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Effect of Potassium Ions on Protoplast Generation during Yeast Induction from Mucor circinelloides Tieghem.

Omoifo CO - ISRN Biotechnol (2012)

Bottom Line: This paper confirms the induction of plurality of reproductive structures of the pleomorphic microorganism in minimal medium.On either side of this K(+) level, variation in morphologies, including neoplasts, protoplasts, septate hyphae, thallic, holothallic, and holoblastic conidia, was greater, although olive-green septate hyphae with vesicular conidiogenous apparatus occurred at all K(+) levels tested.This study suggested that following the establishment of transmembrane pH gradient across protoplast membrane, operation of Mitchellian proton pump was further promoted, thus leading to active transport mechanism, a prelude to yeast morphology induction.

View Article: PubMed Central - PubMed

Affiliation: Department of Crop Science, Ambrose Alli University, Ekpoma, Nigeria.

ABSTRACT
Mucor circinelloides aerobically exhibits coenocytic thallic growth habit with straight and circinate sporangiophores which culminate in globose or pyriform columellae enclosed within sporangial walls. It undergoes dimorphic switch with its conversion to multipolar budding yeast-like cells or thallic conidia. This paper confirms the induction of plurality of reproductive structures of the pleomorphic microorganism in minimal medium. Furthermore, construction of pH differentials at inflection points in the biphasic profiles during sporangiospore-yeast transformation indicated the intensity of H(+) release from intracellular medium of the growing microorganism in a study conducted with K(+) levels (0.0, 0.5, 0.7, 0.9, 1.0,1.10 g/L)-mediated broths. Optimum proton release was at 0.00 and 1.0 g/L K(+)-supplemented broths, but specific growth rate was least in the latter. It also coincided with a preponderance of neoplastic units, protoplasts, and terminal budding yeast cells. On either side of this K(+) level, variation in morphologies, including neoplasts, protoplasts, septate hyphae, thallic, holothallic, and holoblastic conidia, was greater, although olive-green septate hyphae with vesicular conidiogenous apparatus occurred at all K(+) levels tested. This study suggested that following the establishment of transmembrane pH gradient across protoplast membrane, operation of Mitchellian proton pump was further promoted, thus leading to active transport mechanism, a prelude to yeast morphology induction.

No MeSH data available.


Related in: MedlinePlus

Growth profiles of M. circinelloides Tieghem cultivated in K+-mediated glucose-substrate multi-ionic broth for 120 h at pH4.5, temp. 20°C, ambient.
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Related In: Results  -  Collection


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fig3: Growth profiles of M. circinelloides Tieghem cultivated in K+-mediated glucose-substrate multi-ionic broth for 120 h at pH4.5, temp. 20°C, ambient.

Mentions: Growth profiles of M. circinelloides, as affected by variation in K+ concentrations, were shown in Figure 3. Time-course study in control experiments gave more or less steady rise in OD reading but sigmoid growth pattern was not described (Figure 3(a)). Variation occurred in the growth profiles at the different K+ levels, but quite like the control experiments, none assumed sigmoid growth pattern (Figures 3(b)–3(f)). The log transformed OD reading was maximal after 48 h, in the 0.5 g/L and 1.10 g/ L broths, and thereafter fell to a level bellow that, or at par with the control tests, which was, in turn, less than those determined for the 0.7 and 0.9 g/L  K+ broths. Final OD determination in the control tests was however higher than the final value obtained at 1.0 g/L K+.


Effect of Potassium Ions on Protoplast Generation during Yeast Induction from Mucor circinelloides Tieghem.

Omoifo CO - ISRN Biotechnol (2012)

Growth profiles of M. circinelloides Tieghem cultivated in K+-mediated glucose-substrate multi-ionic broth for 120 h at pH4.5, temp. 20°C, ambient.
© Copyright Policy - open-access
Related In: Results  -  Collection

Show All Figures
getmorefigures.php?uid=PMC4403624&req=5

fig3: Growth profiles of M. circinelloides Tieghem cultivated in K+-mediated glucose-substrate multi-ionic broth for 120 h at pH4.5, temp. 20°C, ambient.
Mentions: Growth profiles of M. circinelloides, as affected by variation in K+ concentrations, were shown in Figure 3. Time-course study in control experiments gave more or less steady rise in OD reading but sigmoid growth pattern was not described (Figure 3(a)). Variation occurred in the growth profiles at the different K+ levels, but quite like the control experiments, none assumed sigmoid growth pattern (Figures 3(b)–3(f)). The log transformed OD reading was maximal after 48 h, in the 0.5 g/L and 1.10 g/ L broths, and thereafter fell to a level bellow that, or at par with the control tests, which was, in turn, less than those determined for the 0.7 and 0.9 g/L  K+ broths. Final OD determination in the control tests was however higher than the final value obtained at 1.0 g/L K+.

Bottom Line: This paper confirms the induction of plurality of reproductive structures of the pleomorphic microorganism in minimal medium.On either side of this K(+) level, variation in morphologies, including neoplasts, protoplasts, septate hyphae, thallic, holothallic, and holoblastic conidia, was greater, although olive-green septate hyphae with vesicular conidiogenous apparatus occurred at all K(+) levels tested.This study suggested that following the establishment of transmembrane pH gradient across protoplast membrane, operation of Mitchellian proton pump was further promoted, thus leading to active transport mechanism, a prelude to yeast morphology induction.

View Article: PubMed Central - PubMed

Affiliation: Department of Crop Science, Ambrose Alli University, Ekpoma, Nigeria.

ABSTRACT
Mucor circinelloides aerobically exhibits coenocytic thallic growth habit with straight and circinate sporangiophores which culminate in globose or pyriform columellae enclosed within sporangial walls. It undergoes dimorphic switch with its conversion to multipolar budding yeast-like cells or thallic conidia. This paper confirms the induction of plurality of reproductive structures of the pleomorphic microorganism in minimal medium. Furthermore, construction of pH differentials at inflection points in the biphasic profiles during sporangiospore-yeast transformation indicated the intensity of H(+) release from intracellular medium of the growing microorganism in a study conducted with K(+) levels (0.0, 0.5, 0.7, 0.9, 1.0,1.10 g/L)-mediated broths. Optimum proton release was at 0.00 and 1.0 g/L K(+)-supplemented broths, but specific growth rate was least in the latter. It also coincided with a preponderance of neoplastic units, protoplasts, and terminal budding yeast cells. On either side of this K(+) level, variation in morphologies, including neoplasts, protoplasts, septate hyphae, thallic, holothallic, and holoblastic conidia, was greater, although olive-green septate hyphae with vesicular conidiogenous apparatus occurred at all K(+) levels tested. This study suggested that following the establishment of transmembrane pH gradient across protoplast membrane, operation of Mitchellian proton pump was further promoted, thus leading to active transport mechanism, a prelude to yeast morphology induction.

No MeSH data available.


Related in: MedlinePlus