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Biosynthesis of Gold Nanoparticles Using Fusarium oxysporum f. sp. cubense JT1, a Plant Pathogenic Fungus.

Thakker JN, Dalwadi P, Dhandhukia PC - ISRN Biotechnol (2012)

Bottom Line: Recently, reports are published on the extracellular as well as intracellular biosynthesis of gold nanoparticles using microorganisms.Incubation of FocJT1 mycelium with auric chloride solution produces gold nanoparticles in 60 min.Gold nanoparticles were characterized by UV-Vis spectroscopy, FTIR, and particle size analysis.

View Article: PubMed Central - PubMed

Affiliation: Department of Biotechnology, P. D. Patel Institute of Applied Science, Charotar University of Science & Technology, Education Campus Changa, Changa 388421, India.

ABSTRACT
The development of reliable processes for the synthesis of gold nanoparticles is an important aspect of current nanotechnology research. Recently, reports are published on the extracellular as well as intracellular biosynthesis of gold nanoparticles using microorganisms. However, these methods of synthesis are rather slow. In present study, rapid and extracellular synthesis of gold nanoparticles using a plant pathogenic fungus F. oxysporum f. sp. cubense JT1 (FocJT1) is reported. Incubation of FocJT1 mycelium with auric chloride solution produces gold nanoparticles in 60 min. Gold nanoparticles were characterized by UV-Vis spectroscopy, FTIR, and particle size analysis. The particles synthesized were of 22 nm sized, capped by proteins, and posed antimicrobial activity against Pseudomonas sp.

No MeSH data available.


Related in: MedlinePlus

FTIR spectra of (a) dried mycelial mat and (b) mycelia mat incubated with 10 mM HAuCl4 solution. Mycelia mat collected are shown in inset.
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fig3: FTIR spectra of (a) dried mycelial mat and (b) mycelia mat incubated with 10 mM HAuCl4 solution. Mycelia mat collected are shown in inset.

Mentions: FTIR measurement carried out shows the amide bands, which were due to –N–H stretch and carbonyl stretch vibration in the amide linkages of the protein. The bands at 1634 and 1560 cm−1 were identified as the amide I and II bands [22] and arise due to carbonyl stretch and –N–H stretch vibrations in the amide linkages of the proteins, respectively [23]. The positions of these bands are close to those reported for native proteins. The FTIR results thus indicate that secondary structure of the proteins is not affected because of reaction with the Au3+ ions or binding with the gold nanoparticles. The band at ca. 1458 cm−1 is assigned to methylene scissoring vibrations from the proteins in the solution (Figure 3). On comparison of the IR spectra of FocJT1 control and experimental mat with gold nanoparticle, it was observed that 1654 and 1460 cm−1 bands were masked. This indicated that the gold particle formed were in conjugation with protein. Intensity of band 3385 cm−1 was reduced indicating that –OH stretching of alcohol and phenol present on the surface of the mycelial mat might be involved either in the formation or the attachment.


Biosynthesis of Gold Nanoparticles Using Fusarium oxysporum f. sp. cubense JT1, a Plant Pathogenic Fungus.

Thakker JN, Dalwadi P, Dhandhukia PC - ISRN Biotechnol (2012)

FTIR spectra of (a) dried mycelial mat and (b) mycelia mat incubated with 10 mM HAuCl4 solution. Mycelia mat collected are shown in inset.
© Copyright Policy - open-access
Related In: Results  -  Collection

Show All Figures
getmorefigures.php?uid=PMC4403611&req=5

fig3: FTIR spectra of (a) dried mycelial mat and (b) mycelia mat incubated with 10 mM HAuCl4 solution. Mycelia mat collected are shown in inset.
Mentions: FTIR measurement carried out shows the amide bands, which were due to –N–H stretch and carbonyl stretch vibration in the amide linkages of the protein. The bands at 1634 and 1560 cm−1 were identified as the amide I and II bands [22] and arise due to carbonyl stretch and –N–H stretch vibrations in the amide linkages of the proteins, respectively [23]. The positions of these bands are close to those reported for native proteins. The FTIR results thus indicate that secondary structure of the proteins is not affected because of reaction with the Au3+ ions or binding with the gold nanoparticles. The band at ca. 1458 cm−1 is assigned to methylene scissoring vibrations from the proteins in the solution (Figure 3). On comparison of the IR spectra of FocJT1 control and experimental mat with gold nanoparticle, it was observed that 1654 and 1460 cm−1 bands were masked. This indicated that the gold particle formed were in conjugation with protein. Intensity of band 3385 cm−1 was reduced indicating that –OH stretching of alcohol and phenol present on the surface of the mycelial mat might be involved either in the formation or the attachment.

Bottom Line: Recently, reports are published on the extracellular as well as intracellular biosynthesis of gold nanoparticles using microorganisms.Incubation of FocJT1 mycelium with auric chloride solution produces gold nanoparticles in 60 min.Gold nanoparticles were characterized by UV-Vis spectroscopy, FTIR, and particle size analysis.

View Article: PubMed Central - PubMed

Affiliation: Department of Biotechnology, P. D. Patel Institute of Applied Science, Charotar University of Science & Technology, Education Campus Changa, Changa 388421, India.

ABSTRACT
The development of reliable processes for the synthesis of gold nanoparticles is an important aspect of current nanotechnology research. Recently, reports are published on the extracellular as well as intracellular biosynthesis of gold nanoparticles using microorganisms. However, these methods of synthesis are rather slow. In present study, rapid and extracellular synthesis of gold nanoparticles using a plant pathogenic fungus F. oxysporum f. sp. cubense JT1 (FocJT1) is reported. Incubation of FocJT1 mycelium with auric chloride solution produces gold nanoparticles in 60 min. Gold nanoparticles were characterized by UV-Vis spectroscopy, FTIR, and particle size analysis. The particles synthesized were of 22 nm sized, capped by proteins, and posed antimicrobial activity against Pseudomonas sp.

No MeSH data available.


Related in: MedlinePlus