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Synthesis, characterization, and immune efficacy of layered double hydroxide@SiO2 nanoparticles with shell-core structure as a delivery carrier for Newcastle disease virus DNA vaccine.

Zhao K, Rong G, Guo C, Luo X, Kang H, Sun Y, Dai C, Wang X, Wang X, Jin Z, Cui S, Sun Q - Int J Nanomedicine (2015)

Bottom Line: The results demonstrated that the pFDNA-LDH@SiO2-NPs had a regular morphology and high stability with a mean diameter of 371.93 nm, loading capacity of 39.66%±0.45%, and a zeta potential of +31.63 mV.Additionally, their high transfection efficiency in vitro was detected by fluorescent microscopy.Intranasal immunization of specific pathogen-free chickens with pFDNA-LDH@SiO2-NPs induced stronger cellular, humoral, and mucosal immune responses and achieved a greater sustained release effect than intramuscular naked plasmid DNA, and the protective efficacy after challenge with the strain F48E9 with highly virulent (mean death time of chicken embryos ≤60 hours, intracerebral pathogenicity index in 1 -day-old chickens >1.6) was 100%.

View Article: PubMed Central - PubMed

Affiliation: Key Laboratory of Microbiology, School of Life Science, Heilongjiang University, Harbin, People's Republic of China.

ABSTRACT
Layered double hydroxide (LDH)@SiO2 nanoparticles were developed as a delivery carrier for the plasmid DNA expressing the Newcastle disease virus F gene. The LDH was hydrotalcite-like materials. The plasmid DNA encapsulated in the LDH@SiO2 nanoparticles (pFDNA-LDH@SiO2-NPs) was prepared by the coprecipitation method, and the properties of pFDNA-LDH@SiO2-NPs were characterized by transmission electron microscopy, zeta potential analyzer, Fourier transform infrared spectroscopy, and X-ray diffraction analysis. The results demonstrated that the pFDNA-LDH@SiO2-NPs had a regular morphology and high stability with a mean diameter of 371.93 nm, loading capacity of 39.66%±0.45%, and a zeta potential of +31.63 mV. A release assay in vitro showed that up to 91.36% of the total plasmid DNA could be sustainably released from the pFDNA-LDH@SiO2-NPs within 288 hours. The LDH@SiO2 nanoparticles had very low toxicity. Additionally, their high transfection efficiency in vitro was detected by fluorescent microscopy. Intranasal immunization of specific pathogen-free chickens with pFDNA-LDH@SiO2-NPs induced stronger cellular, humoral, and mucosal immune responses and achieved a greater sustained release effect than intramuscular naked plasmid DNA, and the protective efficacy after challenge with the strain F48E9 with highly virulent (mean death time of chicken embryos ≤60 hours, intracerebral pathogenicity index in 1 -day-old chickens >1.6) was 100%. Based on the results, LDH@SiO2 nanoparticles can be used as a delivery carrier for mucosal immunity of Newcastle disease DNA vaccine, and have great application potential in the future.

No MeSH data available.


Related in: MedlinePlus

Histopathological analyses of the duodena and myocardia of chickens challenged with the highly virulent NDV strain F48E9.Notes: (A) Tissues of the duodena from chickens immunized with pFDNA-LDH@SiO2-NPs IN. (B) Tissues of the myocardia from chickens immunized with pFDNA-LDH@SiO2-NPs IN. (C) Tissues of the duodena from chickens immunized with pFDNA-LDH@SiO2-NPs IM, naked plasmid pVAX1-F(o) DNA IM, or PBS IM. (D) Tissues of the myocardia from chickens in groups immunized with the pFDNA-LDH@SiO2-NPs IM, naked plasmid pVAX1-F(o) DNA IM, or PBS IM.Abbreviations: IM, intramuscularly; IN, intranasally; LDH, layered double hydroxide; NDV, Newcastle disease virus; NPs, nanoparticles; PBS, phosphate-buffered saline; pFDNA-LDH@SiO2-NPs, NDV F gene encapsulated in the LDH@SiO2-NPs.
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f10-ijn-10-2895: Histopathological analyses of the duodena and myocardia of chickens challenged with the highly virulent NDV strain F48E9.Notes: (A) Tissues of the duodena from chickens immunized with pFDNA-LDH@SiO2-NPs IN. (B) Tissues of the myocardia from chickens immunized with pFDNA-LDH@SiO2-NPs IN. (C) Tissues of the duodena from chickens immunized with pFDNA-LDH@SiO2-NPs IM, naked plasmid pVAX1-F(o) DNA IM, or PBS IM. (D) Tissues of the myocardia from chickens in groups immunized with the pFDNA-LDH@SiO2-NPs IM, naked plasmid pVAX1-F(o) DNA IM, or PBS IM.Abbreviations: IM, intramuscularly; IN, intranasally; LDH, layered double hydroxide; NDV, Newcastle disease virus; NPs, nanoparticles; PBS, phosphate-buffered saline; pFDNA-LDH@SiO2-NPs, NDV F gene encapsulated in the LDH@SiO2-NPs.

Mentions: Within 2–5 days, the highly virulent NDV strain F48E9 had killed all the chickens treated with either PBS IM or blank LDH@SiO2-NPs IM (Table 3). In contrast, strain F48E9 killed only 60%, 40%, and 0% of the chickens treated with the naked plasmid pVAX1-F(o) DNA IM, pFDNA-LDH@SiO2-NPs IM, and pFDNA-LDH@SiO2-NPs IN, respectively (Table 3). Two chickens of the pFDNA-LDH@SiO2-NPs IM group died, and the protective efficacy was 60%. There were no clinical signs in chickens immunized with the pFDNA-LDH@SiO2-NPs IN after being challenged with the strain F48E9, and no pathological and histopathological changes were found in their duodena (Figure 10A) and myocardia (Figure 10B).


Synthesis, characterization, and immune efficacy of layered double hydroxide@SiO2 nanoparticles with shell-core structure as a delivery carrier for Newcastle disease virus DNA vaccine.

Zhao K, Rong G, Guo C, Luo X, Kang H, Sun Y, Dai C, Wang X, Wang X, Jin Z, Cui S, Sun Q - Int J Nanomedicine (2015)

Histopathological analyses of the duodena and myocardia of chickens challenged with the highly virulent NDV strain F48E9.Notes: (A) Tissues of the duodena from chickens immunized with pFDNA-LDH@SiO2-NPs IN. (B) Tissues of the myocardia from chickens immunized with pFDNA-LDH@SiO2-NPs IN. (C) Tissues of the duodena from chickens immunized with pFDNA-LDH@SiO2-NPs IM, naked plasmid pVAX1-F(o) DNA IM, or PBS IM. (D) Tissues of the myocardia from chickens in groups immunized with the pFDNA-LDH@SiO2-NPs IM, naked plasmid pVAX1-F(o) DNA IM, or PBS IM.Abbreviations: IM, intramuscularly; IN, intranasally; LDH, layered double hydroxide; NDV, Newcastle disease virus; NPs, nanoparticles; PBS, phosphate-buffered saline; pFDNA-LDH@SiO2-NPs, NDV F gene encapsulated in the LDH@SiO2-NPs.
© Copyright Policy
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4403701&req=5

f10-ijn-10-2895: Histopathological analyses of the duodena and myocardia of chickens challenged with the highly virulent NDV strain F48E9.Notes: (A) Tissues of the duodena from chickens immunized with pFDNA-LDH@SiO2-NPs IN. (B) Tissues of the myocardia from chickens immunized with pFDNA-LDH@SiO2-NPs IN. (C) Tissues of the duodena from chickens immunized with pFDNA-LDH@SiO2-NPs IM, naked plasmid pVAX1-F(o) DNA IM, or PBS IM. (D) Tissues of the myocardia from chickens in groups immunized with the pFDNA-LDH@SiO2-NPs IM, naked plasmid pVAX1-F(o) DNA IM, or PBS IM.Abbreviations: IM, intramuscularly; IN, intranasally; LDH, layered double hydroxide; NDV, Newcastle disease virus; NPs, nanoparticles; PBS, phosphate-buffered saline; pFDNA-LDH@SiO2-NPs, NDV F gene encapsulated in the LDH@SiO2-NPs.
Mentions: Within 2–5 days, the highly virulent NDV strain F48E9 had killed all the chickens treated with either PBS IM or blank LDH@SiO2-NPs IM (Table 3). In contrast, strain F48E9 killed only 60%, 40%, and 0% of the chickens treated with the naked plasmid pVAX1-F(o) DNA IM, pFDNA-LDH@SiO2-NPs IM, and pFDNA-LDH@SiO2-NPs IN, respectively (Table 3). Two chickens of the pFDNA-LDH@SiO2-NPs IM group died, and the protective efficacy was 60%. There were no clinical signs in chickens immunized with the pFDNA-LDH@SiO2-NPs IN after being challenged with the strain F48E9, and no pathological and histopathological changes were found in their duodena (Figure 10A) and myocardia (Figure 10B).

Bottom Line: The results demonstrated that the pFDNA-LDH@SiO2-NPs had a regular morphology and high stability with a mean diameter of 371.93 nm, loading capacity of 39.66%±0.45%, and a zeta potential of +31.63 mV.Additionally, their high transfection efficiency in vitro was detected by fluorescent microscopy.Intranasal immunization of specific pathogen-free chickens with pFDNA-LDH@SiO2-NPs induced stronger cellular, humoral, and mucosal immune responses and achieved a greater sustained release effect than intramuscular naked plasmid DNA, and the protective efficacy after challenge with the strain F48E9 with highly virulent (mean death time of chicken embryos ≤60 hours, intracerebral pathogenicity index in 1 -day-old chickens >1.6) was 100%.

View Article: PubMed Central - PubMed

Affiliation: Key Laboratory of Microbiology, School of Life Science, Heilongjiang University, Harbin, People's Republic of China.

ABSTRACT
Layered double hydroxide (LDH)@SiO2 nanoparticles were developed as a delivery carrier for the plasmid DNA expressing the Newcastle disease virus F gene. The LDH was hydrotalcite-like materials. The plasmid DNA encapsulated in the LDH@SiO2 nanoparticles (pFDNA-LDH@SiO2-NPs) was prepared by the coprecipitation method, and the properties of pFDNA-LDH@SiO2-NPs were characterized by transmission electron microscopy, zeta potential analyzer, Fourier transform infrared spectroscopy, and X-ray diffraction analysis. The results demonstrated that the pFDNA-LDH@SiO2-NPs had a regular morphology and high stability with a mean diameter of 371.93 nm, loading capacity of 39.66%±0.45%, and a zeta potential of +31.63 mV. A release assay in vitro showed that up to 91.36% of the total plasmid DNA could be sustainably released from the pFDNA-LDH@SiO2-NPs within 288 hours. The LDH@SiO2 nanoparticles had very low toxicity. Additionally, their high transfection efficiency in vitro was detected by fluorescent microscopy. Intranasal immunization of specific pathogen-free chickens with pFDNA-LDH@SiO2-NPs induced stronger cellular, humoral, and mucosal immune responses and achieved a greater sustained release effect than intramuscular naked plasmid DNA, and the protective efficacy after challenge with the strain F48E9 with highly virulent (mean death time of chicken embryos ≤60 hours, intracerebral pathogenicity index in 1 -day-old chickens >1.6) was 100%. Based on the results, LDH@SiO2 nanoparticles can be used as a delivery carrier for mucosal immunity of Newcastle disease DNA vaccine, and have great application potential in the future.

No MeSH data available.


Related in: MedlinePlus