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Effects of Photoperiod on Epididymal and Sperm Morphology in a Wild Rodent, the Viscacha (Lagostomus maximus maximus).

Cruceño AM, de Rosas JC, Fóscolo M, Chaves EM, Scardapane L, Dominguez S, Aguilera-Merlo C - ISRN Anat (2012)

Bottom Line: Under light and electron microscopy, epididymal corpus and cauda showed marked seasonal variations in structural parameters and in the distribution of different cellular populations of epithelium.Initial and caput segments showed mild morphological variations between the two periods.During this period, anomalies were found mainly in the head, midpiece, and neck, while in the activity period, defects were found only in the head.

View Article: PubMed Central - PubMed

Affiliation: Cátedra de Histología, Facultad de Química, Bioquímica y Farmacia, Universidad Nacional de San Luis, Avenida Ejército de Los Andes 950-No. 2 Piso, 5700 San Luis, Argentina.

ABSTRACT
The viscacha (Lagostomus maximus maximus) is a seasonal South American wild rodent. The adult males exhibit an annual reproductive cycle with periods of maximum and minimum gonadal activity. Four segments have been identified in the epididymis of this species: initial, caput, corpus, and cauda. The main objective of this work was to relate the seasonal morphological changes observed in the epididymal duct with the data from epididymal sperm during periods of activity and gonadal regression using light and scanning electron microscopy (SEM). Under light and electron microscopy, epididymal corpus and cauda showed marked seasonal variations in structural parameters and in the distribution of different cellular populations of epithelium. Initial and caput segments showed mild morphological variations between the two periods. Changes in epididymal sperm morphology were observed in the periods analyzed and an increased number of abnormal gametes were found during the regression period. During this period, anomalies were found mainly in the head, midpiece, and neck, while in the activity period, defects were found only in the head. Our results confirm that the morphological characteristics of the epididymal segments, as well as sperm morphology, undergo significant changes during the reproductive cycle of Lagostomus.

No MeSH data available.


Related in: MedlinePlus

Epididymal corpus in gonadal regression period. (a) Semithin cut showing irregular nuclei (n) and abundant cytoplasmic vacuoles (v) in principal cells (P). Note the greater height of the epithelium in relation to that observed in the active period. L: lumen; s: stereocilia; Lp: lamina propria. TB: ×1000. (b) Scanning electron micrograph showing holes or depressions (asterisks) in the cytoplasmic surface of epithelial cells caused by marked epithelial vacuolation. L: lumen. SEM: ×3500.
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fig5: Epididymal corpus in gonadal regression period. (a) Semithin cut showing irregular nuclei (n) and abundant cytoplasmic vacuoles (v) in principal cells (P). Note the greater height of the epithelium in relation to that observed in the active period. L: lumen; s: stereocilia; Lp: lamina propria. TB: ×1000. (b) Scanning electron micrograph showing holes or depressions (asterisks) in the cytoplasmic surface of epithelial cells caused by marked epithelial vacuolation. L: lumen. SEM: ×3500.

Mentions: In the regression period, the principal cells exhibited nuclei with irregular edges, and the cytoplasm shows abundant vacuoles of irregular sizes and shapes, sometimes fused with each other (Figure 5(a)). The lumen and lamina propria show characteristics similar to those described for the active period. Under the scanning electronic microscope, a wider extension of principal cells with lengthened cellular apex are observed. The cytoplasmic surface exhibits depressions or holes that can be related with the large vacuoles present in their cytoplasm (Figure 5(b)).


Effects of Photoperiod on Epididymal and Sperm Morphology in a Wild Rodent, the Viscacha (Lagostomus maximus maximus).

Cruceño AM, de Rosas JC, Fóscolo M, Chaves EM, Scardapane L, Dominguez S, Aguilera-Merlo C - ISRN Anat (2012)

Epididymal corpus in gonadal regression period. (a) Semithin cut showing irregular nuclei (n) and abundant cytoplasmic vacuoles (v) in principal cells (P). Note the greater height of the epithelium in relation to that observed in the active period. L: lumen; s: stereocilia; Lp: lamina propria. TB: ×1000. (b) Scanning electron micrograph showing holes or depressions (asterisks) in the cytoplasmic surface of epithelial cells caused by marked epithelial vacuolation. L: lumen. SEM: ×3500.
© Copyright Policy - open-access
Related In: Results  -  Collection

Show All Figures
getmorefigures.php?uid=PMC4403560&req=5

fig5: Epididymal corpus in gonadal regression period. (a) Semithin cut showing irregular nuclei (n) and abundant cytoplasmic vacuoles (v) in principal cells (P). Note the greater height of the epithelium in relation to that observed in the active period. L: lumen; s: stereocilia; Lp: lamina propria. TB: ×1000. (b) Scanning electron micrograph showing holes or depressions (asterisks) in the cytoplasmic surface of epithelial cells caused by marked epithelial vacuolation. L: lumen. SEM: ×3500.
Mentions: In the regression period, the principal cells exhibited nuclei with irregular edges, and the cytoplasm shows abundant vacuoles of irregular sizes and shapes, sometimes fused with each other (Figure 5(a)). The lumen and lamina propria show characteristics similar to those described for the active period. Under the scanning electronic microscope, a wider extension of principal cells with lengthened cellular apex are observed. The cytoplasmic surface exhibits depressions or holes that can be related with the large vacuoles present in their cytoplasm (Figure 5(b)).

Bottom Line: Under light and electron microscopy, epididymal corpus and cauda showed marked seasonal variations in structural parameters and in the distribution of different cellular populations of epithelium.Initial and caput segments showed mild morphological variations between the two periods.During this period, anomalies were found mainly in the head, midpiece, and neck, while in the activity period, defects were found only in the head.

View Article: PubMed Central - PubMed

Affiliation: Cátedra de Histología, Facultad de Química, Bioquímica y Farmacia, Universidad Nacional de San Luis, Avenida Ejército de Los Andes 950-No. 2 Piso, 5700 San Luis, Argentina.

ABSTRACT
The viscacha (Lagostomus maximus maximus) is a seasonal South American wild rodent. The adult males exhibit an annual reproductive cycle with periods of maximum and minimum gonadal activity. Four segments have been identified in the epididymis of this species: initial, caput, corpus, and cauda. The main objective of this work was to relate the seasonal morphological changes observed in the epididymal duct with the data from epididymal sperm during periods of activity and gonadal regression using light and scanning electron microscopy (SEM). Under light and electron microscopy, epididymal corpus and cauda showed marked seasonal variations in structural parameters and in the distribution of different cellular populations of epithelium. Initial and caput segments showed mild morphological variations between the two periods. Changes in epididymal sperm morphology were observed in the periods analyzed and an increased number of abnormal gametes were found during the regression period. During this period, anomalies were found mainly in the head, midpiece, and neck, while in the activity period, defects were found only in the head. Our results confirm that the morphological characteristics of the epididymal segments, as well as sperm morphology, undergo significant changes during the reproductive cycle of Lagostomus.

No MeSH data available.


Related in: MedlinePlus