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Challenges in enzymatic route of mannitol production.

Bhatt SM, Mohan A, Srivastava SK - ISRN Biotechnol (2012)

Bottom Line: Mannitol is an important biochemical often used as medicine and in food sector, yet its biotechnological is not preffered in Industry for large scale production, which may be due to the multistep mechanism involved in hydrogenation and reduction.This paper is a comparative preview covering present chemical and biotechnological approaches existing today for mannitol production at industrial scale.Biotechnological routes are suitable for adaptation at industrial level for mannitol production, and whatever concerns are there had been discussed in detail, namely, raw materials, broad range of enzymes with high activity at elevated temperature suitable for use in reactor, cofactor limitation, reduced by-product formation, end product inhibition, and reduced utilization of mannitol for enhancing the yield with maximum volumetric productivity.

View Article: PubMed Central - PubMed

Affiliation: Biotechnology Department, Lovely Professional University, Punjab 144 401, India.

ABSTRACT
Mannitol is an important biochemical often used as medicine and in food sector, yet its biotechnological is not preffered in Industry for large scale production, which may be due to the multistep mechanism involved in hydrogenation and reduction. This paper is a comparative preview covering present chemical and biotechnological approaches existing today for mannitol production at industrial scale. Biotechnological routes are suitable for adaptation at industrial level for mannitol production, and whatever concerns are there had been discussed in detail, namely, raw materials, broad range of enzymes with high activity at elevated temperature suitable for use in reactor, cofactor limitation, reduced by-product formation, end product inhibition, and reduced utilization of mannitol for enhancing the yield with maximum volumetric productivity.

No MeSH data available.


Related in: MedlinePlus

Proposed sucrose metabolism in L. lactis NZ9800/NZ9841 overproducer strain MDH isolated from Leuconostoc mesenteroides. (a), phosphorylation of fructose into fructose 6-phosphate by fructokinase (b), export and the subsequent import of fructose by the fructose PTS (PTSfru) or the mannose PTS (PTSman) (c), and the conversion of fructose into glucose by a xylose isomerase-like enzyme activity (d). Mannitol is exported by an unknown mechanism, and is possibly re-utilized by the mannitol PTS (PTSmtl) [18]. (e) Mannitol uptake mechanism. Enzyme abbreviations: sucrose 6-phosphate hydrolase (S6PH), mannitol dehydrogenase (MDH), fructokinase (FK), glucokinase (GK), 1-phosphofructokinase (1-PFK), xylose isomerase (XI), phosphotransferase system (PTS).
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fig4: Proposed sucrose metabolism in L. lactis NZ9800/NZ9841 overproducer strain MDH isolated from Leuconostoc mesenteroides. (a), phosphorylation of fructose into fructose 6-phosphate by fructokinase (b), export and the subsequent import of fructose by the fructose PTS (PTSfru) or the mannose PTS (PTSman) (c), and the conversion of fructose into glucose by a xylose isomerase-like enzyme activity (d). Mannitol is exported by an unknown mechanism, and is possibly re-utilized by the mannitol PTS (PTSmtl) [18]. (e) Mannitol uptake mechanism. Enzyme abbreviations: sucrose 6-phosphate hydrolase (S6PH), mannitol dehydrogenase (MDH), fructokinase (FK), glucokinase (GK), 1-phosphofructokinase (1-PFK), xylose isomerase (XI), phosphotransferase system (PTS).

Mentions: Figure 4 depicts the transporter molecule (PTS) having role in transport of various substrate for metabolism. In homofermentative LAB (L. lactis), PTSman is reported to be the main transporter for glucose and fructose, which yields fructose 6-phosphate after phosphorylation.


Challenges in enzymatic route of mannitol production.

Bhatt SM, Mohan A, Srivastava SK - ISRN Biotechnol (2012)

Proposed sucrose metabolism in L. lactis NZ9800/NZ9841 overproducer strain MDH isolated from Leuconostoc mesenteroides. (a), phosphorylation of fructose into fructose 6-phosphate by fructokinase (b), export and the subsequent import of fructose by the fructose PTS (PTSfru) or the mannose PTS (PTSman) (c), and the conversion of fructose into glucose by a xylose isomerase-like enzyme activity (d). Mannitol is exported by an unknown mechanism, and is possibly re-utilized by the mannitol PTS (PTSmtl) [18]. (e) Mannitol uptake mechanism. Enzyme abbreviations: sucrose 6-phosphate hydrolase (S6PH), mannitol dehydrogenase (MDH), fructokinase (FK), glucokinase (GK), 1-phosphofructokinase (1-PFK), xylose isomerase (XI), phosphotransferase system (PTS).
© Copyright Policy - open-access
Related In: Results  -  Collection

Show All Figures
getmorefigures.php?uid=PMC4403613&req=5

fig4: Proposed sucrose metabolism in L. lactis NZ9800/NZ9841 overproducer strain MDH isolated from Leuconostoc mesenteroides. (a), phosphorylation of fructose into fructose 6-phosphate by fructokinase (b), export and the subsequent import of fructose by the fructose PTS (PTSfru) or the mannose PTS (PTSman) (c), and the conversion of fructose into glucose by a xylose isomerase-like enzyme activity (d). Mannitol is exported by an unknown mechanism, and is possibly re-utilized by the mannitol PTS (PTSmtl) [18]. (e) Mannitol uptake mechanism. Enzyme abbreviations: sucrose 6-phosphate hydrolase (S6PH), mannitol dehydrogenase (MDH), fructokinase (FK), glucokinase (GK), 1-phosphofructokinase (1-PFK), xylose isomerase (XI), phosphotransferase system (PTS).
Mentions: Figure 4 depicts the transporter molecule (PTS) having role in transport of various substrate for metabolism. In homofermentative LAB (L. lactis), PTSman is reported to be the main transporter for glucose and fructose, which yields fructose 6-phosphate after phosphorylation.

Bottom Line: Mannitol is an important biochemical often used as medicine and in food sector, yet its biotechnological is not preffered in Industry for large scale production, which may be due to the multistep mechanism involved in hydrogenation and reduction.This paper is a comparative preview covering present chemical and biotechnological approaches existing today for mannitol production at industrial scale.Biotechnological routes are suitable for adaptation at industrial level for mannitol production, and whatever concerns are there had been discussed in detail, namely, raw materials, broad range of enzymes with high activity at elevated temperature suitable for use in reactor, cofactor limitation, reduced by-product formation, end product inhibition, and reduced utilization of mannitol for enhancing the yield with maximum volumetric productivity.

View Article: PubMed Central - PubMed

Affiliation: Biotechnology Department, Lovely Professional University, Punjab 144 401, India.

ABSTRACT
Mannitol is an important biochemical often used as medicine and in food sector, yet its biotechnological is not preffered in Industry for large scale production, which may be due to the multistep mechanism involved in hydrogenation and reduction. This paper is a comparative preview covering present chemical and biotechnological approaches existing today for mannitol production at industrial scale. Biotechnological routes are suitable for adaptation at industrial level for mannitol production, and whatever concerns are there had been discussed in detail, namely, raw materials, broad range of enzymes with high activity at elevated temperature suitable for use in reactor, cofactor limitation, reduced by-product formation, end product inhibition, and reduced utilization of mannitol for enhancing the yield with maximum volumetric productivity.

No MeSH data available.


Related in: MedlinePlus