Limits...
Molecular characterization of eimeria species naturally infecting egyptian baldi chickens.

Gadelhaq SM, Arafa WM, Aboelhadid SM - Iran J Parasitol (2015 Jan-Mar)

Bottom Line: Only single nucleotide substitution was observed among the Egyptian E. tenella isolates (A181G) when compared to the reference one.The Egyptian isolates acquired 4 unique mutations (A68T, C164T, G190A and C227G) in compared with the reference sequence.This is the first time to identify the 7 species of Eimeria from Egyptian baladi chickens.

View Article: PubMed Central - PubMed

Affiliation: Parasitology Department, Faculty of Veterinary Medicine, Beni-Suef University, Egypt.

ABSTRACT

Background: Coccidiosis is a serious protozoal disease of poultry. The identification of Eimeria species has important implications for diagnosis and control as well as for epidemiology. The molecular characterization of Eimeria species infecting Egyptian baladi chickens was investigated.

Methods: Eimeria species oocysts were harvested from intestines of naturally infected Egyptian baldi chickens. The morphometry characterization of oocysts along with COCCIMORPH software was done. The DNA was extracted initially by freezing and thawing then the prepared samples was subjected to commercial DNA kits. The DNA products were analyzed through conventional polymerase chain reaction by using amplified region (SCAR) marker.

Results: The PCR results confirmed the presence of 7 Eimeria species in the examined fecal samples of Egyptian baldi breed with their specific ampilicon sizes being E. acervulina (811bp), E. brunette (626bp), E. tenella (539bp), E. maxima (272bp), E. necatrix (200bp), E. mitis (327bp) and E. praecopx (354bp). A sequencing of the two most predominant species of Eimeria was done, on E. tenella and E. máxima. Analysis of the obtained sequences revealed high identities 99% between Egyptian isolates and the reference one. Similarly, E. maxima isolated from Egyptian baldi chickens showed 98% nucleotide identities with the reference strain. Only single nucleotide substitution was observed among the Egyptian E. tenella isolates (A181G) when compared to the reference one. The Egyptian isolates acquired 4 unique mutations (A68T, C164T, G190A and C227G) in compared with the reference sequence.

Conclusion: This is the first time to identify the 7 species of Eimeria from Egyptian baladi chickens.

No MeSH data available.


Related in: MedlinePlus

A. Amplicone of E. praecox (354bp). Lane M molecular weight marker 100bp, lane C control positive for E. praecox. Lane 4 represents the pool No 4, the only positive pool for E. praecox from 14 pool. B. Amplicone of E. mitis (327bp). Lane M molecular weight marker 100bp, lane C control positive for E. mitis. Lane 1a represent pool No 1, lane 6 b represent pool No 13 C. Amplicone of E. maxima (272bp). Lane M molecular weight marker 100bp, lane C control positive for E. maxima. Pools No 1, 2, 3, 5, 6, 7, 10 and 11 were positive for E. maxima. D. Amplicone of E. necatrix (200 bp). Lane M molecular weight marker 100bp, lane C control positive for E. necatrix. Pools No 1, 3, 4, 5, 8, 9 and 10 were positive for E. necatrix
© Copyright Policy
Related In: Results  -  Collection


getmorefigures.php?uid=PMC4403544&req=5

Figure 2: A. Amplicone of E. praecox (354bp). Lane M molecular weight marker 100bp, lane C control positive for E. praecox. Lane 4 represents the pool No 4, the only positive pool for E. praecox from 14 pool. B. Amplicone of E. mitis (327bp). Lane M molecular weight marker 100bp, lane C control positive for E. mitis. Lane 1a represent pool No 1, lane 6 b represent pool No 13 C. Amplicone of E. maxima (272bp). Lane M molecular weight marker 100bp, lane C control positive for E. maxima. Pools No 1, 2, 3, 5, 6, 7, 10 and 11 were positive for E. maxima. D. Amplicone of E. necatrix (200 bp). Lane M molecular weight marker 100bp, lane C control positive for E. necatrix. Pools No 1, 3, 4, 5, 8, 9 and 10 were positive for E. necatrix

Mentions: The results of molecular identification proved the presence of 7 Eimeria species in the examined Egyptian baldi chicken fecal samples. The primers were sufficiently sensitive and specific enabling the discrimination of seven Eimeria species. The amplified fragments presented different sizes: E. acervulina (811 bp), E. brunette (626 bp), E. tenella (539 bp), E. mitis (310 bp), E. praecox (354 bp), E. maxima (272 bp) and E. necatrix (200 bp) (Fig. 1 & Fig. 2).


Molecular characterization of eimeria species naturally infecting egyptian baldi chickens.

Gadelhaq SM, Arafa WM, Aboelhadid SM - Iran J Parasitol (2015 Jan-Mar)

A. Amplicone of E. praecox (354bp). Lane M molecular weight marker 100bp, lane C control positive for E. praecox. Lane 4 represents the pool No 4, the only positive pool for E. praecox from 14 pool. B. Amplicone of E. mitis (327bp). Lane M molecular weight marker 100bp, lane C control positive for E. mitis. Lane 1a represent pool No 1, lane 6 b represent pool No 13 C. Amplicone of E. maxima (272bp). Lane M molecular weight marker 100bp, lane C control positive for E. maxima. Pools No 1, 2, 3, 5, 6, 7, 10 and 11 were positive for E. maxima. D. Amplicone of E. necatrix (200 bp). Lane M molecular weight marker 100bp, lane C control positive for E. necatrix. Pools No 1, 3, 4, 5, 8, 9 and 10 were positive for E. necatrix
© Copyright Policy
Related In: Results  -  Collection

Show All Figures
getmorefigures.php?uid=PMC4403544&req=5

Figure 2: A. Amplicone of E. praecox (354bp). Lane M molecular weight marker 100bp, lane C control positive for E. praecox. Lane 4 represents the pool No 4, the only positive pool for E. praecox from 14 pool. B. Amplicone of E. mitis (327bp). Lane M molecular weight marker 100bp, lane C control positive for E. mitis. Lane 1a represent pool No 1, lane 6 b represent pool No 13 C. Amplicone of E. maxima (272bp). Lane M molecular weight marker 100bp, lane C control positive for E. maxima. Pools No 1, 2, 3, 5, 6, 7, 10 and 11 were positive for E. maxima. D. Amplicone of E. necatrix (200 bp). Lane M molecular weight marker 100bp, lane C control positive for E. necatrix. Pools No 1, 3, 4, 5, 8, 9 and 10 were positive for E. necatrix
Mentions: The results of molecular identification proved the presence of 7 Eimeria species in the examined Egyptian baldi chicken fecal samples. The primers were sufficiently sensitive and specific enabling the discrimination of seven Eimeria species. The amplified fragments presented different sizes: E. acervulina (811 bp), E. brunette (626 bp), E. tenella (539 bp), E. mitis (310 bp), E. praecox (354 bp), E. maxima (272 bp) and E. necatrix (200 bp) (Fig. 1 & Fig. 2).

Bottom Line: Only single nucleotide substitution was observed among the Egyptian E. tenella isolates (A181G) when compared to the reference one.The Egyptian isolates acquired 4 unique mutations (A68T, C164T, G190A and C227G) in compared with the reference sequence.This is the first time to identify the 7 species of Eimeria from Egyptian baladi chickens.

View Article: PubMed Central - PubMed

Affiliation: Parasitology Department, Faculty of Veterinary Medicine, Beni-Suef University, Egypt.

ABSTRACT

Background: Coccidiosis is a serious protozoal disease of poultry. The identification of Eimeria species has important implications for diagnosis and control as well as for epidemiology. The molecular characterization of Eimeria species infecting Egyptian baladi chickens was investigated.

Methods: Eimeria species oocysts were harvested from intestines of naturally infected Egyptian baldi chickens. The morphometry characterization of oocysts along with COCCIMORPH software was done. The DNA was extracted initially by freezing and thawing then the prepared samples was subjected to commercial DNA kits. The DNA products were analyzed through conventional polymerase chain reaction by using amplified region (SCAR) marker.

Results: The PCR results confirmed the presence of 7 Eimeria species in the examined fecal samples of Egyptian baldi breed with their specific ampilicon sizes being E. acervulina (811bp), E. brunette (626bp), E. tenella (539bp), E. maxima (272bp), E. necatrix (200bp), E. mitis (327bp) and E. praecopx (354bp). A sequencing of the two most predominant species of Eimeria was done, on E. tenella and E. máxima. Analysis of the obtained sequences revealed high identities 99% between Egyptian isolates and the reference one. Similarly, E. maxima isolated from Egyptian baldi chickens showed 98% nucleotide identities with the reference strain. Only single nucleotide substitution was observed among the Egyptian E. tenella isolates (A181G) when compared to the reference one. The Egyptian isolates acquired 4 unique mutations (A68T, C164T, G190A and C227G) in compared with the reference sequence.

Conclusion: This is the first time to identify the 7 species of Eimeria from Egyptian baladi chickens.

No MeSH data available.


Related in: MedlinePlus