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Effects of Photoperiod on Epididymal and Sperm Morphology in a Wild Rodent, the Viscacha (Lagostomus maximus maximus).

Cruceño AM, de Rosas JC, Fóscolo M, Chaves EM, Scardapane L, Dominguez S, Aguilera-Merlo C - ISRN Anat (2012)

Bottom Line: Under light and electron microscopy, epididymal corpus and cauda showed marked seasonal variations in structural parameters and in the distribution of different cellular populations of epithelium.Initial and caput segments showed mild morphological variations between the two periods.During this period, anomalies were found mainly in the head, midpiece, and neck, while in the activity period, defects were found only in the head.

View Article: PubMed Central - PubMed

Affiliation: Cátedra de Histología, Facultad de Química, Bioquímica y Farmacia, Universidad Nacional de San Luis, Avenida Ejército de Los Andes 950-No. 2 Piso, 5700 San Luis, Argentina.

ABSTRACT
The viscacha (Lagostomus maximus maximus) is a seasonal South American wild rodent. The adult males exhibit an annual reproductive cycle with periods of maximum and minimum gonadal activity. Four segments have been identified in the epididymis of this species: initial, caput, corpus, and cauda. The main objective of this work was to relate the seasonal morphological changes observed in the epididymal duct with the data from epididymal sperm during periods of activity and gonadal regression using light and scanning electron microscopy (SEM). Under light and electron microscopy, epididymal corpus and cauda showed marked seasonal variations in structural parameters and in the distribution of different cellular populations of epithelium. Initial and caput segments showed mild morphological variations between the two periods. Changes in epididymal sperm morphology were observed in the periods analyzed and an increased number of abnormal gametes were found during the regression period. During this period, anomalies were found mainly in the head, midpiece, and neck, while in the activity period, defects were found only in the head. Our results confirm that the morphological characteristics of the epididymal segments, as well as sperm morphology, undergo significant changes during the reproductive cycle of Lagostomus.

No MeSH data available.


Related in: MedlinePlus

Principal cell (P) in the epididymal epithelium. Image observed to scanning electron micrograph showing the morphology of this cell. Note the presence of extensive stereocilia (s) on the apical surface of this cell. SEM: ×7000.
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fig2: Principal cell (P) in the epididymal epithelium. Image observed to scanning electron micrograph showing the morphology of this cell. Note the presence of extensive stereocilia (s) on the apical surface of this cell. SEM: ×7000.

Mentions: During the period of gonadal activity, these segments showed high epithelium, with principal cells as the predominant type (Figures 1(a) and 1(b)). These cells have an elongated nucleus perpendicular to the lamina propria and in the apical surface are located stereocilia long and thin (Figure 2). In semithin sections it can be observed that the principal and halo cells show great development of their cellular components, which indicates intense cellular activity during this period. Numerous structures similar to lipid cells and dense bodies were observed in the cytoplasm of the principal cells. An important number of spermatozoa are in contact with long stereocilia of these cells (Figures 1(b)–3(a)).


Effects of Photoperiod on Epididymal and Sperm Morphology in a Wild Rodent, the Viscacha (Lagostomus maximus maximus).

Cruceño AM, de Rosas JC, Fóscolo M, Chaves EM, Scardapane L, Dominguez S, Aguilera-Merlo C - ISRN Anat (2012)

Principal cell (P) in the epididymal epithelium. Image observed to scanning electron micrograph showing the morphology of this cell. Note the presence of extensive stereocilia (s) on the apical surface of this cell. SEM: ×7000.
© Copyright Policy - open-access
Related In: Results  -  Collection

Show All Figures
getmorefigures.php?uid=PMC4403560&req=5

fig2: Principal cell (P) in the epididymal epithelium. Image observed to scanning electron micrograph showing the morphology of this cell. Note the presence of extensive stereocilia (s) on the apical surface of this cell. SEM: ×7000.
Mentions: During the period of gonadal activity, these segments showed high epithelium, with principal cells as the predominant type (Figures 1(a) and 1(b)). These cells have an elongated nucleus perpendicular to the lamina propria and in the apical surface are located stereocilia long and thin (Figure 2). In semithin sections it can be observed that the principal and halo cells show great development of their cellular components, which indicates intense cellular activity during this period. Numerous structures similar to lipid cells and dense bodies were observed in the cytoplasm of the principal cells. An important number of spermatozoa are in contact with long stereocilia of these cells (Figures 1(b)–3(a)).

Bottom Line: Under light and electron microscopy, epididymal corpus and cauda showed marked seasonal variations in structural parameters and in the distribution of different cellular populations of epithelium.Initial and caput segments showed mild morphological variations between the two periods.During this period, anomalies were found mainly in the head, midpiece, and neck, while in the activity period, defects were found only in the head.

View Article: PubMed Central - PubMed

Affiliation: Cátedra de Histología, Facultad de Química, Bioquímica y Farmacia, Universidad Nacional de San Luis, Avenida Ejército de Los Andes 950-No. 2 Piso, 5700 San Luis, Argentina.

ABSTRACT
The viscacha (Lagostomus maximus maximus) is a seasonal South American wild rodent. The adult males exhibit an annual reproductive cycle with periods of maximum and minimum gonadal activity. Four segments have been identified in the epididymis of this species: initial, caput, corpus, and cauda. The main objective of this work was to relate the seasonal morphological changes observed in the epididymal duct with the data from epididymal sperm during periods of activity and gonadal regression using light and scanning electron microscopy (SEM). Under light and electron microscopy, epididymal corpus and cauda showed marked seasonal variations in structural parameters and in the distribution of different cellular populations of epithelium. Initial and caput segments showed mild morphological variations between the two periods. Changes in epididymal sperm morphology were observed in the periods analyzed and an increased number of abnormal gametes were found during the regression period. During this period, anomalies were found mainly in the head, midpiece, and neck, while in the activity period, defects were found only in the head. Our results confirm that the morphological characteristics of the epididymal segments, as well as sperm morphology, undergo significant changes during the reproductive cycle of Lagostomus.

No MeSH data available.


Related in: MedlinePlus