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Occurrence of Biosurfactant Producing Bacillus spp. in Diverse Habitats.

Joshi SJ, Suthar H, Yadav AK, Hingurao K, Nerurkar A - ISRN Biotechnol (2012)

Bottom Line: Present work revealed that diversity exists in distribution of Bacillus spp. from thermal and hydrocarbon containing habitats where majority of organisms belonged to B. licheniformis and B. subtilis group.This isolate clustered with B. subtilis and B. licheniformis group on the basis of ARDRA.These findings increase the possibility of exploiting the Bacillus spp. from different habitats and their possible use in oil recovery.

View Article: PubMed Central - PubMed

Affiliation: Department of Microbiology and Biotechnology Centre, Faculty of Science, The Maharaja Sayajirao University of Baroda, Vadodara 390002, Gujarat, India.

ABSTRACT
Diversity among biosurfactant producing Bacillus spp. from diverse habitats was studied among 77 isolates. Cluster analysis based on phenotypic characteristics using unweighted pair-group method with arithmetic averages (UPGMAs) method was performed. Bacillus isolates possessing high surface tension activity and five reference strains were subjected to amplified 16S rDNA restriction analysis (ARDRA). A correlation between the phenotypic and genotypic characterization of Bacillus spp. is explored. Most of the oil reservoir isolates showing high surface activity clustered with B. licheniformis and B. subtilis, the hot water spring isolates clustered in two ingroups, while the petroleum contaminated soil isolates were randomly distributed in all the three ingroups. Present work revealed that diversity exists in distribution of Bacillus spp. from thermal and hydrocarbon containing habitats where majority of organisms belonged to B. licheniformis and B. subtilis group. Isolate B. licheniformis TT42 produced biosurfactant which reduced the surface tension of water from 72 mNm(-1) to 28 mNm(-1), and 0.05 mNm(-1) interfacial tension against crude oil at 80°C. This isolate clustered with B. subtilis and B. licheniformis group on the basis of ARDRA. These findings increase the possibility of exploiting the Bacillus spp. from different habitats and their possible use in oil recovery.

No MeSH data available.


Related in: MedlinePlus

Dendrogram of genetic divergences among selected Bacillus isolates possessing high surface activity representing main phenetic groups and reference strains, based on restriction pattern obtained with Hha I, Msp Iand Hae III. BS: B. subtilis, BP: B. pumilus, BC: B. cereus, BM: B. megaterium and BL: B. licheniformis, Isolates TT42, TT21 and HTO.
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fig1: Dendrogram of genetic divergences among selected Bacillus isolates possessing high surface activity representing main phenetic groups and reference strains, based on restriction pattern obtained with Hha I, Msp Iand Hae III. BS: B. subtilis, BP: B. pumilus, BC: B. cereus, BM: B. megaterium and BL: B. licheniformis, Isolates TT42, TT21 and HTO.

Mentions: Three biosurfactant producing isolates, TT42 and TT21 belonging to the ingroup II and HTO representing ingroup III showing surface activity between 28–35 mNm−1 (Table 3) and five reference strains B. licheniformis, B. subtilis, B. cereus, B. pumilus, and B. megaterium were subjected to ARDRA by restriction enzymes Hae III, Hha I, and Msp I, and dendrogram based on restriction patterns of these three restriction enzymes was constructed (Figure 1).


Occurrence of Biosurfactant Producing Bacillus spp. in Diverse Habitats.

Joshi SJ, Suthar H, Yadav AK, Hingurao K, Nerurkar A - ISRN Biotechnol (2012)

Dendrogram of genetic divergences among selected Bacillus isolates possessing high surface activity representing main phenetic groups and reference strains, based on restriction pattern obtained with Hha I, Msp Iand Hae III. BS: B. subtilis, BP: B. pumilus, BC: B. cereus, BM: B. megaterium and BL: B. licheniformis, Isolates TT42, TT21 and HTO.
© Copyright Policy - open-access
Related In: Results  -  Collection

Show All Figures
getmorefigures.php?uid=PMC4403617&req=5

fig1: Dendrogram of genetic divergences among selected Bacillus isolates possessing high surface activity representing main phenetic groups and reference strains, based on restriction pattern obtained with Hha I, Msp Iand Hae III. BS: B. subtilis, BP: B. pumilus, BC: B. cereus, BM: B. megaterium and BL: B. licheniformis, Isolates TT42, TT21 and HTO.
Mentions: Three biosurfactant producing isolates, TT42 and TT21 belonging to the ingroup II and HTO representing ingroup III showing surface activity between 28–35 mNm−1 (Table 3) and five reference strains B. licheniformis, B. subtilis, B. cereus, B. pumilus, and B. megaterium were subjected to ARDRA by restriction enzymes Hae III, Hha I, and Msp I, and dendrogram based on restriction patterns of these three restriction enzymes was constructed (Figure 1).

Bottom Line: Present work revealed that diversity exists in distribution of Bacillus spp. from thermal and hydrocarbon containing habitats where majority of organisms belonged to B. licheniformis and B. subtilis group.This isolate clustered with B. subtilis and B. licheniformis group on the basis of ARDRA.These findings increase the possibility of exploiting the Bacillus spp. from different habitats and their possible use in oil recovery.

View Article: PubMed Central - PubMed

Affiliation: Department of Microbiology and Biotechnology Centre, Faculty of Science, The Maharaja Sayajirao University of Baroda, Vadodara 390002, Gujarat, India.

ABSTRACT
Diversity among biosurfactant producing Bacillus spp. from diverse habitats was studied among 77 isolates. Cluster analysis based on phenotypic characteristics using unweighted pair-group method with arithmetic averages (UPGMAs) method was performed. Bacillus isolates possessing high surface tension activity and five reference strains were subjected to amplified 16S rDNA restriction analysis (ARDRA). A correlation between the phenotypic and genotypic characterization of Bacillus spp. is explored. Most of the oil reservoir isolates showing high surface activity clustered with B. licheniformis and B. subtilis, the hot water spring isolates clustered in two ingroups, while the petroleum contaminated soil isolates were randomly distributed in all the three ingroups. Present work revealed that diversity exists in distribution of Bacillus spp. from thermal and hydrocarbon containing habitats where majority of organisms belonged to B. licheniformis and B. subtilis group. Isolate B. licheniformis TT42 produced biosurfactant which reduced the surface tension of water from 72 mNm(-1) to 28 mNm(-1), and 0.05 mNm(-1) interfacial tension against crude oil at 80°C. This isolate clustered with B. subtilis and B. licheniformis group on the basis of ARDRA. These findings increase the possibility of exploiting the Bacillus spp. from different habitats and their possible use in oil recovery.

No MeSH data available.


Related in: MedlinePlus