Local evolutionary patterns of human respiratory syncytial virus derived from whole-genome sequencing.
Bottom Line: The analysis of RSV full genomes, compared to subgenomic regions, provided more precise estimates of the RSV sequence changes and revealed important patterns of RSV genomic variation and global movement.The new RSV genomic sequences and the novel sequencing method reported here provide important data for understanding RSV transmission and vaccine development.Given the complex interplay between RSV A and RSV B infections, the existence of local RSV B evolution is an important factor in vaccine deployment.
Affiliation: KEMRI-Wellcome Trust Research Programme, Kilifi, Kenya.Show MeSH
Related in: MedlinePlus
Mentions: One motivation for developing full-genome methods was to increase the sensitivity for tracking RSV across short-term transmission chains. We asked if viruses identical in their G gene regions had differences elsewhere in their genomes. All RSV genomes (both GenBank or in the new data presented here) with identical G regions were identified, and the number of changes outside the G region were determined. Of 7 sets of viruses with identical G regions, all showed at least 1 but up to 9 nucleotide differences across the full genome (Fig. 3). This increased resolution will be important in future studies examining RSV household transmission patterns to identify who acquires infection from whom.
Affiliation: KEMRI-Wellcome Trust Research Programme, Kilifi, Kenya.