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Experimental Life Cycle of Hypoderaeum conoideum (Block, 1872) Diez, 1909(Trematoda: Echinostomatidae) Parasite from the North of Iran.

Azizi H, Farahnak A, Mobedi I, Molaei Rad M - Iran J Parasitol (2015 Jan-Mar)

Bottom Line: Ducks presented worm eggs in feces after 10-15 days post-infection.H. conoideum cercariae and adult worm are described.This is the first report of the different stages of the experimental life cycle of this parasite in Iran.

View Article: PubMed Central - PubMed

Affiliation: Dept. of Parasitology and Mycology, School of Public Health, Tehran University of Medical Sciences, Tehran, Iran.

ABSTRACT

Background: Human Echinostomiasis is an intestinal disease caused by the members of family Echinostomatidae parasites. The aim of present research was to identify echinostomatidae cercariae emitted by Lymnaea palustris snails from Mazandaran province in the north of Iran based on the morphological and morphometrical characteristics of the different stages of experimental parasite life cycle.

Methods: Echinostomatidae cercariae were collected from L. palustris (Gastropoda: Lymnaeidae) of the north of Iran. To collect metacercaria, 50 healthy snails were infected with cercariae experimentally (50 cercariae for each). To obtain the adult stage, 9 laboratory animals (3 ducks, 2 rats, 2 mice and 2 quails) were fed with 60 metacercaria for each. To identify parasite, the different stages of worm were examined using light microscope and then the figures were draw under camera Lucida microscope and measures were determined.

Results: Averagely, 15metacercaria were obtained from each snail that had been previously exposed with cercariae. Ducks presented worm eggs in feces after 10-15 days post-infection. Intestinal worms were collected and identified as Hypoderaeum conoideum on the bases of figures and measures of cephalic collar, the number of collar spine, suckers diameter ratio, testes arrangement, etc.

Conclusion: H. conoideum cercariae and adult worm are described. This is the first report of the different stages of the experimental life cycle of this parasite in Iran.

No MeSH data available.


Related in: MedlinePlus

A, Drawing picture of Hypoderaeum conoideum sucker; B, Drawing picture of Hypoderaeum conoideum spined collar arrangement; C, Photo picture of Hypoderaeum conoideum cercaria with simple loop 1) and coarse granules 2)
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Figure 1: A, Drawing picture of Hypoderaeum conoideum sucker; B, Drawing picture of Hypoderaeum conoideum spined collar arrangement; C, Photo picture of Hypoderaeum conoideum cercaria with simple loop 1) and coarse granules 2)

Mentions: The cercariae discharged intermittently throughout the day. They typically swim near the bottom of the vessel. They swim actively by vigorous movements of the tail and during swimming; the body is bent ventrally and contracted. The cercariae were survived for 24 hours. The cercaria has a weak-developed head collar bearing 52 inconspicuous spines that Dorsal-spines seem arranged in a single row. Remaining arranged in double row (Fig.1, B). Body surface armed with small spines arranged in transverse rows. Cuticle is bearing spines on anterior and lateral margins as far as level of acetabular and on posterior margin of body. Oral sucker circular, smaller than acetabulum and located terminally or subterminally. Acetabulum is highly prominent, situated posterior to middle of body. Digestive system consists of mouth, short prepharynx, an elongate pharynx and a large slender esophagus. Esophagus is divided just anterior to acetabulum into intestinal ceaca extending to posterior end of body. The totals of coarse excretory granules are 2×220 (Fig.1, c). At least 2×15 flame cells present but capillaries are very difficult to observe. Anterior margin of body with apertures of 6 narrow ducts which extend to posterior margin of oral sucker before becoming too difficult to trace. Paraoesophageal gland cells (n=16–17) are located around esophagus. Excretory bladder consists of two short, wide chambers that are connected by a narrow canal. Large primary excretory ducts arising from anterior chamber; ducts initially narrow but widening at level of acetabulum, connecting as far as pharynx and forming anterior loop by lateral to posterior margin of oral sucker before passing posteriorly as secondary ducts. Following a slightly sinuous course almost to posterior end of body, forming posterior loops at this level, and then passing interiorly to posterior level of acetabulum where bifurcation into anterior and posterior collected ducts located (Fig.1, A). Table 1 shows the measures of cercaiae obtained from L. palustris.


Experimental Life Cycle of Hypoderaeum conoideum (Block, 1872) Diez, 1909(Trematoda: Echinostomatidae) Parasite from the North of Iran.

Azizi H, Farahnak A, Mobedi I, Molaei Rad M - Iran J Parasitol (2015 Jan-Mar)

A, Drawing picture of Hypoderaeum conoideum sucker; B, Drawing picture of Hypoderaeum conoideum spined collar arrangement; C, Photo picture of Hypoderaeum conoideum cercaria with simple loop 1) and coarse granules 2)
© Copyright Policy
Related In: Results  -  Collection

Show All Figures
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Figure 1: A, Drawing picture of Hypoderaeum conoideum sucker; B, Drawing picture of Hypoderaeum conoideum spined collar arrangement; C, Photo picture of Hypoderaeum conoideum cercaria with simple loop 1) and coarse granules 2)
Mentions: The cercariae discharged intermittently throughout the day. They typically swim near the bottom of the vessel. They swim actively by vigorous movements of the tail and during swimming; the body is bent ventrally and contracted. The cercariae were survived for 24 hours. The cercaria has a weak-developed head collar bearing 52 inconspicuous spines that Dorsal-spines seem arranged in a single row. Remaining arranged in double row (Fig.1, B). Body surface armed with small spines arranged in transverse rows. Cuticle is bearing spines on anterior and lateral margins as far as level of acetabular and on posterior margin of body. Oral sucker circular, smaller than acetabulum and located terminally or subterminally. Acetabulum is highly prominent, situated posterior to middle of body. Digestive system consists of mouth, short prepharynx, an elongate pharynx and a large slender esophagus. Esophagus is divided just anterior to acetabulum into intestinal ceaca extending to posterior end of body. The totals of coarse excretory granules are 2×220 (Fig.1, c). At least 2×15 flame cells present but capillaries are very difficult to observe. Anterior margin of body with apertures of 6 narrow ducts which extend to posterior margin of oral sucker before becoming too difficult to trace. Paraoesophageal gland cells (n=16–17) are located around esophagus. Excretory bladder consists of two short, wide chambers that are connected by a narrow canal. Large primary excretory ducts arising from anterior chamber; ducts initially narrow but widening at level of acetabulum, connecting as far as pharynx and forming anterior loop by lateral to posterior margin of oral sucker before passing posteriorly as secondary ducts. Following a slightly sinuous course almost to posterior end of body, forming posterior loops at this level, and then passing interiorly to posterior level of acetabulum where bifurcation into anterior and posterior collected ducts located (Fig.1, A). Table 1 shows the measures of cercaiae obtained from L. palustris.

Bottom Line: Ducks presented worm eggs in feces after 10-15 days post-infection.H. conoideum cercariae and adult worm are described.This is the first report of the different stages of the experimental life cycle of this parasite in Iran.

View Article: PubMed Central - PubMed

Affiliation: Dept. of Parasitology and Mycology, School of Public Health, Tehran University of Medical Sciences, Tehran, Iran.

ABSTRACT

Background: Human Echinostomiasis is an intestinal disease caused by the members of family Echinostomatidae parasites. The aim of present research was to identify echinostomatidae cercariae emitted by Lymnaea palustris snails from Mazandaran province in the north of Iran based on the morphological and morphometrical characteristics of the different stages of experimental parasite life cycle.

Methods: Echinostomatidae cercariae were collected from L. palustris (Gastropoda: Lymnaeidae) of the north of Iran. To collect metacercaria, 50 healthy snails were infected with cercariae experimentally (50 cercariae for each). To obtain the adult stage, 9 laboratory animals (3 ducks, 2 rats, 2 mice and 2 quails) were fed with 60 metacercaria for each. To identify parasite, the different stages of worm were examined using light microscope and then the figures were draw under camera Lucida microscope and measures were determined.

Results: Averagely, 15metacercaria were obtained from each snail that had been previously exposed with cercariae. Ducks presented worm eggs in feces after 10-15 days post-infection. Intestinal worms were collected and identified as Hypoderaeum conoideum on the bases of figures and measures of cephalic collar, the number of collar spine, suckers diameter ratio, testes arrangement, etc.

Conclusion: H. conoideum cercariae and adult worm are described. This is the first report of the different stages of the experimental life cycle of this parasite in Iran.

No MeSH data available.


Related in: MedlinePlus