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Coadministration of doxorubicin and etoposide loaded in camel milk phospholipids liposomes showed increased antitumor activity in a murine model.

Maswadeh HM, Aljarbou AN, Alorainy MS, Rahmani AH, Khan MA - Int J Nanomedicine (2015)

Bottom Line: Tumor-bearing mice treated with a combination of Dox and ETP loaded into CML-Lip showed increased survival and reduced tumor growth compared to other groups, including the combination of Dox and ETP in DPPC-Lip.Fibrosarcoma-bearing mice treated with a combination of free (Dox + ETP) showed much higher tumor growth compared to those groups treated with CML-Lip-(Dox + ETP) or DPPC-Lip-(Dox + ETP).Immunohistochemical study was also performed to show the expression of tumor-suppressor PTEN, and it was found that the tumor tissues from the group of mice treated with a combination of free (Dox + ETP) showed greater loss of cytoplasmic PTEN than tumor tissues obtained from the groups of mice treated with CML-Lip-(Dox + ETP) or DPPC-Lip-(Dox + ETP).

View Article: PubMed Central - PubMed

Affiliation: Department of Pharmaceutics, College of Pharmacy, Qassim University, Buraydah, Kingdom of Saudi Arabia.

ABSTRACT
Small unilamellar vesicles from camel milk phospholipids (CML) mixture or from 1,2 dipalmitoyl-sn-glycero-3-phosphatidylcholine (DPPC) were prepared, and anticancer drugs doxorubicin (Dox) or etoposide (ETP) were loaded. Liposomal formulations were used against fibrosarcoma in a murine model. Results showed a very high percentage of Dox encapsulation (~98%) in liposomes (Lip) prepared from CML-Lip or DPPC-Lip, whereas the percentage of encapsulations of ETP was on the lower side, 22% of CML-Lip and 18% for DPPC-Lip. Differential scanning calorimetry curves show that Dox enhances the lamellar formation in CML-Lip, whereas ETP enhances the nonlamellar formation. Differential scanning calorimetry curves also showed that the presence of Dox and ETP together into DPPC-Lip produced the interdigitation effect. The in vivo anticancer activity of liposomal formulations of Dox or ETP or a combination of both was assessed against benzopyrene (BAP)-induced fibrosarcoma in a murine model. Tumor-bearing mice treated with a combination of Dox and ETP loaded into CML-Lip showed increased survival and reduced tumor growth compared to other groups, including the combination of Dox and ETP in DPPC-Lip. Fibrosarcoma-bearing mice treated with a combination of free (Dox + ETP) showed much higher tumor growth compared to those groups treated with CML-Lip-(Dox + ETP) or DPPC-Lip-(Dox + ETP). Immunohistochemical study was also performed to show the expression of tumor-suppressor PTEN, and it was found that the tumor tissues from the group of mice treated with a combination of free (Dox + ETP) showed greater loss of cytoplasmic PTEN than tumor tissues obtained from the groups of mice treated with CML-Lip-(Dox + ETP) or DPPC-Lip-(Dox + ETP).

No MeSH data available.


Related in: MedlinePlus

Effects of liposomal formulations of etoposide and doxorubicin against BAP-induced tumors.Notes: Mice treated with (A) DPPC-Lip-(Dox + ETP), (B) CML-Lip-(Dox + ETP), or showed delayed tumor growth as compared to free drugs (P<0.001); Data are values ± SD (n=10 at initiation of therapy; the number varies at later time points due some mortalities).Abbreviations: BAP, benzopyrene; CML-Lip, camel milk phospholipids liposomes; Dox, doxorubicin; DPPC-Lip, 1,2 dipalmitoyl-sn-glycero-3-phosphatidylcholine liposomes; ETP, etoposide; SD, standard deviation.
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f4-ijn-10-2847: Effects of liposomal formulations of etoposide and doxorubicin against BAP-induced tumors.Notes: Mice treated with (A) DPPC-Lip-(Dox + ETP), (B) CML-Lip-(Dox + ETP), or showed delayed tumor growth as compared to free drugs (P<0.001); Data are values ± SD (n=10 at initiation of therapy; the number varies at later time points due some mortalities).Abbreviations: BAP, benzopyrene; CML-Lip, camel milk phospholipids liposomes; Dox, doxorubicin; DPPC-Lip, 1,2 dipalmitoyl-sn-glycero-3-phosphatidylcholine liposomes; ETP, etoposide; SD, standard deviation.

Mentions: The in vivo activity of formulations of Dox or ETP or a combination of both was assessed against fibrosarcoma in a murine model. Chemotherapy was started when tumors attained the size in the range of 150–200 mm3. Fibrosarcoma-bearing mice were treated with a single weekly dose of 5 mg/kg of free or liposomal formulations of Dox or ETP or a combination of both for 3 weeks. Among the all treatment groups, the tumors showed the least progression or delayed growth in the group of mice treated with CML-Lip-(Dox + ETP) compared to the groups treated with free (Dox + ETP) or DPPC-Lip-(Dox + ETP) (Figure 4A and B). Once chemotherapy was stopped, there was a sharp progression in the size of tumors, particularly in the groups of mice treated with free formulations of drugs (Figure 4A and B).


Coadministration of doxorubicin and etoposide loaded in camel milk phospholipids liposomes showed increased antitumor activity in a murine model.

Maswadeh HM, Aljarbou AN, Alorainy MS, Rahmani AH, Khan MA - Int J Nanomedicine (2015)

Effects of liposomal formulations of etoposide and doxorubicin against BAP-induced tumors.Notes: Mice treated with (A) DPPC-Lip-(Dox + ETP), (B) CML-Lip-(Dox + ETP), or showed delayed tumor growth as compared to free drugs (P<0.001); Data are values ± SD (n=10 at initiation of therapy; the number varies at later time points due some mortalities).Abbreviations: BAP, benzopyrene; CML-Lip, camel milk phospholipids liposomes; Dox, doxorubicin; DPPC-Lip, 1,2 dipalmitoyl-sn-glycero-3-phosphatidylcholine liposomes; ETP, etoposide; SD, standard deviation.
© Copyright Policy
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4403685&req=5

f4-ijn-10-2847: Effects of liposomal formulations of etoposide and doxorubicin against BAP-induced tumors.Notes: Mice treated with (A) DPPC-Lip-(Dox + ETP), (B) CML-Lip-(Dox + ETP), or showed delayed tumor growth as compared to free drugs (P<0.001); Data are values ± SD (n=10 at initiation of therapy; the number varies at later time points due some mortalities).Abbreviations: BAP, benzopyrene; CML-Lip, camel milk phospholipids liposomes; Dox, doxorubicin; DPPC-Lip, 1,2 dipalmitoyl-sn-glycero-3-phosphatidylcholine liposomes; ETP, etoposide; SD, standard deviation.
Mentions: The in vivo activity of formulations of Dox or ETP or a combination of both was assessed against fibrosarcoma in a murine model. Chemotherapy was started when tumors attained the size in the range of 150–200 mm3. Fibrosarcoma-bearing mice were treated with a single weekly dose of 5 mg/kg of free or liposomal formulations of Dox or ETP or a combination of both for 3 weeks. Among the all treatment groups, the tumors showed the least progression or delayed growth in the group of mice treated with CML-Lip-(Dox + ETP) compared to the groups treated with free (Dox + ETP) or DPPC-Lip-(Dox + ETP) (Figure 4A and B). Once chemotherapy was stopped, there was a sharp progression in the size of tumors, particularly in the groups of mice treated with free formulations of drugs (Figure 4A and B).

Bottom Line: Tumor-bearing mice treated with a combination of Dox and ETP loaded into CML-Lip showed increased survival and reduced tumor growth compared to other groups, including the combination of Dox and ETP in DPPC-Lip.Fibrosarcoma-bearing mice treated with a combination of free (Dox + ETP) showed much higher tumor growth compared to those groups treated with CML-Lip-(Dox + ETP) or DPPC-Lip-(Dox + ETP).Immunohistochemical study was also performed to show the expression of tumor-suppressor PTEN, and it was found that the tumor tissues from the group of mice treated with a combination of free (Dox + ETP) showed greater loss of cytoplasmic PTEN than tumor tissues obtained from the groups of mice treated with CML-Lip-(Dox + ETP) or DPPC-Lip-(Dox + ETP).

View Article: PubMed Central - PubMed

Affiliation: Department of Pharmaceutics, College of Pharmacy, Qassim University, Buraydah, Kingdom of Saudi Arabia.

ABSTRACT
Small unilamellar vesicles from camel milk phospholipids (CML) mixture or from 1,2 dipalmitoyl-sn-glycero-3-phosphatidylcholine (DPPC) were prepared, and anticancer drugs doxorubicin (Dox) or etoposide (ETP) were loaded. Liposomal formulations were used against fibrosarcoma in a murine model. Results showed a very high percentage of Dox encapsulation (~98%) in liposomes (Lip) prepared from CML-Lip or DPPC-Lip, whereas the percentage of encapsulations of ETP was on the lower side, 22% of CML-Lip and 18% for DPPC-Lip. Differential scanning calorimetry curves show that Dox enhances the lamellar formation in CML-Lip, whereas ETP enhances the nonlamellar formation. Differential scanning calorimetry curves also showed that the presence of Dox and ETP together into DPPC-Lip produced the interdigitation effect. The in vivo anticancer activity of liposomal formulations of Dox or ETP or a combination of both was assessed against benzopyrene (BAP)-induced fibrosarcoma in a murine model. Tumor-bearing mice treated with a combination of Dox and ETP loaded into CML-Lip showed increased survival and reduced tumor growth compared to other groups, including the combination of Dox and ETP in DPPC-Lip. Fibrosarcoma-bearing mice treated with a combination of free (Dox + ETP) showed much higher tumor growth compared to those groups treated with CML-Lip-(Dox + ETP) or DPPC-Lip-(Dox + ETP). Immunohistochemical study was also performed to show the expression of tumor-suppressor PTEN, and it was found that the tumor tissues from the group of mice treated with a combination of free (Dox + ETP) showed greater loss of cytoplasmic PTEN than tumor tissues obtained from the groups of mice treated with CML-Lip-(Dox + ETP) or DPPC-Lip-(Dox + ETP).

No MeSH data available.


Related in: MedlinePlus