Limits...
Evaluation of Dot Immunogold Filtration Assay (DIGFA) By Recombinant Protein EPC1 for Anti- Echinococcus granulosus IgG Antibody.

Kordafshari S, Hosseini SH, Jalousian F, Rajabibazl M, Jones M, Etebar F - Iran J Parasitol (2015 Jan-Mar)

Bottom Line: Colloidal gold was prepared by controlled reduction of a boiling solution of chloroauric acid (H [AuCl4]) with sodium citrate and labeled with recombinant EPC1.Gold labeled antigen, showed a dark purple dot with agglutination particles in positive sera and light purple dot without agglutination in negative sera.Among 30 serum samples, 23 were positive and 7 were negative with DIGFA and 24 were positive, 6 were negative with ELISA.

View Article: PubMed Central - PubMed

Affiliation: Dept. of Parasitology, School of Veterinary Medicine, Tehran University, Tehran, Iran.

ABSTRACT

Background: Cystic echinococcosis is an important zoonosis caused by the Echinococcus granulosus with a substantial impact in human and animal health in endemic areas. The purpose of the present study was serodiagnosis optimizing of dog echinococcosis in order to achieve a rapid diagnostic method.

Methods: Eight dogs were challenged with protoscoleces in order to have positive echinococcosis serum and 2 two-month old puppies were used as uninfected controls. Colloidal gold was prepared by controlled reduction of a boiling solution of chloroauric acid (H [AuCl4]) with sodium citrate and labeled with recombinant EPC1. Dot immunogold filtration assay (DIGFA) was developed by coating rEPC1 labeled colloidal gold on nitrocellulose membrane. The canine sera, taken three times including, 15, 28 and 35 days post infection were tested. A total of 30 serum samples including 24 sera from 8 infected dogs and 6 sera from 2 puppies were comparatively detected with both DIGFA and ELISA.

Results: Gold labeled antigen, showed a dark purple dot with agglutination particles in positive sera and light purple dot without agglutination in negative sera. Among 30 serum samples, 23 were positive and 7 were negative with DIGFA and 24 were positive, 6 were negative with ELISA.

Conclusion: DIGFA as a rapid and simple procedure could be utilized in quickly diagnosis of echinococcosis.

No MeSH data available.


Related in: MedlinePlus

–Some of the positive and negative results of DIGFA with dogs positive and negative sera (dilution of 1:50) in different days post infection. Round, dark purple dot at the center of the square with agglutination particles can be seen in positive samples. In negative samples, a color of light purple is seen which is belong to the colloidal gold
© Copyright Policy
Related In: Results  -  Collection


getmorefigures.php?uid=PMC4403537&req=5

Figure 6: –Some of the positive and negative results of DIGFA with dogs positive and negative sera (dilution of 1:50) in different days post infection. Round, dark purple dot at the center of the square with agglutination particles can be seen in positive samples. In negative samples, a color of light purple is seen which is belong to the colloidal gold

Mentions: The intensity of the dot color was judged with naked eye (Fig. 6). Positive results were decided by the appearance of regular, round, dark purple dot at the center of the square with agglutination particles whereas the negative squares appeared light purple as we expected since the rEPC1 became purple after nano gold labeling process.


Evaluation of Dot Immunogold Filtration Assay (DIGFA) By Recombinant Protein EPC1 for Anti- Echinococcus granulosus IgG Antibody.

Kordafshari S, Hosseini SH, Jalousian F, Rajabibazl M, Jones M, Etebar F - Iran J Parasitol (2015 Jan-Mar)

–Some of the positive and negative results of DIGFA with dogs positive and negative sera (dilution of 1:50) in different days post infection. Round, dark purple dot at the center of the square with agglutination particles can be seen in positive samples. In negative samples, a color of light purple is seen which is belong to the colloidal gold
© Copyright Policy
Related In: Results  -  Collection

Show All Figures
getmorefigures.php?uid=PMC4403537&req=5

Figure 6: –Some of the positive and negative results of DIGFA with dogs positive and negative sera (dilution of 1:50) in different days post infection. Round, dark purple dot at the center of the square with agglutination particles can be seen in positive samples. In negative samples, a color of light purple is seen which is belong to the colloidal gold
Mentions: The intensity of the dot color was judged with naked eye (Fig. 6). Positive results were decided by the appearance of regular, round, dark purple dot at the center of the square with agglutination particles whereas the negative squares appeared light purple as we expected since the rEPC1 became purple after nano gold labeling process.

Bottom Line: Colloidal gold was prepared by controlled reduction of a boiling solution of chloroauric acid (H [AuCl4]) with sodium citrate and labeled with recombinant EPC1.Gold labeled antigen, showed a dark purple dot with agglutination particles in positive sera and light purple dot without agglutination in negative sera.Among 30 serum samples, 23 were positive and 7 were negative with DIGFA and 24 were positive, 6 were negative with ELISA.

View Article: PubMed Central - PubMed

Affiliation: Dept. of Parasitology, School of Veterinary Medicine, Tehran University, Tehran, Iran.

ABSTRACT

Background: Cystic echinococcosis is an important zoonosis caused by the Echinococcus granulosus with a substantial impact in human and animal health in endemic areas. The purpose of the present study was serodiagnosis optimizing of dog echinococcosis in order to achieve a rapid diagnostic method.

Methods: Eight dogs were challenged with protoscoleces in order to have positive echinococcosis serum and 2 two-month old puppies were used as uninfected controls. Colloidal gold was prepared by controlled reduction of a boiling solution of chloroauric acid (H [AuCl4]) with sodium citrate and labeled with recombinant EPC1. Dot immunogold filtration assay (DIGFA) was developed by coating rEPC1 labeled colloidal gold on nitrocellulose membrane. The canine sera, taken three times including, 15, 28 and 35 days post infection were tested. A total of 30 serum samples including 24 sera from 8 infected dogs and 6 sera from 2 puppies were comparatively detected with both DIGFA and ELISA.

Results: Gold labeled antigen, showed a dark purple dot with agglutination particles in positive sera and light purple dot without agglutination in negative sera. Among 30 serum samples, 23 were positive and 7 were negative with DIGFA and 24 were positive, 6 were negative with ELISA.

Conclusion: DIGFA as a rapid and simple procedure could be utilized in quickly diagnosis of echinococcosis.

No MeSH data available.


Related in: MedlinePlus