immunization of specific pathogen-free chickens with pFDNA-LDH@SiO2-NPs induced stronger cellular, humoral, and mucosal immune responses and achieved a greater sustained release effect than intramuscular naked plasmid DNA, and the protective efficacy after challenge with the strain F48E9 with highly virulent (mean death time of chicken embryos ≤60 hours, intracerebral pathogenicity index in 1 -day-old chickens >1.6) was 100%.

View Article: PubMed Central - PubMed

Affiliation: Key Laboratory of Microbiology, School of Life Science, Heilongjiang University, Harbin, People's Republic of China.

ABSTRACT
Layered double hydroxide (LDH)@SiO2 nanoparticles were developed as a delivery carrier for the plasmid DNA expressing the Newcastle disease virus F gene. The LDH was hydrotalcite-like materials. The plasmid DNA encapsulated in the LDH@SiO2 nanoparticles (pFDNA-LDH@SiO2-NPs) was prepared by the coprecipitation method, and the properties of pFDNA-LDH@SiO2-NPs were characterized by transmission electron microscopy, zeta potential analyzer, Fourier transform infrared spectroscopy, and X-ray diffraction analysis. The results demonstrated that the pFDNA-LDH@SiO2-NPs had a regular morphology and high stability with a mean diameter of 371.93 nm, loading capacity of 39.66%±0.45%, and a zeta potential of +31.63 mV. A release assay in vitro showed that up to 91.36% of the total plasmid DNA could be sustainably released from the pFDNA-LDH@SiO2-NPs within 288 hours. The LDH@SiO2 nanoparticles had very low toxicity. Additionally, their high transfection efficiency in vitro was detected by fluorescent microscopy. Intranasal immunization of specific pathogen-free chickens with pFDNA-LDH@SiO2-NPs induced stronger cellular, humoral, and mucosal immune responses and achieved a greater sustained release effect than intramuscular naked plasmid DNA, and the protective efficacy after challenge with the strain F48E9 with highly virulent (mean death time of chicken embryos ≤60 hours, intracerebral pathogenicity index in 1 -day-old chickens >1.6) was 100%. Based on the results, LDH@SiO2 nanoparticles can be used as a delivery carrier for mucosal immunity of Newcastle disease DNA vaccine, and have great application potential in the future.

No MeSH data available.


Related in: MedlinePlus