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Experimental Life Cycle of Hypoderaeum conoideum (Block, 1872) Diez, 1909(Trematoda: Echinostomatidae) Parasite from the North of Iran.

Azizi H, Farahnak A, Mobedi I, Molaei Rad M - Iran J Parasitol (2015 Jan-Mar)

Bottom Line: Ducks presented worm eggs in feces after 10-15 days post-infection.H. conoideum cercariae and adult worm are described.This is the first report of the different stages of the experimental life cycle of this parasite in Iran.

View Article: PubMed Central - PubMed

Affiliation: Dept. of Parasitology and Mycology, School of Public Health, Tehran University of Medical Sciences, Tehran, Iran.

ABSTRACT

Background: Human Echinostomiasis is an intestinal disease caused by the members of family Echinostomatidae parasites. The aim of present research was to identify echinostomatidae cercariae emitted by Lymnaea palustris snails from Mazandaran province in the north of Iran based on the morphological and morphometrical characteristics of the different stages of experimental parasite life cycle.

Methods: Echinostomatidae cercariae were collected from L. palustris (Gastropoda: Lymnaeidae) of the north of Iran. To collect metacercaria, 50 healthy snails were infected with cercariae experimentally (50 cercariae for each). To obtain the adult stage, 9 laboratory animals (3 ducks, 2 rats, 2 mice and 2 quails) were fed with 60 metacercaria for each. To identify parasite, the different stages of worm were examined using light microscope and then the figures were draw under camera Lucida microscope and measures were determined.

Results: Averagely, 15metacercaria were obtained from each snail that had been previously exposed with cercariae. Ducks presented worm eggs in feces after 10-15 days post-infection. Intestinal worms were collected and identified as Hypoderaeum conoideum on the bases of figures and measures of cephalic collar, the number of collar spine, suckers diameter ratio, testes arrangement, etc.

Conclusion: H. conoideum cercariae and adult worm are described. This is the first report of the different stages of the experimental life cycle of this parasite in Iran.

No MeSH data available.


Related in: MedlinePlus

A, Drawing picture of Hypoderaeum conoideum adult parasite in ventral view sucker; B, Drawing picture of Hypoderaeum conoideum adult parasite with spined collar arrangement in anterior portion; C, Photo Picture of Anterior portion of Hypoderaeum conoideum adult parasite with (1- collar and 2- cirrus sac) ; D, Photo Picture of Posterior portion of Hypoderaeum conoideum adult parasite with testis and excretory duct; E, Photo Picture of Hypoderaeum conoideum egg; F, Photo Picture of Miracidium in Hypoderaeum conoideum egg
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Figure 3: A, Drawing picture of Hypoderaeum conoideum adult parasite in ventral view sucker; B, Drawing picture of Hypoderaeum conoideum adult parasite with spined collar arrangement in anterior portion; C, Photo Picture of Anterior portion of Hypoderaeum conoideum adult parasite with (1- collar and 2- cirrus sac) ; D, Photo Picture of Posterior portion of Hypoderaeum conoideum adult parasite with testis and excretory duct; E, Photo Picture of Hypoderaeum conoideum egg; F, Photo Picture of Miracidium in Hypoderaeum conoideum egg

Mentions: Stool examination revealed parasite egg (100×50 micron) in ducks on the 10th post infection day. From three infected ducks (one duck dead during 3rd day infectivity), after 10–15 days, 15 hypoderaeum were collected from small intestine. The body of H. conoideum is elongated. The anterior part of body covered with minute spines extending to half-point of ventral sucker on ventral side and to half-point to the mid-posterior on the dorsal side. The collar is poorly developed with 52 spines in two rows (on lateral side two rows exist is more sensitive). Ventral sucker is placed in the first sixth of the body and is bigger than oral sucker and sucker diameter ratio is 1:4. Digestive system includes short and muscular pharynx. Esophagus bifurcating is in front of the ventral sucker. The two intestinal cecae extend to near the posterior end of the body. The cirrus and genital sac are well developed. Testes are arranged in tandem and are slightly lobulated that contiguously located in the posterior half of the body. The cirrus-sac is club-shaped reaching back almost to the posterior margin of the ventral sucker. Ovary is located in front of the testes. Linear uterus is located between oviduct and ventral sucker and containing many eggs. Vitellarium follicular is laterally extending from about just behind the posterior end of ventral sucker to near the posterior extremity (Fig.3). Table 2 shows the measures of adult worm were obtained from ducks and Table 3 shows the Figures abbreviations. Other animals (Balb/c, Rattus norvegicus and Coturnix ypsilophora) fed with metacercariae were parasite free in autopsy after 1–2 months post infection.


Experimental Life Cycle of Hypoderaeum conoideum (Block, 1872) Diez, 1909(Trematoda: Echinostomatidae) Parasite from the North of Iran.

Azizi H, Farahnak A, Mobedi I, Molaei Rad M - Iran J Parasitol (2015 Jan-Mar)

A, Drawing picture of Hypoderaeum conoideum adult parasite in ventral view sucker; B, Drawing picture of Hypoderaeum conoideum adult parasite with spined collar arrangement in anterior portion; C, Photo Picture of Anterior portion of Hypoderaeum conoideum adult parasite with (1- collar and 2- cirrus sac) ; D, Photo Picture of Posterior portion of Hypoderaeum conoideum adult parasite with testis and excretory duct; E, Photo Picture of Hypoderaeum conoideum egg; F, Photo Picture of Miracidium in Hypoderaeum conoideum egg
© Copyright Policy
Related In: Results  -  Collection

Show All Figures
getmorefigures.php?uid=PMC4403528&req=5

Figure 3: A, Drawing picture of Hypoderaeum conoideum adult parasite in ventral view sucker; B, Drawing picture of Hypoderaeum conoideum adult parasite with spined collar arrangement in anterior portion; C, Photo Picture of Anterior portion of Hypoderaeum conoideum adult parasite with (1- collar and 2- cirrus sac) ; D, Photo Picture of Posterior portion of Hypoderaeum conoideum adult parasite with testis and excretory duct; E, Photo Picture of Hypoderaeum conoideum egg; F, Photo Picture of Miracidium in Hypoderaeum conoideum egg
Mentions: Stool examination revealed parasite egg (100×50 micron) in ducks on the 10th post infection day. From three infected ducks (one duck dead during 3rd day infectivity), after 10–15 days, 15 hypoderaeum were collected from small intestine. The body of H. conoideum is elongated. The anterior part of body covered with minute spines extending to half-point of ventral sucker on ventral side and to half-point to the mid-posterior on the dorsal side. The collar is poorly developed with 52 spines in two rows (on lateral side two rows exist is more sensitive). Ventral sucker is placed in the first sixth of the body and is bigger than oral sucker and sucker diameter ratio is 1:4. Digestive system includes short and muscular pharynx. Esophagus bifurcating is in front of the ventral sucker. The two intestinal cecae extend to near the posterior end of the body. The cirrus and genital sac are well developed. Testes are arranged in tandem and are slightly lobulated that contiguously located in the posterior half of the body. The cirrus-sac is club-shaped reaching back almost to the posterior margin of the ventral sucker. Ovary is located in front of the testes. Linear uterus is located between oviduct and ventral sucker and containing many eggs. Vitellarium follicular is laterally extending from about just behind the posterior end of ventral sucker to near the posterior extremity (Fig.3). Table 2 shows the measures of adult worm were obtained from ducks and Table 3 shows the Figures abbreviations. Other animals (Balb/c, Rattus norvegicus and Coturnix ypsilophora) fed with metacercariae were parasite free in autopsy after 1–2 months post infection.

Bottom Line: Ducks presented worm eggs in feces after 10-15 days post-infection.H. conoideum cercariae and adult worm are described.This is the first report of the different stages of the experimental life cycle of this parasite in Iran.

View Article: PubMed Central - PubMed

Affiliation: Dept. of Parasitology and Mycology, School of Public Health, Tehran University of Medical Sciences, Tehran, Iran.

ABSTRACT

Background: Human Echinostomiasis is an intestinal disease caused by the members of family Echinostomatidae parasites. The aim of present research was to identify echinostomatidae cercariae emitted by Lymnaea palustris snails from Mazandaran province in the north of Iran based on the morphological and morphometrical characteristics of the different stages of experimental parasite life cycle.

Methods: Echinostomatidae cercariae were collected from L. palustris (Gastropoda: Lymnaeidae) of the north of Iran. To collect metacercaria, 50 healthy snails were infected with cercariae experimentally (50 cercariae for each). To obtain the adult stage, 9 laboratory animals (3 ducks, 2 rats, 2 mice and 2 quails) were fed with 60 metacercaria for each. To identify parasite, the different stages of worm were examined using light microscope and then the figures were draw under camera Lucida microscope and measures were determined.

Results: Averagely, 15metacercaria were obtained from each snail that had been previously exposed with cercariae. Ducks presented worm eggs in feces after 10-15 days post-infection. Intestinal worms were collected and identified as Hypoderaeum conoideum on the bases of figures and measures of cephalic collar, the number of collar spine, suckers diameter ratio, testes arrangement, etc.

Conclusion: H. conoideum cercariae and adult worm are described. This is the first report of the different stages of the experimental life cycle of this parasite in Iran.

No MeSH data available.


Related in: MedlinePlus