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Trichostrongylus colubriformis: Possible Most Common Cause of Human Infection in Mazandaran Province, North of Iran.

Gholami S, Babamahmoodi F, Abedian R, Sharif M, Shahbazi A, Pagheh A, Fakhar M - Iran J Parasitol (2015 Jan-Mar)

Bottom Line: The aim of present study was to identify Trichostrongylus spp. among human population using excreted egg specimens, by the molecular method, in Mazandaran Province, northern Iran.Overall, 33 positive fecal specimens were randomly sampled and examined.A total of 33 positive fecal specimens, 29(78.9%), 4(12.1%) were found T. colubriformis and T. axei respectively.

View Article: PubMed Central - PubMed

Affiliation: Molecular and Cell Biology Research Center, Mazandaran University of Medical Sciences, Sari, Iran.

ABSTRACT

Background: Infection with Trichostrongylus spp. is common among human and herbivorous in most parts of Iran, especially in southern and northern areas. The aim of present study was to identify Trichostrongylus spp. among human population using excreted egg specimens, by the molecular method, in Mazandaran Province, northern Iran.

Methods: Overall, 33 positive fecal specimens were randomly sampled and examined. PCR amplification of ITS2-rDNA region was performed on the isolated egg and then a restriction fragment length polymorphism (RFLP) profile was considered to discriminate of Trichostrongylus spp.

Results: A total of 33 positive fecal specimens, 29(78.9%), 4(12.1%) were found T. colubriformis and T. axei respectively. Our data appear the molecular evidence of both human T. colubriformis and T. axei infections in North of Iran.

Conclusion: T. colubriformis was the probable most common zoonotic species causing human trichostrongylosis infection in the area.

No MeSH data available.


Related in: MedlinePlus

Trichostrongylus species identified by PCRRFLP of ITS2 Region of rDNA gene using Hinf1enzyme. Lane 1–2 T. colubriformis (238bp, 90bp), Lane 3–4 using Dral enzyme T. axei (218bp, 110bp), M: 100bp DNA marker ).
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Figure 3: Trichostrongylus species identified by PCRRFLP of ITS2 Region of rDNA gene using Hinf1enzyme. Lane 1–2 T. colubriformis (238bp, 90bp), Lane 3–4 using Dral enzyme T. axei (218bp, 110bp), M: 100bp DNA marker ).

Mentions: Overall, 33 egg isolates based on the PCRRFLP, two species including T. colubriformis 29(78.9%) and T.axei4 (12.1%) were identified. Two different fragments were produced with Hinfl enzyme in T. colubriformis (about 90bp and 238bp, respectively). Similarly restriction with DraI enzyme produced two different fragment size in the PCR product of T.colubriformis was 238, 90 bp and T.axei 218,110 bp respectively. However, there were found differences between species in their RFLP-ITS2 patterns (Fig. 3). The unrestricted PCR products were of a similar size (about 330 bp) for all 2 species (Fig. 1). However, their restriction patterns were different in these species (Fig 2. and Fig 3.). Conversely, the difference between the two species in their restriction patterns is observed.


Trichostrongylus colubriformis: Possible Most Common Cause of Human Infection in Mazandaran Province, North of Iran.

Gholami S, Babamahmoodi F, Abedian R, Sharif M, Shahbazi A, Pagheh A, Fakhar M - Iran J Parasitol (2015 Jan-Mar)

Trichostrongylus species identified by PCRRFLP of ITS2 Region of rDNA gene using Hinf1enzyme. Lane 1–2 T. colubriformis (238bp, 90bp), Lane 3–4 using Dral enzyme T. axei (218bp, 110bp), M: 100bp DNA marker ).
© Copyright Policy
Related In: Results  -  Collection

Show All Figures
getmorefigures.php?uid=PMC4403529&req=5

Figure 3: Trichostrongylus species identified by PCRRFLP of ITS2 Region of rDNA gene using Hinf1enzyme. Lane 1–2 T. colubriformis (238bp, 90bp), Lane 3–4 using Dral enzyme T. axei (218bp, 110bp), M: 100bp DNA marker ).
Mentions: Overall, 33 egg isolates based on the PCRRFLP, two species including T. colubriformis 29(78.9%) and T.axei4 (12.1%) were identified. Two different fragments were produced with Hinfl enzyme in T. colubriformis (about 90bp and 238bp, respectively). Similarly restriction with DraI enzyme produced two different fragment size in the PCR product of T.colubriformis was 238, 90 bp and T.axei 218,110 bp respectively. However, there were found differences between species in their RFLP-ITS2 patterns (Fig. 3). The unrestricted PCR products were of a similar size (about 330 bp) for all 2 species (Fig. 1). However, their restriction patterns were different in these species (Fig 2. and Fig 3.). Conversely, the difference between the two species in their restriction patterns is observed.

Bottom Line: The aim of present study was to identify Trichostrongylus spp. among human population using excreted egg specimens, by the molecular method, in Mazandaran Province, northern Iran.Overall, 33 positive fecal specimens were randomly sampled and examined.A total of 33 positive fecal specimens, 29(78.9%), 4(12.1%) were found T. colubriformis and T. axei respectively.

View Article: PubMed Central - PubMed

Affiliation: Molecular and Cell Biology Research Center, Mazandaran University of Medical Sciences, Sari, Iran.

ABSTRACT

Background: Infection with Trichostrongylus spp. is common among human and herbivorous in most parts of Iran, especially in southern and northern areas. The aim of present study was to identify Trichostrongylus spp. among human population using excreted egg specimens, by the molecular method, in Mazandaran Province, northern Iran.

Methods: Overall, 33 positive fecal specimens were randomly sampled and examined. PCR amplification of ITS2-rDNA region was performed on the isolated egg and then a restriction fragment length polymorphism (RFLP) profile was considered to discriminate of Trichostrongylus spp.

Results: A total of 33 positive fecal specimens, 29(78.9%), 4(12.1%) were found T. colubriformis and T. axei respectively. Our data appear the molecular evidence of both human T. colubriformis and T. axei infections in North of Iran.

Conclusion: T. colubriformis was the probable most common zoonotic species causing human trichostrongylosis infection in the area.

No MeSH data available.


Related in: MedlinePlus