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The Use of Response Surface Methodology as a Statistical Tool for Media Optimization in Lipase Production from the Dairy Effluent Isolate Fusarium solani.

Kanmani P, Karthik S, Aravind J, Kumaresan K - ISRN Biotechnol (2012)

Bottom Line: A mesophilic temperature of 40°C, alkaline pH of 8, and incubation period of 72 hours were found to be the optimal conditions for lipase production.The optimum concentrations of three significant factors, namely, palm oil, (NH4)2SO4, and CaCO3 were determined by this method to be 5% (v/v), 5.5 g/L, and 0.1 g/L, respectively.RSM-guided design of experiments resulted in a maximum lipase production of 73.3 U/ml, which is a 1.7-fold increase in comparison with that obtained in the unoptimized medium.

View Article: PubMed Central - PubMed

Affiliation: Department of Biotechnology, Kumaraguru College of Technology, Coimbatore 641049, India.

ABSTRACT
The optimization of extracellular lipase production by Fusarium isolani strain SKWF7 isolated from dairy wastewater was carried out in this study. Initially, the physicochemical factors significantly influencing enzyme production were studied by varying one-factor-at-a-time (OFAT). A mesophilic temperature of 40°C, alkaline pH of 8, and incubation period of 72 hours were found to be the optimal conditions for lipase production. Among the media components, the disaccharide sucrose acted as the best carbon source; palm oil as the best inducing lipid substrate; casein and (NH4)2SO4 as the best organic and inorganic nitrogen sources; Ca(2+) ion as the best trace element. In the next phase of work, statistical optimization of medium components was performed by employing the Box-Behnken design of Response Surface Methodology (RSM). The optimum concentrations of three significant factors, namely, palm oil, (NH4)2SO4, and CaCO3 were determined by this method to be 5% (v/v), 5.5 g/L, and 0.1 g/L, respectively. RSM-guided design of experiments resulted in a maximum lipase production of 73.3 U/ml, which is a 1.7-fold increase in comparison with that obtained in the unoptimized medium. These results point towards the success of the model in developing a process for the production of lipase, an enzyme of enormous industrial significance.

No MeSH data available.


Related in: MedlinePlus

Effect of incubation time on lipase production.
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fig2: Effect of incubation time on lipase production.

Mentions: Enzyme production steadily increased with increasing incubation time of the culture and reached a plateau at 72 hours (Figure 2). Similar or longer incubation periods have been widely reported for fungi [19]. In the case of Mucor sp., highest production was obtained only after 6 days of cultivation [20]. Highest enzyme production was recorded with an incubation temperature of 40°C (Figure 3). Upon altering the culture medium pH, it was witnessed that there was good production in the range of 4.0–8.0, with the production peaking at 8 and rapidly falling thereafter (Figure 4). A strain of Aspergillus niger showed maximum enzyme production after 96 h of growth in a medium with an initial pH of 7.0 [21].


The Use of Response Surface Methodology as a Statistical Tool for Media Optimization in Lipase Production from the Dairy Effluent Isolate Fusarium solani.

Kanmani P, Karthik S, Aravind J, Kumaresan K - ISRN Biotechnol (2012)

Effect of incubation time on lipase production.
© Copyright Policy - open-access
Related In: Results  -  Collection

Show All Figures
getmorefigures.php?uid=PMC4403615&req=5

fig2: Effect of incubation time on lipase production.
Mentions: Enzyme production steadily increased with increasing incubation time of the culture and reached a plateau at 72 hours (Figure 2). Similar or longer incubation periods have been widely reported for fungi [19]. In the case of Mucor sp., highest production was obtained only after 6 days of cultivation [20]. Highest enzyme production was recorded with an incubation temperature of 40°C (Figure 3). Upon altering the culture medium pH, it was witnessed that there was good production in the range of 4.0–8.0, with the production peaking at 8 and rapidly falling thereafter (Figure 4). A strain of Aspergillus niger showed maximum enzyme production after 96 h of growth in a medium with an initial pH of 7.0 [21].

Bottom Line: A mesophilic temperature of 40°C, alkaline pH of 8, and incubation period of 72 hours were found to be the optimal conditions for lipase production.The optimum concentrations of three significant factors, namely, palm oil, (NH4)2SO4, and CaCO3 were determined by this method to be 5% (v/v), 5.5 g/L, and 0.1 g/L, respectively.RSM-guided design of experiments resulted in a maximum lipase production of 73.3 U/ml, which is a 1.7-fold increase in comparison with that obtained in the unoptimized medium.

View Article: PubMed Central - PubMed

Affiliation: Department of Biotechnology, Kumaraguru College of Technology, Coimbatore 641049, India.

ABSTRACT
The optimization of extracellular lipase production by Fusarium isolani strain SKWF7 isolated from dairy wastewater was carried out in this study. Initially, the physicochemical factors significantly influencing enzyme production were studied by varying one-factor-at-a-time (OFAT). A mesophilic temperature of 40°C, alkaline pH of 8, and incubation period of 72 hours were found to be the optimal conditions for lipase production. Among the media components, the disaccharide sucrose acted as the best carbon source; palm oil as the best inducing lipid substrate; casein and (NH4)2SO4 as the best organic and inorganic nitrogen sources; Ca(2+) ion as the best trace element. In the next phase of work, statistical optimization of medium components was performed by employing the Box-Behnken design of Response Surface Methodology (RSM). The optimum concentrations of three significant factors, namely, palm oil, (NH4)2SO4, and CaCO3 were determined by this method to be 5% (v/v), 5.5 g/L, and 0.1 g/L, respectively. RSM-guided design of experiments resulted in a maximum lipase production of 73.3 U/ml, which is a 1.7-fold increase in comparison with that obtained in the unoptimized medium. These results point towards the success of the model in developing a process for the production of lipase, an enzyme of enormous industrial significance.

No MeSH data available.


Related in: MedlinePlus