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Comparison of Lipase Production by Enterococcus faecium MTCC 5695 and Pediococcus acidilactici MTCC 11361 Using Fish Waste as Substrate: Optimization of Culture Conditions by Response Surface Methodology.

Ramakrishnan V, Goveas LC, Narayan B, Halami PM - ISRN Biotechnol (2012)

Bottom Line: A medium using fish waste as substrate was designed for production of lipase by Enterococcus faecium MTCC 5695 and Pediococcus acidilactici MTCC 11361.The levels of these factors were optimized further by Box-Behnken design using response surface methodology (RSM).Optimized conditions were found to be 5% v/v FWO, 0.15 mg/mL FWPH and 24 h of fermentation time for MTCC 5695, and 4% v/v FWO, 0.15 mg/mL FWPH and 24 h of fermentation for MTCC 11361, which were further validated.

View Article: PubMed Central - PubMed

Affiliation: Department of Food Microbiology, Central Food Technological Research Institute (CSIR), Mysore 570 020, India.

ABSTRACT
A medium using fish waste as substrate was designed for production of lipase by Enterococcus faecium MTCC 5695 and Pediococcus acidilactici MTCC 11361. Medium components and culture conditions (fish waste protein hydrolysate (FWPH) concentration, fish waste oil (FWO) concentration, pH, temperature, and fermentation time) which affect lipase production were screened using factorial (5 factors ∗ 2 levels) design of which FWPH concentration, FWO concentration, and fermentation time showed significance (P < 0.05). The levels of these factors were optimized further by Box-Behnken design using response surface methodology (RSM). Optimized conditions were found to be 5% v/v FWO, 0.15 mg/mL FWPH and 24 h of fermentation time for MTCC 5695, and 4% v/v FWO, 0.15 mg/mL FWPH and 24 h of fermentation for MTCC 11361, which were further validated. Under optimized conditions, MTCC 5695 and MTCC 11361 showed 3.15- (543.63 to 1715 U/mL) and 2.3- (214.74 to 493 U/mL) fold increase in lipase production, respectively, as compared to unoptimized conditions.

No MeSH data available.


Related in: MedlinePlus

Three-dimensional plot showing the effect of: (a) FWPH concentration, FWO concentration; (b) FWO concentration, time; on lipase production by MTCC 5695 (c) FWPH concentration, FWO concentration; (d) FWO concentration, time; on lipase production by MTCC 11361. (FWPH: fish waste protein hydrolysates; FWO: fish waste oil).
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fig1: Three-dimensional plot showing the effect of: (a) FWPH concentration, FWO concentration; (b) FWO concentration, time; on lipase production by MTCC 5695 (c) FWPH concentration, FWO concentration; (d) FWO concentration, time; on lipase production by MTCC 11361. (FWPH: fish waste protein hydrolysates; FWO: fish waste oil).

Mentions: The response surface graph for Y1 and Y2 as a function of FWPH concentration and FWO concentration is presented in Figures 1(a) and 1(c), respectively. It was observed that lipase production increased with increase in FWPH concentration up to 0.16 mg/mL beyond which there was a decrease in case of both the organisms probably due to inhibition of enzyme activity in the presence of excess nitrogen. A possible mechanism may be that FWPH is a complex nitrogen source and the cells may produce more protease for the degradation of FWPH before its utilization. This might result in lower production and higher degradation of the lipase [30]. The lipase production increased with increase in FWO concentration for MTCC 5695, whereas lipase production increased with increase in FWO concentration up to 4% v/v beyond which there was a decrease for MTCC 11361. The decrease in lipase production by MTCC 11361 beyond 4% v/v of FWO concentration may be due to the reason that high concentrations of FWO have more incidence of long chain fatty acids which might have an inhibitory effect on lipase production [5]. However, MTCC 5695 was found to be more tolerant to FWO. The influence of time and FWO concentration on Y1 and Y2 is presented in Figures 1(b) and 1(d), respectively. The figure clearly indicates that lipase production decreases with increase in time for MTCC 5695 however, a slight increase was observed after 48 h for MTCC 11361. This may be probably due to the fact that MTCC 5695 and MTCC 11361 achieve maximum growth in 24 h after which they enter the stationary phase resulting in a steady decline in lipase production.


Comparison of Lipase Production by Enterococcus faecium MTCC 5695 and Pediococcus acidilactici MTCC 11361 Using Fish Waste as Substrate: Optimization of Culture Conditions by Response Surface Methodology.

Ramakrishnan V, Goveas LC, Narayan B, Halami PM - ISRN Biotechnol (2012)

Three-dimensional plot showing the effect of: (a) FWPH concentration, FWO concentration; (b) FWO concentration, time; on lipase production by MTCC 5695 (c) FWPH concentration, FWO concentration; (d) FWO concentration, time; on lipase production by MTCC 11361. (FWPH: fish waste protein hydrolysates; FWO: fish waste oil).
© Copyright Policy - open-access
Related In: Results  -  Collection

Show All Figures
getmorefigures.php?uid=PMC4403595&req=5

fig1: Three-dimensional plot showing the effect of: (a) FWPH concentration, FWO concentration; (b) FWO concentration, time; on lipase production by MTCC 5695 (c) FWPH concentration, FWO concentration; (d) FWO concentration, time; on lipase production by MTCC 11361. (FWPH: fish waste protein hydrolysates; FWO: fish waste oil).
Mentions: The response surface graph for Y1 and Y2 as a function of FWPH concentration and FWO concentration is presented in Figures 1(a) and 1(c), respectively. It was observed that lipase production increased with increase in FWPH concentration up to 0.16 mg/mL beyond which there was a decrease in case of both the organisms probably due to inhibition of enzyme activity in the presence of excess nitrogen. A possible mechanism may be that FWPH is a complex nitrogen source and the cells may produce more protease for the degradation of FWPH before its utilization. This might result in lower production and higher degradation of the lipase [30]. The lipase production increased with increase in FWO concentration for MTCC 5695, whereas lipase production increased with increase in FWO concentration up to 4% v/v beyond which there was a decrease for MTCC 11361. The decrease in lipase production by MTCC 11361 beyond 4% v/v of FWO concentration may be due to the reason that high concentrations of FWO have more incidence of long chain fatty acids which might have an inhibitory effect on lipase production [5]. However, MTCC 5695 was found to be more tolerant to FWO. The influence of time and FWO concentration on Y1 and Y2 is presented in Figures 1(b) and 1(d), respectively. The figure clearly indicates that lipase production decreases with increase in time for MTCC 5695 however, a slight increase was observed after 48 h for MTCC 11361. This may be probably due to the fact that MTCC 5695 and MTCC 11361 achieve maximum growth in 24 h after which they enter the stationary phase resulting in a steady decline in lipase production.

Bottom Line: A medium using fish waste as substrate was designed for production of lipase by Enterococcus faecium MTCC 5695 and Pediococcus acidilactici MTCC 11361.The levels of these factors were optimized further by Box-Behnken design using response surface methodology (RSM).Optimized conditions were found to be 5% v/v FWO, 0.15 mg/mL FWPH and 24 h of fermentation time for MTCC 5695, and 4% v/v FWO, 0.15 mg/mL FWPH and 24 h of fermentation for MTCC 11361, which were further validated.

View Article: PubMed Central - PubMed

Affiliation: Department of Food Microbiology, Central Food Technological Research Institute (CSIR), Mysore 570 020, India.

ABSTRACT
A medium using fish waste as substrate was designed for production of lipase by Enterococcus faecium MTCC 5695 and Pediococcus acidilactici MTCC 11361. Medium components and culture conditions (fish waste protein hydrolysate (FWPH) concentration, fish waste oil (FWO) concentration, pH, temperature, and fermentation time) which affect lipase production were screened using factorial (5 factors ∗ 2 levels) design of which FWPH concentration, FWO concentration, and fermentation time showed significance (P < 0.05). The levels of these factors were optimized further by Box-Behnken design using response surface methodology (RSM). Optimized conditions were found to be 5% v/v FWO, 0.15 mg/mL FWPH and 24 h of fermentation time for MTCC 5695, and 4% v/v FWO, 0.15 mg/mL FWPH and 24 h of fermentation for MTCC 11361, which were further validated. Under optimized conditions, MTCC 5695 and MTCC 11361 showed 3.15- (543.63 to 1715 U/mL) and 2.3- (214.74 to 493 U/mL) fold increase in lipase production, respectively, as compared to unoptimized conditions.

No MeSH data available.


Related in: MedlinePlus