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Challenges in enzymatic route of mannitol production.

Bhatt SM, Mohan A, Srivastava SK - ISRN Biotechnol (2012)

Bottom Line: Mannitol is an important biochemical often used as medicine and in food sector, yet its biotechnological is not preffered in Industry for large scale production, which may be due to the multistep mechanism involved in hydrogenation and reduction.This paper is a comparative preview covering present chemical and biotechnological approaches existing today for mannitol production at industrial scale.Biotechnological routes are suitable for adaptation at industrial level for mannitol production, and whatever concerns are there had been discussed in detail, namely, raw materials, broad range of enzymes with high activity at elevated temperature suitable for use in reactor, cofactor limitation, reduced by-product formation, end product inhibition, and reduced utilization of mannitol for enhancing the yield with maximum volumetric productivity.

View Article: PubMed Central - PubMed

Affiliation: Biotechnology Department, Lovely Professional University, Punjab 144 401, India.

ABSTRACT
Mannitol is an important biochemical often used as medicine and in food sector, yet its biotechnological is not preffered in Industry for large scale production, which may be due to the multistep mechanism involved in hydrogenation and reduction. This paper is a comparative preview covering present chemical and biotechnological approaches existing today for mannitol production at industrial scale. Biotechnological routes are suitable for adaptation at industrial level for mannitol production, and whatever concerns are there had been discussed in detail, namely, raw materials, broad range of enzymes with high activity at elevated temperature suitable for use in reactor, cofactor limitation, reduced by-product formation, end product inhibition, and reduced utilization of mannitol for enhancing the yield with maximum volumetric productivity.

No MeSH data available.


Mechanism of action of mtlADR operon. The transcriptional activator MtlR, which in the presence of mannitol produces derepression of the mtlADR operon, but when glucose is present in the medium, this operon is a target of catabolite repression (through CRP), independent of MtlR. MtlA takes up exogenous mannitol, releasing the phosphate ester, mannitol-1-P, into the cell cytoplasm, but it is not clear which of the two metabolites is the inducer of MtlR http://biocyc.org/ECOLI/NEW-IMAGE?type=OPERON&object=TU00193.
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fig5: Mechanism of action of mtlADR operon. The transcriptional activator MtlR, which in the presence of mannitol produces derepression of the mtlADR operon, but when glucose is present in the medium, this operon is a target of catabolite repression (through CRP), independent of MtlR. MtlA takes up exogenous mannitol, releasing the phosphate ester, mannitol-1-P, into the cell cytoplasm, but it is not clear which of the two metabolites is the inducer of MtlR http://biocyc.org/ECOLI/NEW-IMAGE?type=OPERON&object=TU00193.

Mentions: Beside this, pH is also thought to play an important role in modulation of mannitol transporter protein. In L. lactis there exits the mannose-PTS system (PTSman) (Figure 5) [49], which, besides glucose, is reported to transport 2-deoxy-D-glucose, mannose, glucosamine, and fructose.


Challenges in enzymatic route of mannitol production.

Bhatt SM, Mohan A, Srivastava SK - ISRN Biotechnol (2012)

Mechanism of action of mtlADR operon. The transcriptional activator MtlR, which in the presence of mannitol produces derepression of the mtlADR operon, but when glucose is present in the medium, this operon is a target of catabolite repression (through CRP), independent of MtlR. MtlA takes up exogenous mannitol, releasing the phosphate ester, mannitol-1-P, into the cell cytoplasm, but it is not clear which of the two metabolites is the inducer of MtlR http://biocyc.org/ECOLI/NEW-IMAGE?type=OPERON&object=TU00193.
© Copyright Policy - open-access
Related In: Results  -  Collection

Show All Figures
getmorefigures.php?uid=PMC4403613&req=5

fig5: Mechanism of action of mtlADR operon. The transcriptional activator MtlR, which in the presence of mannitol produces derepression of the mtlADR operon, but when glucose is present in the medium, this operon is a target of catabolite repression (through CRP), independent of MtlR. MtlA takes up exogenous mannitol, releasing the phosphate ester, mannitol-1-P, into the cell cytoplasm, but it is not clear which of the two metabolites is the inducer of MtlR http://biocyc.org/ECOLI/NEW-IMAGE?type=OPERON&object=TU00193.
Mentions: Beside this, pH is also thought to play an important role in modulation of mannitol transporter protein. In L. lactis there exits the mannose-PTS system (PTSman) (Figure 5) [49], which, besides glucose, is reported to transport 2-deoxy-D-glucose, mannose, glucosamine, and fructose.

Bottom Line: Mannitol is an important biochemical often used as medicine and in food sector, yet its biotechnological is not preffered in Industry for large scale production, which may be due to the multistep mechanism involved in hydrogenation and reduction.This paper is a comparative preview covering present chemical and biotechnological approaches existing today for mannitol production at industrial scale.Biotechnological routes are suitable for adaptation at industrial level for mannitol production, and whatever concerns are there had been discussed in detail, namely, raw materials, broad range of enzymes with high activity at elevated temperature suitable for use in reactor, cofactor limitation, reduced by-product formation, end product inhibition, and reduced utilization of mannitol for enhancing the yield with maximum volumetric productivity.

View Article: PubMed Central - PubMed

Affiliation: Biotechnology Department, Lovely Professional University, Punjab 144 401, India.

ABSTRACT
Mannitol is an important biochemical often used as medicine and in food sector, yet its biotechnological is not preffered in Industry for large scale production, which may be due to the multistep mechanism involved in hydrogenation and reduction. This paper is a comparative preview covering present chemical and biotechnological approaches existing today for mannitol production at industrial scale. Biotechnological routes are suitable for adaptation at industrial level for mannitol production, and whatever concerns are there had been discussed in detail, namely, raw materials, broad range of enzymes with high activity at elevated temperature suitable for use in reactor, cofactor limitation, reduced by-product formation, end product inhibition, and reduced utilization of mannitol for enhancing the yield with maximum volumetric productivity.

No MeSH data available.