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Challenges in enzymatic route of mannitol production.

Bhatt SM, Mohan A, Srivastava SK - ISRN Biotechnol (2012)

Bottom Line: Mannitol is an important biochemical often used as medicine and in food sector, yet its biotechnological is not preffered in Industry for large scale production, which may be due to the multistep mechanism involved in hydrogenation and reduction.This paper is a comparative preview covering present chemical and biotechnological approaches existing today for mannitol production at industrial scale.Biotechnological routes are suitable for adaptation at industrial level for mannitol production, and whatever concerns are there had been discussed in detail, namely, raw materials, broad range of enzymes with high activity at elevated temperature suitable for use in reactor, cofactor limitation, reduced by-product formation, end product inhibition, and reduced utilization of mannitol for enhancing the yield with maximum volumetric productivity.

View Article: PubMed Central - PubMed

Affiliation: Biotechnology Department, Lovely Professional University, Punjab 144 401, India.

ABSTRACT
Mannitol is an important biochemical often used as medicine and in food sector, yet its biotechnological is not preffered in Industry for large scale production, which may be due to the multistep mechanism involved in hydrogenation and reduction. This paper is a comparative preview covering present chemical and biotechnological approaches existing today for mannitol production at industrial scale. Biotechnological routes are suitable for adaptation at industrial level for mannitol production, and whatever concerns are there had been discussed in detail, namely, raw materials, broad range of enzymes with high activity at elevated temperature suitable for use in reactor, cofactor limitation, reduced by-product formation, end product inhibition, and reduced utilization of mannitol for enhancing the yield with maximum volumetric productivity.

No MeSH data available.


Cofactor regeneration strategies in mannitol production.
© Copyright Policy - open-access
Related In: Results  -  Collection


getmorefigures.php?uid=PMC4403613&req=5

fig1: Cofactor regeneration strategies in mannitol production.

Mentions: The chief enzyme accounted for enzymatic hydrogenation of fructose into mannitol is NADPH-dependent mannitol dehydrogenase (mdh) (EC 1.1.1.67), but due to unavailability of a suitable regeneration method of NAD or NAD(P)H cofactor, again it was not employed practically. Therefore, a new strategys was adapted to regenerate the cofactor which was co-fermentation of the two substrates glucose and fructose (1 : 1), where glucose gets converted into gluconate yielding NADH by glucose dehydrogenase Howaldt et al. [11] (Figure 1).


Challenges in enzymatic route of mannitol production.

Bhatt SM, Mohan A, Srivastava SK - ISRN Biotechnol (2012)

Cofactor regeneration strategies in mannitol production.
© Copyright Policy - open-access
Related In: Results  -  Collection

Show All Figures
getmorefigures.php?uid=PMC4403613&req=5

fig1: Cofactor regeneration strategies in mannitol production.
Mentions: The chief enzyme accounted for enzymatic hydrogenation of fructose into mannitol is NADPH-dependent mannitol dehydrogenase (mdh) (EC 1.1.1.67), but due to unavailability of a suitable regeneration method of NAD or NAD(P)H cofactor, again it was not employed practically. Therefore, a new strategys was adapted to regenerate the cofactor which was co-fermentation of the two substrates glucose and fructose (1 : 1), where glucose gets converted into gluconate yielding NADH by glucose dehydrogenase Howaldt et al. [11] (Figure 1).

Bottom Line: Mannitol is an important biochemical often used as medicine and in food sector, yet its biotechnological is not preffered in Industry for large scale production, which may be due to the multistep mechanism involved in hydrogenation and reduction.This paper is a comparative preview covering present chemical and biotechnological approaches existing today for mannitol production at industrial scale.Biotechnological routes are suitable for adaptation at industrial level for mannitol production, and whatever concerns are there had been discussed in detail, namely, raw materials, broad range of enzymes with high activity at elevated temperature suitable for use in reactor, cofactor limitation, reduced by-product formation, end product inhibition, and reduced utilization of mannitol for enhancing the yield with maximum volumetric productivity.

View Article: PubMed Central - PubMed

Affiliation: Biotechnology Department, Lovely Professional University, Punjab 144 401, India.

ABSTRACT
Mannitol is an important biochemical often used as medicine and in food sector, yet its biotechnological is not preffered in Industry for large scale production, which may be due to the multistep mechanism involved in hydrogenation and reduction. This paper is a comparative preview covering present chemical and biotechnological approaches existing today for mannitol production at industrial scale. Biotechnological routes are suitable for adaptation at industrial level for mannitol production, and whatever concerns are there had been discussed in detail, namely, raw materials, broad range of enzymes with high activity at elevated temperature suitable for use in reactor, cofactor limitation, reduced by-product formation, end product inhibition, and reduced utilization of mannitol for enhancing the yield with maximum volumetric productivity.

No MeSH data available.