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Synthesis, characterization, and immune efficacy of layered double hydroxide@SiO2 nanoparticles with shell-core structure as a delivery carrier for Newcastle disease virus DNA vaccine.

Zhao K, Rong G, Guo C, Luo X, Kang H, Sun Y, Dai C, Wang X, Wang X, Jin Z, Cui S, Sun Q - Int J Nanomedicine (2015)

Bottom Line: The results demonstrated that the pFDNA-LDH@SiO2-NPs had a regular morphology and high stability with a mean diameter of 371.93 nm, loading capacity of 39.66%±0.45%, and a zeta potential of +31.63 mV.Additionally, their high transfection efficiency in vitro was detected by fluorescent microscopy.Intranasal immunization of specific pathogen-free chickens with pFDNA-LDH@SiO2-NPs induced stronger cellular, humoral, and mucosal immune responses and achieved a greater sustained release effect than intramuscular naked plasmid DNA, and the protective efficacy after challenge with the strain F48E9 with highly virulent (mean death time of chicken embryos ≤60 hours, intracerebral pathogenicity index in 1 -day-old chickens >1.6) was 100%.

View Article: PubMed Central - PubMed

Affiliation: Key Laboratory of Microbiology, School of Life Science, Heilongjiang University, Harbin, People's Republic of China.

ABSTRACT
Layered double hydroxide (LDH)@SiO2 nanoparticles were developed as a delivery carrier for the plasmid DNA expressing the Newcastle disease virus F gene. The LDH was hydrotalcite-like materials. The plasmid DNA encapsulated in the LDH@SiO2 nanoparticles (pFDNA-LDH@SiO2-NPs) was prepared by the coprecipitation method, and the properties of pFDNA-LDH@SiO2-NPs were characterized by transmission electron microscopy, zeta potential analyzer, Fourier transform infrared spectroscopy, and X-ray diffraction analysis. The results demonstrated that the pFDNA-LDH@SiO2-NPs had a regular morphology and high stability with a mean diameter of 371.93 nm, loading capacity of 39.66%±0.45%, and a zeta potential of +31.63 mV. A release assay in vitro showed that up to 91.36% of the total plasmid DNA could be sustainably released from the pFDNA-LDH@SiO2-NPs within 288 hours. The LDH@SiO2 nanoparticles had very low toxicity. Additionally, their high transfection efficiency in vitro was detected by fluorescent microscopy. Intranasal immunization of specific pathogen-free chickens with pFDNA-LDH@SiO2-NPs induced stronger cellular, humoral, and mucosal immune responses and achieved a greater sustained release effect than intramuscular naked plasmid DNA, and the protective efficacy after challenge with the strain F48E9 with highly virulent (mean death time of chicken embryos ≤60 hours, intracerebral pathogenicity index in 1 -day-old chickens >1.6) was 100%. Based on the results, LDH@SiO2 nanoparticles can be used as a delivery carrier for mucosal immunity of Newcastle disease DNA vaccine, and have great application potential in the future.

No MeSH data available.


Related in: MedlinePlus

Detection of SiO2 nanoparticles loaded with plasmid DNA for connection, release, and protection properties.Notes: (A) Agarose gel electrophoresis of the amino-modified silica-DNA complexes. Maker (M): DL 15,000 marker. Lane 1: plasmid DNA. Lanes 2–8: the mass ratios of SiO2 to plasmid DNA were 1:5, 1:1, 5:1, 10:1, 20:1, 30:1, and 40:1, respectively. (B) Stability analysis of the plasmid DNA binding with SiO2 nanoparticles. (C) Analysis of protection of the plasmid DNA from DNase I digestion. Maker (M): DL 15,000 marker. Lane 1: plasmid DNA. Lanes 2–8: the mass ratios of SiO2 to plasmid DNA were 1:5, 1:1, 5:1, 10:1, 20:1, 30:1, and 40:1, respectively.
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f2-ijn-10-2895: Detection of SiO2 nanoparticles loaded with plasmid DNA for connection, release, and protection properties.Notes: (A) Agarose gel electrophoresis of the amino-modified silica-DNA complexes. Maker (M): DL 15,000 marker. Lane 1: plasmid DNA. Lanes 2–8: the mass ratios of SiO2 to plasmid DNA were 1:5, 1:1, 5:1, 10:1, 20:1, 30:1, and 40:1, respectively. (B) Stability analysis of the plasmid DNA binding with SiO2 nanoparticles. (C) Analysis of protection of the plasmid DNA from DNase I digestion. Maker (M): DL 15,000 marker. Lane 1: plasmid DNA. Lanes 2–8: the mass ratios of SiO2 to plasmid DNA were 1:5, 1:1, 5:1, 10:1, 20:1, 30:1, and 40:1, respectively.

Mentions: Figure 2A shows the agarose gel electrophoresis image for the pFDNA-SiO2-NP complexes, which demonstrated the ability of SiO2 NPs to immobilize DNA with high efficiency. Through changing the number of particles, we could adjust the amounts of DNA binding to the particle surface.


Synthesis, characterization, and immune efficacy of layered double hydroxide@SiO2 nanoparticles with shell-core structure as a delivery carrier for Newcastle disease virus DNA vaccine.

Zhao K, Rong G, Guo C, Luo X, Kang H, Sun Y, Dai C, Wang X, Wang X, Jin Z, Cui S, Sun Q - Int J Nanomedicine (2015)

Detection of SiO2 nanoparticles loaded with plasmid DNA for connection, release, and protection properties.Notes: (A) Agarose gel electrophoresis of the amino-modified silica-DNA complexes. Maker (M): DL 15,000 marker. Lane 1: plasmid DNA. Lanes 2–8: the mass ratios of SiO2 to plasmid DNA were 1:5, 1:1, 5:1, 10:1, 20:1, 30:1, and 40:1, respectively. (B) Stability analysis of the plasmid DNA binding with SiO2 nanoparticles. (C) Analysis of protection of the plasmid DNA from DNase I digestion. Maker (M): DL 15,000 marker. Lane 1: plasmid DNA. Lanes 2–8: the mass ratios of SiO2 to plasmid DNA were 1:5, 1:1, 5:1, 10:1, 20:1, 30:1, and 40:1, respectively.
© Copyright Policy
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4403701&req=5

f2-ijn-10-2895: Detection of SiO2 nanoparticles loaded with plasmid DNA for connection, release, and protection properties.Notes: (A) Agarose gel electrophoresis of the amino-modified silica-DNA complexes. Maker (M): DL 15,000 marker. Lane 1: plasmid DNA. Lanes 2–8: the mass ratios of SiO2 to plasmid DNA were 1:5, 1:1, 5:1, 10:1, 20:1, 30:1, and 40:1, respectively. (B) Stability analysis of the plasmid DNA binding with SiO2 nanoparticles. (C) Analysis of protection of the plasmid DNA from DNase I digestion. Maker (M): DL 15,000 marker. Lane 1: plasmid DNA. Lanes 2–8: the mass ratios of SiO2 to plasmid DNA were 1:5, 1:1, 5:1, 10:1, 20:1, 30:1, and 40:1, respectively.
Mentions: Figure 2A shows the agarose gel electrophoresis image for the pFDNA-SiO2-NP complexes, which demonstrated the ability of SiO2 NPs to immobilize DNA with high efficiency. Through changing the number of particles, we could adjust the amounts of DNA binding to the particle surface.

Bottom Line: The results demonstrated that the pFDNA-LDH@SiO2-NPs had a regular morphology and high stability with a mean diameter of 371.93 nm, loading capacity of 39.66%±0.45%, and a zeta potential of +31.63 mV.Additionally, their high transfection efficiency in vitro was detected by fluorescent microscopy.Intranasal immunization of specific pathogen-free chickens with pFDNA-LDH@SiO2-NPs induced stronger cellular, humoral, and mucosal immune responses and achieved a greater sustained release effect than intramuscular naked plasmid DNA, and the protective efficacy after challenge with the strain F48E9 with highly virulent (mean death time of chicken embryos ≤60 hours, intracerebral pathogenicity index in 1 -day-old chickens >1.6) was 100%.

View Article: PubMed Central - PubMed

Affiliation: Key Laboratory of Microbiology, School of Life Science, Heilongjiang University, Harbin, People's Republic of China.

ABSTRACT
Layered double hydroxide (LDH)@SiO2 nanoparticles were developed as a delivery carrier for the plasmid DNA expressing the Newcastle disease virus F gene. The LDH was hydrotalcite-like materials. The plasmid DNA encapsulated in the LDH@SiO2 nanoparticles (pFDNA-LDH@SiO2-NPs) was prepared by the coprecipitation method, and the properties of pFDNA-LDH@SiO2-NPs were characterized by transmission electron microscopy, zeta potential analyzer, Fourier transform infrared spectroscopy, and X-ray diffraction analysis. The results demonstrated that the pFDNA-LDH@SiO2-NPs had a regular morphology and high stability with a mean diameter of 371.93 nm, loading capacity of 39.66%±0.45%, and a zeta potential of +31.63 mV. A release assay in vitro showed that up to 91.36% of the total plasmid DNA could be sustainably released from the pFDNA-LDH@SiO2-NPs within 288 hours. The LDH@SiO2 nanoparticles had very low toxicity. Additionally, their high transfection efficiency in vitro was detected by fluorescent microscopy. Intranasal immunization of specific pathogen-free chickens with pFDNA-LDH@SiO2-NPs induced stronger cellular, humoral, and mucosal immune responses and achieved a greater sustained release effect than intramuscular naked plasmid DNA, and the protective efficacy after challenge with the strain F48E9 with highly virulent (mean death time of chicken embryos ≤60 hours, intracerebral pathogenicity index in 1 -day-old chickens >1.6) was 100%. Based on the results, LDH@SiO2 nanoparticles can be used as a delivery carrier for mucosal immunity of Newcastle disease DNA vaccine, and have great application potential in the future.

No MeSH data available.


Related in: MedlinePlus