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Effect of Potassium Ions on Protoplast Generation during Yeast Induction from Mucor circinelloides Tieghem.

Omoifo CO - ISRN Biotechnol (2012)

Bottom Line: This paper confirms the induction of plurality of reproductive structures of the pleomorphic microorganism in minimal medium.On either side of this K(+) level, variation in morphologies, including neoplasts, protoplasts, septate hyphae, thallic, holothallic, and holoblastic conidia, was greater, although olive-green septate hyphae with vesicular conidiogenous apparatus occurred at all K(+) levels tested.This study suggested that following the establishment of transmembrane pH gradient across protoplast membrane, operation of Mitchellian proton pump was further promoted, thus leading to active transport mechanism, a prelude to yeast morphology induction.

View Article: PubMed Central - PubMed

Affiliation: Department of Crop Science, Ambrose Alli University, Ekpoma, Nigeria.

ABSTRACT
Mucor circinelloides aerobically exhibits coenocytic thallic growth habit with straight and circinate sporangiophores which culminate in globose or pyriform columellae enclosed within sporangial walls. It undergoes dimorphic switch with its conversion to multipolar budding yeast-like cells or thallic conidia. This paper confirms the induction of plurality of reproductive structures of the pleomorphic microorganism in minimal medium. Furthermore, construction of pH differentials at inflection points in the biphasic profiles during sporangiospore-yeast transformation indicated the intensity of H(+) release from intracellular medium of the growing microorganism in a study conducted with K(+) levels (0.0, 0.5, 0.7, 0.9, 1.0,1.10 g/L)-mediated broths. Optimum proton release was at 0.00 and 1.0 g/L K(+)-supplemented broths, but specific growth rate was least in the latter. It also coincided with a preponderance of neoplastic units, protoplasts, and terminal budding yeast cells. On either side of this K(+) level, variation in morphologies, including neoplasts, protoplasts, septate hyphae, thallic, holothallic, and holoblastic conidia, was greater, although olive-green septate hyphae with vesicular conidiogenous apparatus occurred at all K(+) levels tested. This study suggested that following the establishment of transmembrane pH gradient across protoplast membrane, operation of Mitchellian proton pump was further promoted, thus leading to active transport mechanism, a prelude to yeast morphology induction.

No MeSH data available.


Related in: MedlinePlus

Protoplasts and holoblastic conidia of M. circinelloides Tieghem coinduced in 1.10 g/L  K+-mediated, glucose-substrated multiionic broth at ×2000 mag.
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Related In: Results  -  Collection


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fig16: Protoplasts and holoblastic conidia of M. circinelloides Tieghem coinduced in 1.10 g/L  K+-mediated, glucose-substrated multiionic broth at ×2000 mag.

Mentions: Conspicuous globose to short-rod protoplasts were also induced in the 1.10 gg/L K+ medium (Figure 16). They were however not as numerous as it was in the preceding level of K+concentration. The holoblastic conidia (Figures 16 and 17) and holothallic conidia (Figure 17) assumed more robust form at this level of K+ medium. Also present were germlines, double-walled thallic (septate) growth, septate and olive-green filament with vesicular conidiogenous apparatus, and nascent/emergent yeast cells, which appeared as rods and ellipsoids. Figure 18 illustrated the various types of morphological forms of M. circinelloides as affected by K+ supplementation, observed in this study.


Effect of Potassium Ions on Protoplast Generation during Yeast Induction from Mucor circinelloides Tieghem.

Omoifo CO - ISRN Biotechnol (2012)

Protoplasts and holoblastic conidia of M. circinelloides Tieghem coinduced in 1.10 g/L  K+-mediated, glucose-substrated multiionic broth at ×2000 mag.
© Copyright Policy - open-access
Related In: Results  -  Collection

Show All Figures
getmorefigures.php?uid=PMC4403624&req=5

fig16: Protoplasts and holoblastic conidia of M. circinelloides Tieghem coinduced in 1.10 g/L  K+-mediated, glucose-substrated multiionic broth at ×2000 mag.
Mentions: Conspicuous globose to short-rod protoplasts were also induced in the 1.10 gg/L K+ medium (Figure 16). They were however not as numerous as it was in the preceding level of K+concentration. The holoblastic conidia (Figures 16 and 17) and holothallic conidia (Figure 17) assumed more robust form at this level of K+ medium. Also present were germlines, double-walled thallic (septate) growth, septate and olive-green filament with vesicular conidiogenous apparatus, and nascent/emergent yeast cells, which appeared as rods and ellipsoids. Figure 18 illustrated the various types of morphological forms of M. circinelloides as affected by K+ supplementation, observed in this study.

Bottom Line: This paper confirms the induction of plurality of reproductive structures of the pleomorphic microorganism in minimal medium.On either side of this K(+) level, variation in morphologies, including neoplasts, protoplasts, septate hyphae, thallic, holothallic, and holoblastic conidia, was greater, although olive-green septate hyphae with vesicular conidiogenous apparatus occurred at all K(+) levels tested.This study suggested that following the establishment of transmembrane pH gradient across protoplast membrane, operation of Mitchellian proton pump was further promoted, thus leading to active transport mechanism, a prelude to yeast morphology induction.

View Article: PubMed Central - PubMed

Affiliation: Department of Crop Science, Ambrose Alli University, Ekpoma, Nigeria.

ABSTRACT
Mucor circinelloides aerobically exhibits coenocytic thallic growth habit with straight and circinate sporangiophores which culminate in globose or pyriform columellae enclosed within sporangial walls. It undergoes dimorphic switch with its conversion to multipolar budding yeast-like cells or thallic conidia. This paper confirms the induction of plurality of reproductive structures of the pleomorphic microorganism in minimal medium. Furthermore, construction of pH differentials at inflection points in the biphasic profiles during sporangiospore-yeast transformation indicated the intensity of H(+) release from intracellular medium of the growing microorganism in a study conducted with K(+) levels (0.0, 0.5, 0.7, 0.9, 1.0,1.10 g/L)-mediated broths. Optimum proton release was at 0.00 and 1.0 g/L K(+)-supplemented broths, but specific growth rate was least in the latter. It also coincided with a preponderance of neoplastic units, protoplasts, and terminal budding yeast cells. On either side of this K(+) level, variation in morphologies, including neoplasts, protoplasts, septate hyphae, thallic, holothallic, and holoblastic conidia, was greater, although olive-green septate hyphae with vesicular conidiogenous apparatus occurred at all K(+) levels tested. This study suggested that following the establishment of transmembrane pH gradient across protoplast membrane, operation of Mitchellian proton pump was further promoted, thus leading to active transport mechanism, a prelude to yeast morphology induction.

No MeSH data available.


Related in: MedlinePlus