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Local evolutionary patterns of human respiratory syncytial virus derived from whole-genome sequencing.

Agoti CN, Otieno JR, Munywoki PK, Mwihuri AG, Cane PA, Nokes DJ, Kellam P, Cotten M - J. Virol. (2015)

Bottom Line: The analysis of RSV full genomes, compared to subgenomic regions, provided more precise estimates of the RSV sequence changes and revealed important patterns of RSV genomic variation and global movement.The new RSV genomic sequences and the novel sequencing method reported here provide important data for understanding RSV transmission and vaccine development.Given the complex interplay between RSV A and RSV B infections, the existence of local RSV B evolution is an important factor in vaccine deployment.

View Article: PubMed Central - PubMed

Affiliation: KEMRI-Wellcome Trust Research Programme, Kilifi, Kenya.

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Related in: MedlinePlus

Comparison of RSVB genomes with identical G regions. Each panel represents a genome nucleotide alignment of RSVs that had identical G gene sequences. The G protein ORF portions of the genomes are highlighted gray across the panels and were identical. The vertical lines indicate where there are nucleotide substitutions occurring outside the G gene region between the genomes. The blue blocks indicate a gap in the sequence.
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Figure 3: Comparison of RSVB genomes with identical G regions. Each panel represents a genome nucleotide alignment of RSVs that had identical G gene sequences. The G protein ORF portions of the genomes are highlighted gray across the panels and were identical. The vertical lines indicate where there are nucleotide substitutions occurring outside the G gene region between the genomes. The blue blocks indicate a gap in the sequence.

Mentions: One motivation for developing full-genome methods was to increase the sensitivity for tracking RSV across short-term transmission chains. We asked if viruses identical in their G gene regions had differences elsewhere in their genomes. All RSV genomes (both GenBank or in the new data presented here) with identical G regions were identified, and the number of changes outside the G region were determined. Of 7 sets of viruses with identical G regions, all showed at least 1 but up to 9 nucleotide differences across the full genome (Fig. 3). This increased resolution will be important in future studies examining RSV household transmission patterns to identify who acquires infection from whom.


Local evolutionary patterns of human respiratory syncytial virus derived from whole-genome sequencing.

Agoti CN, Otieno JR, Munywoki PK, Mwihuri AG, Cane PA, Nokes DJ, Kellam P, Cotten M - J. Virol. (2015)

Comparison of RSVB genomes with identical G regions. Each panel represents a genome nucleotide alignment of RSVs that had identical G gene sequences. The G protein ORF portions of the genomes are highlighted gray across the panels and were identical. The vertical lines indicate where there are nucleotide substitutions occurring outside the G gene region between the genomes. The blue blocks indicate a gap in the sequence.
© Copyright Policy - open-access
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4403408&req=5

Figure 3: Comparison of RSVB genomes with identical G regions. Each panel represents a genome nucleotide alignment of RSVs that had identical G gene sequences. The G protein ORF portions of the genomes are highlighted gray across the panels and were identical. The vertical lines indicate where there are nucleotide substitutions occurring outside the G gene region between the genomes. The blue blocks indicate a gap in the sequence.
Mentions: One motivation for developing full-genome methods was to increase the sensitivity for tracking RSV across short-term transmission chains. We asked if viruses identical in their G gene regions had differences elsewhere in their genomes. All RSV genomes (both GenBank or in the new data presented here) with identical G regions were identified, and the number of changes outside the G region were determined. Of 7 sets of viruses with identical G regions, all showed at least 1 but up to 9 nucleotide differences across the full genome (Fig. 3). This increased resolution will be important in future studies examining RSV household transmission patterns to identify who acquires infection from whom.

Bottom Line: The analysis of RSV full genomes, compared to subgenomic regions, provided more precise estimates of the RSV sequence changes and revealed important patterns of RSV genomic variation and global movement.The new RSV genomic sequences and the novel sequencing method reported here provide important data for understanding RSV transmission and vaccine development.Given the complex interplay between RSV A and RSV B infections, the existence of local RSV B evolution is an important factor in vaccine deployment.

View Article: PubMed Central - PubMed

Affiliation: KEMRI-Wellcome Trust Research Programme, Kilifi, Kenya.

Show MeSH
Related in: MedlinePlus