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Biosynthesis of Gold Nanoparticles Using Fusarium oxysporum f. sp. cubense JT1, a Plant Pathogenic Fungus.

Thakker JN, Dalwadi P, Dhandhukia PC - ISRN Biotechnol (2012)

Bottom Line: Recently, reports are published on the extracellular as well as intracellular biosynthesis of gold nanoparticles using microorganisms.Incubation of FocJT1 mycelium with auric chloride solution produces gold nanoparticles in 60 min.Gold nanoparticles were characterized by UV-Vis spectroscopy, FTIR, and particle size analysis.

View Article: PubMed Central - PubMed

Affiliation: Department of Biotechnology, P. D. Patel Institute of Applied Science, Charotar University of Science & Technology, Education Campus Changa, Changa 388421, India.

ABSTRACT
The development of reliable processes for the synthesis of gold nanoparticles is an important aspect of current nanotechnology research. Recently, reports are published on the extracellular as well as intracellular biosynthesis of gold nanoparticles using microorganisms. However, these methods of synthesis are rather slow. In present study, rapid and extracellular synthesis of gold nanoparticles using a plant pathogenic fungus F. oxysporum f. sp. cubense JT1 (FocJT1) is reported. Incubation of FocJT1 mycelium with auric chloride solution produces gold nanoparticles in 60 min. Gold nanoparticles were characterized by UV-Vis spectroscopy, FTIR, and particle size analysis. The particles synthesized were of 22 nm sized, capped by proteins, and posed antimicrobial activity against Pseudomonas sp.

No MeSH data available.


Related in: MedlinePlus

UV-visible spectra recorded as a function of time of reaction of control with fungal biomass in (a) media and (b) media augmented with 10 mM HAuCl4.
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fig2: UV-visible spectra recorded as a function of time of reaction of control with fungal biomass in (a) media and (b) media augmented with 10 mM HAuCl4.

Mentions: Therefore, synthesis of gold nanoparticles was assayed using spectral scan by UV-visible spectroscopy in the spectral region 340–700 nm. This technique outlined above has proved to be very useful for the analysis of nanoparticles [21]. As illustrated in Figure 2(a), the UV-Visible spectra recorded as a function of time of reaction of control having fungal biomass in medium (control) whereas Figure 2(b) shows the UV-Vis spectra of an aqueous solution of 10 mM HAuCl4 with the fungal biomass and medium. A broad peak located between 500 and 580 nm was found to increase with time representing the gold nanoparticles synthesis. The peak was developed in 60 min of reaction sample indicating that the synthesis of gold nanoparticles starts rapidly using FocJT1. The peak reached to near saturation at 90 min reaction time. In earlier studies on the synthesis of silver and gold nanoparticles using bacteria [11] and fungi [12], the time required for completion of the reaction (i.e., complete reduction of the metal ions) ranges from 24 to 120 h.


Biosynthesis of Gold Nanoparticles Using Fusarium oxysporum f. sp. cubense JT1, a Plant Pathogenic Fungus.

Thakker JN, Dalwadi P, Dhandhukia PC - ISRN Biotechnol (2012)

UV-visible spectra recorded as a function of time of reaction of control with fungal biomass in (a) media and (b) media augmented with 10 mM HAuCl4.
© Copyright Policy - open-access
Related In: Results  -  Collection

Show All Figures
getmorefigures.php?uid=PMC4403611&req=5

fig2: UV-visible spectra recorded as a function of time of reaction of control with fungal biomass in (a) media and (b) media augmented with 10 mM HAuCl4.
Mentions: Therefore, synthesis of gold nanoparticles was assayed using spectral scan by UV-visible spectroscopy in the spectral region 340–700 nm. This technique outlined above has proved to be very useful for the analysis of nanoparticles [21]. As illustrated in Figure 2(a), the UV-Visible spectra recorded as a function of time of reaction of control having fungal biomass in medium (control) whereas Figure 2(b) shows the UV-Vis spectra of an aqueous solution of 10 mM HAuCl4 with the fungal biomass and medium. A broad peak located between 500 and 580 nm was found to increase with time representing the gold nanoparticles synthesis. The peak was developed in 60 min of reaction sample indicating that the synthesis of gold nanoparticles starts rapidly using FocJT1. The peak reached to near saturation at 90 min reaction time. In earlier studies on the synthesis of silver and gold nanoparticles using bacteria [11] and fungi [12], the time required for completion of the reaction (i.e., complete reduction of the metal ions) ranges from 24 to 120 h.

Bottom Line: Recently, reports are published on the extracellular as well as intracellular biosynthesis of gold nanoparticles using microorganisms.Incubation of FocJT1 mycelium with auric chloride solution produces gold nanoparticles in 60 min.Gold nanoparticles were characterized by UV-Vis spectroscopy, FTIR, and particle size analysis.

View Article: PubMed Central - PubMed

Affiliation: Department of Biotechnology, P. D. Patel Institute of Applied Science, Charotar University of Science & Technology, Education Campus Changa, Changa 388421, India.

ABSTRACT
The development of reliable processes for the synthesis of gold nanoparticles is an important aspect of current nanotechnology research. Recently, reports are published on the extracellular as well as intracellular biosynthesis of gold nanoparticles using microorganisms. However, these methods of synthesis are rather slow. In present study, rapid and extracellular synthesis of gold nanoparticles using a plant pathogenic fungus F. oxysporum f. sp. cubense JT1 (FocJT1) is reported. Incubation of FocJT1 mycelium with auric chloride solution produces gold nanoparticles in 60 min. Gold nanoparticles were characterized by UV-Vis spectroscopy, FTIR, and particle size analysis. The particles synthesized were of 22 nm sized, capped by proteins, and posed antimicrobial activity against Pseudomonas sp.

No MeSH data available.


Related in: MedlinePlus