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Effects of Photoperiod on Epididymal and Sperm Morphology in a Wild Rodent, the Viscacha (Lagostomus maximus maximus).

Cruceño AM, de Rosas JC, Fóscolo M, Chaves EM, Scardapane L, Dominguez S, Aguilera-Merlo C - ISRN Anat (2012)

Bottom Line: Under light and electron microscopy, epididymal corpus and cauda showed marked seasonal variations in structural parameters and in the distribution of different cellular populations of epithelium.Initial and caput segments showed mild morphological variations between the two periods.During this period, anomalies were found mainly in the head, midpiece, and neck, while in the activity period, defects were found only in the head.

View Article: PubMed Central - PubMed

Affiliation: Cátedra de Histología, Facultad de Química, Bioquímica y Farmacia, Universidad Nacional de San Luis, Avenida Ejército de Los Andes 950-No. 2 Piso, 5700 San Luis, Argentina.

ABSTRACT
The viscacha (Lagostomus maximus maximus) is a seasonal South American wild rodent. The adult males exhibit an annual reproductive cycle with periods of maximum and minimum gonadal activity. Four segments have been identified in the epididymis of this species: initial, caput, corpus, and cauda. The main objective of this work was to relate the seasonal morphological changes observed in the epididymal duct with the data from epididymal sperm during periods of activity and gonadal regression using light and scanning electron microscopy (SEM). Under light and electron microscopy, epididymal corpus and cauda showed marked seasonal variations in structural parameters and in the distribution of different cellular populations of epithelium. Initial and caput segments showed mild morphological variations between the two periods. Changes in epididymal sperm morphology were observed in the periods analyzed and an increased number of abnormal gametes were found during the regression period. During this period, anomalies were found mainly in the head, midpiece, and neck, while in the activity period, defects were found only in the head. Our results confirm that the morphological characteristics of the epididymal segments, as well as sperm morphology, undergo significant changes during the reproductive cycle of Lagostomus.

No MeSH data available.


Related in: MedlinePlus

Scanning electron micrograph of the epididymal cauda during the period of regression. (a) Low magnification image showing epididymal sections (asterisk) with reduced luminal diameter in relation to the observed during the active period. L: lumen. SEM: ×300. (b) In the epididymal lumen at higher magnification is observed low sperm content (sp) and abundant germ cells (g) detached. SEM: ×6000. (c) Semithin cut obtained in the same region of the epididymal cauda showing principal cells (P) and numerous clear cells (Cl) with lax and irregular nuclei (n). Note the low content of sperm (sp) in the lumen (L) and increased thickness of the lamina propria (Lp), in relation to the observed during the active period. s stereocilia. TB: ×1000.
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fig7: Scanning electron micrograph of the epididymal cauda during the period of regression. (a) Low magnification image showing epididymal sections (asterisk) with reduced luminal diameter in relation to the observed during the active period. L: lumen. SEM: ×300. (b) In the epididymal lumen at higher magnification is observed low sperm content (sp) and abundant germ cells (g) detached. SEM: ×6000. (c) Semithin cut obtained in the same region of the epididymal cauda showing principal cells (P) and numerous clear cells (Cl) with lax and irregular nuclei (n). Note the low content of sperm (sp) in the lumen (L) and increased thickness of the lamina propria (Lp), in relation to the observed during the active period. s stereocilia. TB: ×1000.

Mentions: During the regression period, the epididymal cauda exhibits important morphological changes as compared to the active period. SEM images revealed a significant reduction of the luminal diameter (Figure 7(a)). The lumen exhibits lower presence of stored sperm cells and abundant detached epithelial cells of testicular and epididymal origin (Figure 7(b)). In this period, a higher presence of clear cells was observed in the cauda epididymal epithelium as compared to the active period (Figure 7(c)).


Effects of Photoperiod on Epididymal and Sperm Morphology in a Wild Rodent, the Viscacha (Lagostomus maximus maximus).

Cruceño AM, de Rosas JC, Fóscolo M, Chaves EM, Scardapane L, Dominguez S, Aguilera-Merlo C - ISRN Anat (2012)

Scanning electron micrograph of the epididymal cauda during the period of regression. (a) Low magnification image showing epididymal sections (asterisk) with reduced luminal diameter in relation to the observed during the active period. L: lumen. SEM: ×300. (b) In the epididymal lumen at higher magnification is observed low sperm content (sp) and abundant germ cells (g) detached. SEM: ×6000. (c) Semithin cut obtained in the same region of the epididymal cauda showing principal cells (P) and numerous clear cells (Cl) with lax and irregular nuclei (n). Note the low content of sperm (sp) in the lumen (L) and increased thickness of the lamina propria (Lp), in relation to the observed during the active period. s stereocilia. TB: ×1000.
© Copyright Policy - open-access
Related In: Results  -  Collection

Show All Figures
getmorefigures.php?uid=PMC4403560&req=5

fig7: Scanning electron micrograph of the epididymal cauda during the period of regression. (a) Low magnification image showing epididymal sections (asterisk) with reduced luminal diameter in relation to the observed during the active period. L: lumen. SEM: ×300. (b) In the epididymal lumen at higher magnification is observed low sperm content (sp) and abundant germ cells (g) detached. SEM: ×6000. (c) Semithin cut obtained in the same region of the epididymal cauda showing principal cells (P) and numerous clear cells (Cl) with lax and irregular nuclei (n). Note the low content of sperm (sp) in the lumen (L) and increased thickness of the lamina propria (Lp), in relation to the observed during the active period. s stereocilia. TB: ×1000.
Mentions: During the regression period, the epididymal cauda exhibits important morphological changes as compared to the active period. SEM images revealed a significant reduction of the luminal diameter (Figure 7(a)). The lumen exhibits lower presence of stored sperm cells and abundant detached epithelial cells of testicular and epididymal origin (Figure 7(b)). In this period, a higher presence of clear cells was observed in the cauda epididymal epithelium as compared to the active period (Figure 7(c)).

Bottom Line: Under light and electron microscopy, epididymal corpus and cauda showed marked seasonal variations in structural parameters and in the distribution of different cellular populations of epithelium.Initial and caput segments showed mild morphological variations between the two periods.During this period, anomalies were found mainly in the head, midpiece, and neck, while in the activity period, defects were found only in the head.

View Article: PubMed Central - PubMed

Affiliation: Cátedra de Histología, Facultad de Química, Bioquímica y Farmacia, Universidad Nacional de San Luis, Avenida Ejército de Los Andes 950-No. 2 Piso, 5700 San Luis, Argentina.

ABSTRACT
The viscacha (Lagostomus maximus maximus) is a seasonal South American wild rodent. The adult males exhibit an annual reproductive cycle with periods of maximum and minimum gonadal activity. Four segments have been identified in the epididymis of this species: initial, caput, corpus, and cauda. The main objective of this work was to relate the seasonal morphological changes observed in the epididymal duct with the data from epididymal sperm during periods of activity and gonadal regression using light and scanning electron microscopy (SEM). Under light and electron microscopy, epididymal corpus and cauda showed marked seasonal variations in structural parameters and in the distribution of different cellular populations of epithelium. Initial and caput segments showed mild morphological variations between the two periods. Changes in epididymal sperm morphology were observed in the periods analyzed and an increased number of abnormal gametes were found during the regression period. During this period, anomalies were found mainly in the head, midpiece, and neck, while in the activity period, defects were found only in the head. Our results confirm that the morphological characteristics of the epididymal segments, as well as sperm morphology, undergo significant changes during the reproductive cycle of Lagostomus.

No MeSH data available.


Related in: MedlinePlus