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pH levels drive bacterial community structure in sediments of the Qiantang River as determined by 454 pyrosequencing.

Liu S, Ren H, Shen L, Lou L, Tian G, Zheng P, Hu B - Front Microbiol (2015)

Bottom Line: However, the effects of environmental factors on the bacterial community of this freshwater ecosystem have not been determined.Using a linear-regression analysis, the OTU numbers were significantly positively correlated with pH (r = 0.832, p < 0.05) and negatively correlated with nitrate concentration (r = -0.805, p < 0.05).The results indicated that pH (p < 0.05) and nitrate concentration (p < 0.05) were the most significant factors that determined the community distribution of sediment bacteria.

View Article: PubMed Central - PubMed

Affiliation: Department of Environmental Engineering, Zhejiang University Hangzhou, China.

ABSTRACT
The Qiantang River is a typical freshwater ecosystem that acts as an irreplaceable water source in Zhejiang Province in southeastern China. However, the effects of environmental factors on the bacterial community of this freshwater ecosystem have not been determined. In this study, seven sediment samples were collected along the river. Their bacterial communities were identified using 454 high-throughput sequencing, and the primary environmental factors responsible for shaping the community structure were analyzed. The number of bacterial operational taxonomic units (OTUs) ranged from 2637 to 3933. Using a linear-regression analysis, the OTU numbers were significantly positively correlated with pH (r = 0.832, p < 0.05) and negatively correlated with nitrate concentration (r = -0.805, p < 0.05). A redundancy analysis (RDA) was also performed to test the relationship between the environmental factors and bacterial community composition. The results indicated that pH (p < 0.05) and nitrate concentration (p < 0.05) were the most significant factors that determined the community distribution of sediment bacteria.

No MeSH data available.


The relative abundances of different phyla (A) and classes (B) in the seven sediment samples along the Qiantang River.
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Figure 1: The relative abundances of different phyla (A) and classes (B) in the seven sediment samples along the Qiantang River.

Mentions: At the phylum level, the top 10 phyla were selected, and the remaining sequences were assigned to a cluster named “the others” (Figure 1). The results revealed that Proteobacteria was the most abundant phylum across all seven sediment samples. The proportions of Proteobacteria in samples ranged from 33.99% at site MC to 46.70% at site JX. The other dominant phyla were Firmicutes (9.71–19.07%, averaging 14.97%), Bacteroidetes (10.59–20.08%, averaging 13.94%), Actinobacteria (6.82–10.85%, averaging 9.15%), and Chloroflexi (6.34–11.75%, averaging 8.33%). These five phyla dominated (83.50–87.45%) the bacterial communities in all seven sediment samples, and the following phyla were present at less than 3%: Nitrospirae (averaging 2.44%), Acidobacteria (averaging 2.23%), Cyanobacteria (averaging 2.11%), Spirochaetes (averaging 1.88%), and Verrucomicrobia (averaging 1.35%). The abundance of Nitrospirae-related sequences detected at site JX (4.78%) was much higher than at the other sites, and the Cyanobacteria-related sequences found at site XY (5.20%) were at least two times higher than at the other sites.


pH levels drive bacterial community structure in sediments of the Qiantang River as determined by 454 pyrosequencing.

Liu S, Ren H, Shen L, Lou L, Tian G, Zheng P, Hu B - Front Microbiol (2015)

The relative abundances of different phyla (A) and classes (B) in the seven sediment samples along the Qiantang River.
© Copyright Policy - open-access
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4403504&req=5

Figure 1: The relative abundances of different phyla (A) and classes (B) in the seven sediment samples along the Qiantang River.
Mentions: At the phylum level, the top 10 phyla were selected, and the remaining sequences were assigned to a cluster named “the others” (Figure 1). The results revealed that Proteobacteria was the most abundant phylum across all seven sediment samples. The proportions of Proteobacteria in samples ranged from 33.99% at site MC to 46.70% at site JX. The other dominant phyla were Firmicutes (9.71–19.07%, averaging 14.97%), Bacteroidetes (10.59–20.08%, averaging 13.94%), Actinobacteria (6.82–10.85%, averaging 9.15%), and Chloroflexi (6.34–11.75%, averaging 8.33%). These five phyla dominated (83.50–87.45%) the bacterial communities in all seven sediment samples, and the following phyla were present at less than 3%: Nitrospirae (averaging 2.44%), Acidobacteria (averaging 2.23%), Cyanobacteria (averaging 2.11%), Spirochaetes (averaging 1.88%), and Verrucomicrobia (averaging 1.35%). The abundance of Nitrospirae-related sequences detected at site JX (4.78%) was much higher than at the other sites, and the Cyanobacteria-related sequences found at site XY (5.20%) were at least two times higher than at the other sites.

Bottom Line: However, the effects of environmental factors on the bacterial community of this freshwater ecosystem have not been determined.Using a linear-regression analysis, the OTU numbers were significantly positively correlated with pH (r = 0.832, p < 0.05) and negatively correlated with nitrate concentration (r = -0.805, p < 0.05).The results indicated that pH (p < 0.05) and nitrate concentration (p < 0.05) were the most significant factors that determined the community distribution of sediment bacteria.

View Article: PubMed Central - PubMed

Affiliation: Department of Environmental Engineering, Zhejiang University Hangzhou, China.

ABSTRACT
The Qiantang River is a typical freshwater ecosystem that acts as an irreplaceable water source in Zhejiang Province in southeastern China. However, the effects of environmental factors on the bacterial community of this freshwater ecosystem have not been determined. In this study, seven sediment samples were collected along the river. Their bacterial communities were identified using 454 high-throughput sequencing, and the primary environmental factors responsible for shaping the community structure were analyzed. The number of bacterial operational taxonomic units (OTUs) ranged from 2637 to 3933. Using a linear-regression analysis, the OTU numbers were significantly positively correlated with pH (r = 0.832, p < 0.05) and negatively correlated with nitrate concentration (r = -0.805, p < 0.05). A redundancy analysis (RDA) was also performed to test the relationship between the environmental factors and bacterial community composition. The results indicated that pH (p < 0.05) and nitrate concentration (p < 0.05) were the most significant factors that determined the community distribution of sediment bacteria.

No MeSH data available.