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Antimicrobial Pressure of Ciprofloxacin and Gentamicin on Biofilm Development by an Endoscope-Isolated Pseudomonas aeruginosa.

Machado I, Graça J, Lopes H, Lopes S, Pereira MO - ISRN Biotechnol (2012)

Bottom Line: The environmental pressure exerted by CIP and GM did not develop P. aeruginosa resistance to antibiotics nor cross-resistance towards BC.However, data highlighted that none of the antimicrobials led to complete biofilm eradication, allowing the recovery of the remaining adhered population possibly due to the selection of persister cells.This feature may lead to biofilm recalcitrance, reinforcement of bacterial attachment, and recolonization of other sites.

View Article: PubMed Central - PubMed

Affiliation: Centre of Biological Engineering, Institute for Biotechnology and Bioengineering (IBB), University of Minho, Campus de Gualtar, 4710-057 Braga, Portugal.

ABSTRACT
This work aims at characterizing endoscope biofilm-isolated (PAI) and reference strain P. aeruginosa (PA) adhesion, biofilm formation and sensitivity to antibiotics. The recovery ability of the biofilm-growing bacteria subjected to intermittent antibiotic pressure (ciprofloxacin (CIP) and gentamicin (GM)), as well as the development of resistance towards antibiotics and benzalkonium chloride (BC), were also determined. The capacity of both strains to develop biofilms was greatly impaired in the presence of CIP and GM. Sanitization was not complete allowing biofilm recovery after the intermittent cycles of antibiotic pressure. The environmental pressure exerted by CIP and GM did not develop P. aeruginosa resistance to antibiotics nor cross-resistance towards BC. However, data highlighted that none of the antimicrobials led to complete biofilm eradication, allowing the recovery of the remaining adhered population possibly due to the selection of persister cells. This feature may lead to biofilm recalcitrance, reinforcement of bacterial attachment, and recolonization of other sites.

No MeSH data available.


Related in: MedlinePlus

Biomass (OD570 nm) (a), metabolic activity (OD490 nm) (b) and number of cultivable cells (c) of P. aeruginosa ATCC (light grey) and P. aeruginosa isolated strain (black) biofilms. Biofilms where grown in TSB and characterized at 24, 48, 72 and 96 h. Bars represent the average of 3 independent repeats ± SD.
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fig1: Biomass (OD570 nm) (a), metabolic activity (OD490 nm) (b) and number of cultivable cells (c) of P. aeruginosa ATCC (light grey) and P. aeruginosa isolated strain (black) biofilms. Biofilms where grown in TSB and characterized at 24, 48, 72 and 96 h. Bars represent the average of 3 independent repeats ± SD.

Mentions: In order to examine the biofilm formation ability of both PAI and PA strains, the biofilm phenotype was characterized in terms of mass, activity and number of cells after 24, 48, 72, and 96 h of growth (Figure 1). In general, data showed that mass and activity of biofilms increased along time, whereas the number of biofilm-entrapped cells was approximately in the same magnitude, for all the time periods of biofilm formation. Comparing both strains, Figure 1 shows that, in general, PAI gave rise to biofilms with more mass than PA (P < 0.05) and activity (P < 0.05). The number of biofilm cells (Figure 1(c)) was identical to those quantified for PA biofilms, except for 72-hour-old and 96-h-old biofilms in which there was an increase in the number of cells (P < 0.001). These results indicate that both strains are good biofilm producers although the isolate stands out relatively to the collection.


Antimicrobial Pressure of Ciprofloxacin and Gentamicin on Biofilm Development by an Endoscope-Isolated Pseudomonas aeruginosa.

Machado I, Graça J, Lopes H, Lopes S, Pereira MO - ISRN Biotechnol (2012)

Biomass (OD570 nm) (a), metabolic activity (OD490 nm) (b) and number of cultivable cells (c) of P. aeruginosa ATCC (light grey) and P. aeruginosa isolated strain (black) biofilms. Biofilms where grown in TSB and characterized at 24, 48, 72 and 96 h. Bars represent the average of 3 independent repeats ± SD.
© Copyright Policy - open-access
Related In: Results  -  Collection

Show All Figures
getmorefigures.php?uid=PMC4403614&req=5

fig1: Biomass (OD570 nm) (a), metabolic activity (OD490 nm) (b) and number of cultivable cells (c) of P. aeruginosa ATCC (light grey) and P. aeruginosa isolated strain (black) biofilms. Biofilms where grown in TSB and characterized at 24, 48, 72 and 96 h. Bars represent the average of 3 independent repeats ± SD.
Mentions: In order to examine the biofilm formation ability of both PAI and PA strains, the biofilm phenotype was characterized in terms of mass, activity and number of cells after 24, 48, 72, and 96 h of growth (Figure 1). In general, data showed that mass and activity of biofilms increased along time, whereas the number of biofilm-entrapped cells was approximately in the same magnitude, for all the time periods of biofilm formation. Comparing both strains, Figure 1 shows that, in general, PAI gave rise to biofilms with more mass than PA (P < 0.05) and activity (P < 0.05). The number of biofilm cells (Figure 1(c)) was identical to those quantified for PA biofilms, except for 72-hour-old and 96-h-old biofilms in which there was an increase in the number of cells (P < 0.001). These results indicate that both strains are good biofilm producers although the isolate stands out relatively to the collection.

Bottom Line: The environmental pressure exerted by CIP and GM did not develop P. aeruginosa resistance to antibiotics nor cross-resistance towards BC.However, data highlighted that none of the antimicrobials led to complete biofilm eradication, allowing the recovery of the remaining adhered population possibly due to the selection of persister cells.This feature may lead to biofilm recalcitrance, reinforcement of bacterial attachment, and recolonization of other sites.

View Article: PubMed Central - PubMed

Affiliation: Centre of Biological Engineering, Institute for Biotechnology and Bioengineering (IBB), University of Minho, Campus de Gualtar, 4710-057 Braga, Portugal.

ABSTRACT
This work aims at characterizing endoscope biofilm-isolated (PAI) and reference strain P. aeruginosa (PA) adhesion, biofilm formation and sensitivity to antibiotics. The recovery ability of the biofilm-growing bacteria subjected to intermittent antibiotic pressure (ciprofloxacin (CIP) and gentamicin (GM)), as well as the development of resistance towards antibiotics and benzalkonium chloride (BC), were also determined. The capacity of both strains to develop biofilms was greatly impaired in the presence of CIP and GM. Sanitization was not complete allowing biofilm recovery after the intermittent cycles of antibiotic pressure. The environmental pressure exerted by CIP and GM did not develop P. aeruginosa resistance to antibiotics nor cross-resistance towards BC. However, data highlighted that none of the antimicrobials led to complete biofilm eradication, allowing the recovery of the remaining adhered population possibly due to the selection of persister cells. This feature may lead to biofilm recalcitrance, reinforcement of bacterial attachment, and recolonization of other sites.

No MeSH data available.


Related in: MedlinePlus