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Genetic Identification of Orientobilharzia turkestanicum from Sheep Isolates in Iran.

Tabaripour R, Youssefi MR, Tabaripour R - Iran J Parasitol (2015 Jan-Mar)

Bottom Line: Orientobilharziasis causes significant economic losses to livestock industry of Iran.The mitochondrial cox1 and nad1 DNA were amplified by polymerase chain reaction (PCR) and then sequenced and compared with O. turkestanicum and that of other members of the Schistosomatidae available in Gen-Bank(™).Comparison of nad1 and cox1 sequences of O. turkestanicum obtained in this study with corresponding sequences available in Genbank(™) revealed some sequence variations and provided evidence for presence of microvarients in Iran.

View Article: PubMed Central - PubMed

Affiliation: Dept. of Cellular and Molecular Biology, Islamic Azad University Babol-Branch, Iran.

ABSTRACT

Background: Adult worms of Orientobilharzia turkestanicum live in the portal veins, or intestinal veins of cattle, sheep, goat and many other mammals causing orientobilharziasis. Orientobilharziasis causes significant economic losses to livestock industry of Iran. However, there is limited information about genotypes of O. turkestanicum in Iran.

Methods: In this study, 30 isolates of O. turkestanicum obtained from sheep were characterized by sequencing mitochondrial cytochrome c oxidase subunit 1 (cox1) and nicotinamide adenine dinucleotide dehydrogenase subunit 1 (nad1) gene. The mitochondrial cox1 and nad1 DNA were amplified by polymerase chain reaction (PCR) and then sequenced and compared with O. turkestanicum and that of other members of the Schistosomatidae available in Gen-Bank(™).

Results: Phylogenetic relationships between them were re-constructed using the maximum parsimony method. Phylogenetic analyses done in present study placed O. turkestanicum within the Schistosoma genus, and indicates that O. turkestanicum was phylogenetically closer to the African schistosome group than to the Asian schistosome group.

Conclusion: Comparison of nad1 and cox1 sequences of O. turkestanicum obtained in this study with corresponding sequences available in Genbank(™) revealed some sequence variations and provided evidence for presence of microvarients in Iran.

No MeSH data available.


phylogenic relationship between the isolated sequences and various members of Schistosomatidae by maximum parsimony method based on the cox1 sequences. Fasciola hepatica (AJ628039) used as the out group. The various members of the Schistosomatidae that used were consist of Schistosoma bovis (AY157212), Schistosoma intercalatum (AY157208), Schistosoma leiperi (AY157207), Schistosoma haematobium (AY157209), Schistosoma mattheei (AY157211), Schistosoma margrebowiei (AY157206), Schistosoma mansoni (U82265), Schistosoma rodhaini (AY157202), Orientobilharzia turkestanicum (AY157200), Schistosoma malayensis (AY157198), Schistosoma mekongi (AY157199), Schistosoma japonicum (U82264), Gigantobilharzia huronensis (AY157188), Trichobilharzia regenti (AY157190), Dendritobilharzia pulverulenta (AY157187), Bilharziella polonica (AY157186), Austrobilharzia variglandis (AY157196), Orientobilharzia canaliculata (AY157194)
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F2b: phylogenic relationship between the isolated sequences and various members of Schistosomatidae by maximum parsimony method based on the cox1 sequences. Fasciola hepatica (AJ628039) used as the out group. The various members of the Schistosomatidae that used were consist of Schistosoma bovis (AY157212), Schistosoma intercalatum (AY157208), Schistosoma leiperi (AY157207), Schistosoma haematobium (AY157209), Schistosoma mattheei (AY157211), Schistosoma margrebowiei (AY157206), Schistosoma mansoni (U82265), Schistosoma rodhaini (AY157202), Orientobilharzia turkestanicum (AY157200), Schistosoma malayensis (AY157198), Schistosoma mekongi (AY157199), Schistosoma japonicum (U82264), Gigantobilharzia huronensis (AY157188), Trichobilharzia regenti (AY157190), Dendritobilharzia pulverulenta (AY157187), Bilharziella polonica (AY157186), Austrobilharzia variglandis (AY157196), Orientobilharzia canaliculata (AY157194)


Genetic Identification of Orientobilharzia turkestanicum from Sheep Isolates in Iran.

Tabaripour R, Youssefi MR, Tabaripour R - Iran J Parasitol (2015 Jan-Mar)

phylogenic relationship between the isolated sequences and various members of Schistosomatidae by maximum parsimony method based on the cox1 sequences. Fasciola hepatica (AJ628039) used as the out group. The various members of the Schistosomatidae that used were consist of Schistosoma bovis (AY157212), Schistosoma intercalatum (AY157208), Schistosoma leiperi (AY157207), Schistosoma haematobium (AY157209), Schistosoma mattheei (AY157211), Schistosoma margrebowiei (AY157206), Schistosoma mansoni (U82265), Schistosoma rodhaini (AY157202), Orientobilharzia turkestanicum (AY157200), Schistosoma malayensis (AY157198), Schistosoma mekongi (AY157199), Schistosoma japonicum (U82264), Gigantobilharzia huronensis (AY157188), Trichobilharzia regenti (AY157190), Dendritobilharzia pulverulenta (AY157187), Bilharziella polonica (AY157186), Austrobilharzia variglandis (AY157196), Orientobilharzia canaliculata (AY157194)
© Copyright Policy
Related In: Results  -  Collection

Show All Figures
getmorefigures.php?uid=PMC4403541&req=5

F2b: phylogenic relationship between the isolated sequences and various members of Schistosomatidae by maximum parsimony method based on the cox1 sequences. Fasciola hepatica (AJ628039) used as the out group. The various members of the Schistosomatidae that used were consist of Schistosoma bovis (AY157212), Schistosoma intercalatum (AY157208), Schistosoma leiperi (AY157207), Schistosoma haematobium (AY157209), Schistosoma mattheei (AY157211), Schistosoma margrebowiei (AY157206), Schistosoma mansoni (U82265), Schistosoma rodhaini (AY157202), Orientobilharzia turkestanicum (AY157200), Schistosoma malayensis (AY157198), Schistosoma mekongi (AY157199), Schistosoma japonicum (U82264), Gigantobilharzia huronensis (AY157188), Trichobilharzia regenti (AY157190), Dendritobilharzia pulverulenta (AY157187), Bilharziella polonica (AY157186), Austrobilharzia variglandis (AY157196), Orientobilharzia canaliculata (AY157194)
Bottom Line: Orientobilharziasis causes significant economic losses to livestock industry of Iran.The mitochondrial cox1 and nad1 DNA were amplified by polymerase chain reaction (PCR) and then sequenced and compared with O. turkestanicum and that of other members of the Schistosomatidae available in Gen-Bank(™).Comparison of nad1 and cox1 sequences of O. turkestanicum obtained in this study with corresponding sequences available in Genbank(™) revealed some sequence variations and provided evidence for presence of microvarients in Iran.

View Article: PubMed Central - PubMed

Affiliation: Dept. of Cellular and Molecular Biology, Islamic Azad University Babol-Branch, Iran.

ABSTRACT

Background: Adult worms of Orientobilharzia turkestanicum live in the portal veins, or intestinal veins of cattle, sheep, goat and many other mammals causing orientobilharziasis. Orientobilharziasis causes significant economic losses to livestock industry of Iran. However, there is limited information about genotypes of O. turkestanicum in Iran.

Methods: In this study, 30 isolates of O. turkestanicum obtained from sheep were characterized by sequencing mitochondrial cytochrome c oxidase subunit 1 (cox1) and nicotinamide adenine dinucleotide dehydrogenase subunit 1 (nad1) gene. The mitochondrial cox1 and nad1 DNA were amplified by polymerase chain reaction (PCR) and then sequenced and compared with O. turkestanicum and that of other members of the Schistosomatidae available in Gen-Bank(™).

Results: Phylogenetic relationships between them were re-constructed using the maximum parsimony method. Phylogenetic analyses done in present study placed O. turkestanicum within the Schistosoma genus, and indicates that O. turkestanicum was phylogenetically closer to the African schistosome group than to the Asian schistosome group.

Conclusion: Comparison of nad1 and cox1 sequences of O. turkestanicum obtained in this study with corresponding sequences available in Genbank(™) revealed some sequence variations and provided evidence for presence of microvarients in Iran.

No MeSH data available.