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Challenges in enzymatic route of mannitol production.

Bhatt SM, Mohan A, Srivastava SK - ISRN Biotechnol (2012)

Bottom Line: Mannitol is an important biochemical often used as medicine and in food sector, yet its biotechnological is not preffered in Industry for large scale production, which may be due to the multistep mechanism involved in hydrogenation and reduction.This paper is a comparative preview covering present chemical and biotechnological approaches existing today for mannitol production at industrial scale.Biotechnological routes are suitable for adaptation at industrial level for mannitol production, and whatever concerns are there had been discussed in detail, namely, raw materials, broad range of enzymes with high activity at elevated temperature suitable for use in reactor, cofactor limitation, reduced by-product formation, end product inhibition, and reduced utilization of mannitol for enhancing the yield with maximum volumetric productivity.

View Article: PubMed Central - PubMed

Affiliation: Biotechnology Department, Lovely Professional University, Punjab 144 401, India.

ABSTRACT
Mannitol is an important biochemical often used as medicine and in food sector, yet its biotechnological is not preffered in Industry for large scale production, which may be due to the multistep mechanism involved in hydrogenation and reduction. This paper is a comparative preview covering present chemical and biotechnological approaches existing today for mannitol production at industrial scale. Biotechnological routes are suitable for adaptation at industrial level for mannitol production, and whatever concerns are there had been discussed in detail, namely, raw materials, broad range of enzymes with high activity at elevated temperature suitable for use in reactor, cofactor limitation, reduced by-product formation, end product inhibition, and reduced utilization of mannitol for enhancing the yield with maximum volumetric productivity.

No MeSH data available.


(a) Km Values for the enzymes 1.1.1.67 (b) Km Values for the enzymes 1.1.1.138 (source Brenda data base).
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fig6: (a) Km Values for the enzymes 1.1.1.67 (b) Km Values for the enzymes 1.1.1.138 (source Brenda data base).

Mentions: From BRENDA enzyme data base server, a plot (Figure 6) was obtained between Km versus number of bacteria shows range of Km and also has been depicted in Table 3 varying from 0.44 to 71 mm for fructose [52] and for mannitol from 0.29 to 78 mm [16, 52, 63]. From database following type of MDH reported to exist (1) Mannitol 1-phosphate dehydrogenase EC 1.1.1.17 (mtlD); (2) The NADPH-dependent MDH (EC 1.1.1.138) and (3) EC 1.1.1.67, encoded by mtlK from L. intermedius (NRRL B-3693). Most of the enzyme was having the higher Km for fructose which is the main cause of lower production of mannitol [5].


Challenges in enzymatic route of mannitol production.

Bhatt SM, Mohan A, Srivastava SK - ISRN Biotechnol (2012)

(a) Km Values for the enzymes 1.1.1.67 (b) Km Values for the enzymes 1.1.1.138 (source Brenda data base).
© Copyright Policy - open-access
Related In: Results  -  Collection

Show All Figures
getmorefigures.php?uid=PMC4403613&req=5

fig6: (a) Km Values for the enzymes 1.1.1.67 (b) Km Values for the enzymes 1.1.1.138 (source Brenda data base).
Mentions: From BRENDA enzyme data base server, a plot (Figure 6) was obtained between Km versus number of bacteria shows range of Km and also has been depicted in Table 3 varying from 0.44 to 71 mm for fructose [52] and for mannitol from 0.29 to 78 mm [16, 52, 63]. From database following type of MDH reported to exist (1) Mannitol 1-phosphate dehydrogenase EC 1.1.1.17 (mtlD); (2) The NADPH-dependent MDH (EC 1.1.1.138) and (3) EC 1.1.1.67, encoded by mtlK from L. intermedius (NRRL B-3693). Most of the enzyme was having the higher Km for fructose which is the main cause of lower production of mannitol [5].

Bottom Line: Mannitol is an important biochemical often used as medicine and in food sector, yet its biotechnological is not preffered in Industry for large scale production, which may be due to the multistep mechanism involved in hydrogenation and reduction.This paper is a comparative preview covering present chemical and biotechnological approaches existing today for mannitol production at industrial scale.Biotechnological routes are suitable for adaptation at industrial level for mannitol production, and whatever concerns are there had been discussed in detail, namely, raw materials, broad range of enzymes with high activity at elevated temperature suitable for use in reactor, cofactor limitation, reduced by-product formation, end product inhibition, and reduced utilization of mannitol for enhancing the yield with maximum volumetric productivity.

View Article: PubMed Central - PubMed

Affiliation: Biotechnology Department, Lovely Professional University, Punjab 144 401, India.

ABSTRACT
Mannitol is an important biochemical often used as medicine and in food sector, yet its biotechnological is not preffered in Industry for large scale production, which may be due to the multistep mechanism involved in hydrogenation and reduction. This paper is a comparative preview covering present chemical and biotechnological approaches existing today for mannitol production at industrial scale. Biotechnological routes are suitable for adaptation at industrial level for mannitol production, and whatever concerns are there had been discussed in detail, namely, raw materials, broad range of enzymes with high activity at elevated temperature suitable for use in reactor, cofactor limitation, reduced by-product formation, end product inhibition, and reduced utilization of mannitol for enhancing the yield with maximum volumetric productivity.

No MeSH data available.