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Induction of Defense-Related Enzymes in Banana Plants: Effect of Live and Dead Pathogenic Strain of Fusarium oxysporum f. sp. cubense.

Thakker JN, Patel S, Dhandhukia PC - ISRN Biotechnol (2012)

Bottom Line: Interaction with dead pathogen resulted in escalating defense response in plants.Later on plants inoculated with dead pathogen showed resistance to even forced inoculation of live pathogen.Therefore, preparation from dead pathogen could be a potential candidate as a biocontrol agent or plant vaccine to combat Panama disease.

View Article: PubMed Central - PubMed

Affiliation: Department of Biotechnology, PD Patel Institute of Applied Sciences, Charotar University of Science and Technology, CARUSAT Campus, Gujarat, Changa 388421, India.

ABSTRACT
The aim of the present study was to scrutinize the response of banana (Grand Naine variety) plants when interacting with dead or live pathogen, Fusarium oxysporum f.sp. cubense, a causative agent of Panama disease. Response of plants was evaluated in terms of induction of defense-related marker enzyme activity, namely, peroxidase (POX), polyphenol oxidase (PPO), β-1,3 glucanase, chitinase, and phenolics. Plant's interaction with live pathogen resulted in early induction of defense to restrain penetration as well as antimicrobial productions. However, pathogen overcame the defense of plant and caused disease. Interaction with dead pathogen resulted in escalating defense response in plants. Later on plants inoculated with dead pathogen showed resistance to even forced inoculation of live pathogen. Results obtained in the present study suggest that dead pathogen was able to mount defense response in plants and provide resistance to Panama disease upon subsequent exposure. Therefore, preparation from dead pathogen could be a potential candidate as a biocontrol agent or plant vaccine to combat Panama disease.

No MeSH data available.


Related in: MedlinePlus

PPO activity profile of banana for generation of antimicrobials in response to distilled water (control), live fungus, and dead fungus interactions for seven days.
© Copyright Policy - open-access
Related In: Results  -  Collection


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fig2: PPO activity profile of banana for generation of antimicrobials in response to distilled water (control), live fungus, and dead fungus interactions for seven days.

Mentions: POX activity in control plants remained constant throughout seven days of study. POX activity induced earlier in dead and live pathogen treated plants and retained elevated levels compared to control plants. POX activity increased 4- and 2.5-fold in dead and live fungus treated plants, respectively. Highest POX activity in plant treated with dead and live pathogen was observed on 6th and 7th day, respectively (Figure 1). Comprehensible difference in PPO activity was observed on treatment of dead and live pathogen. With live fungus interaction, plants showed to induce 3-fold PPO activity from first day onward, reached highest level on 4th day, and thereafter reached near basal. However, dead fungus treatment failed to mount significant induction in PPO activity for initial five days of interaction followed by 2- and 3-fold induction in PPO activity on 6th and 7th days, respectively compared to control plants (Figure 2).


Induction of Defense-Related Enzymes in Banana Plants: Effect of Live and Dead Pathogenic Strain of Fusarium oxysporum f. sp. cubense.

Thakker JN, Patel S, Dhandhukia PC - ISRN Biotechnol (2012)

PPO activity profile of banana for generation of antimicrobials in response to distilled water (control), live fungus, and dead fungus interactions for seven days.
© Copyright Policy - open-access
Related In: Results  -  Collection

Show All Figures
getmorefigures.php?uid=PMC4403610&req=5

fig2: PPO activity profile of banana for generation of antimicrobials in response to distilled water (control), live fungus, and dead fungus interactions for seven days.
Mentions: POX activity in control plants remained constant throughout seven days of study. POX activity induced earlier in dead and live pathogen treated plants and retained elevated levels compared to control plants. POX activity increased 4- and 2.5-fold in dead and live fungus treated plants, respectively. Highest POX activity in plant treated with dead and live pathogen was observed on 6th and 7th day, respectively (Figure 1). Comprehensible difference in PPO activity was observed on treatment of dead and live pathogen. With live fungus interaction, plants showed to induce 3-fold PPO activity from first day onward, reached highest level on 4th day, and thereafter reached near basal. However, dead fungus treatment failed to mount significant induction in PPO activity for initial five days of interaction followed by 2- and 3-fold induction in PPO activity on 6th and 7th days, respectively compared to control plants (Figure 2).

Bottom Line: Interaction with dead pathogen resulted in escalating defense response in plants.Later on plants inoculated with dead pathogen showed resistance to even forced inoculation of live pathogen.Therefore, preparation from dead pathogen could be a potential candidate as a biocontrol agent or plant vaccine to combat Panama disease.

View Article: PubMed Central - PubMed

Affiliation: Department of Biotechnology, PD Patel Institute of Applied Sciences, Charotar University of Science and Technology, CARUSAT Campus, Gujarat, Changa 388421, India.

ABSTRACT
The aim of the present study was to scrutinize the response of banana (Grand Naine variety) plants when interacting with dead or live pathogen, Fusarium oxysporum f.sp. cubense, a causative agent of Panama disease. Response of plants was evaluated in terms of induction of defense-related marker enzyme activity, namely, peroxidase (POX), polyphenol oxidase (PPO), β-1,3 glucanase, chitinase, and phenolics. Plant's interaction with live pathogen resulted in early induction of defense to restrain penetration as well as antimicrobial productions. However, pathogen overcame the defense of plant and caused disease. Interaction with dead pathogen resulted in escalating defense response in plants. Later on plants inoculated with dead pathogen showed resistance to even forced inoculation of live pathogen. Results obtained in the present study suggest that dead pathogen was able to mount defense response in plants and provide resistance to Panama disease upon subsequent exposure. Therefore, preparation from dead pathogen could be a potential candidate as a biocontrol agent or plant vaccine to combat Panama disease.

No MeSH data available.


Related in: MedlinePlus