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Rebuilding a realistic corticostriatal "social network" from dissociated cells.

Garcia-Munoz M, Taillefer E, Pnini R, Vickers C, Miller J, Arbuthnott GW - Front Syst Neurosci (2015)

Bottom Line: The activity of both areas can be recorded in multielectrode arrays or individual patch recordings from pairs of cells.Finally, corticostriatal connections can be severed acutely.This procedure enables determination of the importance of corticostriatal interaction in the resting pattern of activity.

View Article: PubMed Central - PubMed

Affiliation: Brain Mechanisms for Behaviour Unit, Okinawa Institute of Science and Technology Graduate University Okinawa, Japan.

ABSTRACT
Many of the methods available for the study of cortical influences on striatal neurons have serious problems. In vivo the connectivity is so complex that the study of input from an individual cortical neuron to a single striatal cell is nearly impossible. Mixed corticostriatal cultures develop many connections from striatal cells to cortical cells, in striking contrast to the fact that only connections from cortical cells to striatal cells are present in vivo. Furthermore, interneuron populations are over-represented in organotypic cultures. For these reasons, we have developed a method for growing cortical and striatal neurons in separated compartments that allows cortical neurons to innervate striatal cells in culture. The method works equally well for acutely dissociated or cryopreserved neurons and allows a number of manipulations that are not otherwise possible. Either cortical or striatal compartments can be transfected with channel rhodopsins. The activity of both areas can be recorded in multielectrode arrays or individual patch recordings from pairs of cells. Finally, corticostriatal connections can be severed acutely. This procedure enables determination of the importance of corticostriatal interaction in the resting pattern of activity. These cultures also facilitate development of sensitive analytical network methods to track connectivity.

No MeSH data available.


Related in: MedlinePlus

Correlations of regionally averaged potentials for all plates and the mutual information between electrodes for a representative experiment. (A) Average correlation between electrode activity averaged over the cortical region and electrode activity averaged over the striatal region (see Figure 4). (B) Only half of the electrode-electrode mutual information values are displayed since this quantity is symmetric. In medium there are many different z-scores across electrodes. In medium there are many different z-scores across electrodes. Left hand panel (Medium): lower left quadrant displays mutual information between cortical electrodes; upper right quadrant striatal electrode mutual information; lower right quadrant mutual information shared between the compartments. Right-hand panel (Cut) displays the dramatic fall in information within the striatum and its complete absence between regions. The slight increase in the cortical region is likely due to the intervening NMDA application (see panel A) rather than a direct consequence of the cut, but its source remains to be examined in further work. Details of similar analyses in other plates in this study are illustrated in Supplementary Figure 1.
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Figure 5: Correlations of regionally averaged potentials for all plates and the mutual information between electrodes for a representative experiment. (A) Average correlation between electrode activity averaged over the cortical region and electrode activity averaged over the striatal region (see Figure 4). (B) Only half of the electrode-electrode mutual information values are displayed since this quantity is symmetric. In medium there are many different z-scores across electrodes. In medium there are many different z-scores across electrodes. Left hand panel (Medium): lower left quadrant displays mutual information between cortical electrodes; upper right quadrant striatal electrode mutual information; lower right quadrant mutual information shared between the compartments. Right-hand panel (Cut) displays the dramatic fall in information within the striatum and its complete absence between regions. The slight increase in the cortical region is likely due to the intervening NMDA application (see panel A) rather than a direct consequence of the cut, but its source remains to be examined in further work. Details of similar analyses in other plates in this study are illustrated in Supplementary Figure 1.

Mentions: We computed correlations between all the electrodes. The correlation oefficient between cortical and striatal activity, calculated in 100 ms bins is shown in Figure 5. The differences between MEDIUM, NMDA and CUT are clear. A threshold value, was obtained by random data shuffling to ensure a p value of 0.005 for noise-level (Mao et al., 2001). When only those events whose activity exceeds θ are counted, it turns out that δcδs = 0 after CUT.


Rebuilding a realistic corticostriatal "social network" from dissociated cells.

Garcia-Munoz M, Taillefer E, Pnini R, Vickers C, Miller J, Arbuthnott GW - Front Syst Neurosci (2015)

Correlations of regionally averaged potentials for all plates and the mutual information between electrodes for a representative experiment. (A) Average correlation between electrode activity averaged over the cortical region and electrode activity averaged over the striatal region (see Figure 4). (B) Only half of the electrode-electrode mutual information values are displayed since this quantity is symmetric. In medium there are many different z-scores across electrodes. In medium there are many different z-scores across electrodes. Left hand panel (Medium): lower left quadrant displays mutual information between cortical electrodes; upper right quadrant striatal electrode mutual information; lower right quadrant mutual information shared between the compartments. Right-hand panel (Cut) displays the dramatic fall in information within the striatum and its complete absence between regions. The slight increase in the cortical region is likely due to the intervening NMDA application (see panel A) rather than a direct consequence of the cut, but its source remains to be examined in further work. Details of similar analyses in other plates in this study are illustrated in Supplementary Figure 1.
© Copyright Policy - open-access
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4403293&req=5

Figure 5: Correlations of regionally averaged potentials for all plates and the mutual information between electrodes for a representative experiment. (A) Average correlation between electrode activity averaged over the cortical region and electrode activity averaged over the striatal region (see Figure 4). (B) Only half of the electrode-electrode mutual information values are displayed since this quantity is symmetric. In medium there are many different z-scores across electrodes. In medium there are many different z-scores across electrodes. Left hand panel (Medium): lower left quadrant displays mutual information between cortical electrodes; upper right quadrant striatal electrode mutual information; lower right quadrant mutual information shared between the compartments. Right-hand panel (Cut) displays the dramatic fall in information within the striatum and its complete absence between regions. The slight increase in the cortical region is likely due to the intervening NMDA application (see panel A) rather than a direct consequence of the cut, but its source remains to be examined in further work. Details of similar analyses in other plates in this study are illustrated in Supplementary Figure 1.
Mentions: We computed correlations between all the electrodes. The correlation oefficient between cortical and striatal activity, calculated in 100 ms bins is shown in Figure 5. The differences between MEDIUM, NMDA and CUT are clear. A threshold value, was obtained by random data shuffling to ensure a p value of 0.005 for noise-level (Mao et al., 2001). When only those events whose activity exceeds θ are counted, it turns out that δcδs = 0 after CUT.

Bottom Line: The activity of both areas can be recorded in multielectrode arrays or individual patch recordings from pairs of cells.Finally, corticostriatal connections can be severed acutely.This procedure enables determination of the importance of corticostriatal interaction in the resting pattern of activity.

View Article: PubMed Central - PubMed

Affiliation: Brain Mechanisms for Behaviour Unit, Okinawa Institute of Science and Technology Graduate University Okinawa, Japan.

ABSTRACT
Many of the methods available for the study of cortical influences on striatal neurons have serious problems. In vivo the connectivity is so complex that the study of input from an individual cortical neuron to a single striatal cell is nearly impossible. Mixed corticostriatal cultures develop many connections from striatal cells to cortical cells, in striking contrast to the fact that only connections from cortical cells to striatal cells are present in vivo. Furthermore, interneuron populations are over-represented in organotypic cultures. For these reasons, we have developed a method for growing cortical and striatal neurons in separated compartments that allows cortical neurons to innervate striatal cells in culture. The method works equally well for acutely dissociated or cryopreserved neurons and allows a number of manipulations that are not otherwise possible. Either cortical or striatal compartments can be transfected with channel rhodopsins. The activity of both areas can be recorded in multielectrode arrays or individual patch recordings from pairs of cells. Finally, corticostriatal connections can be severed acutely. This procedure enables determination of the importance of corticostriatal interaction in the resting pattern of activity. These cultures also facilitate development of sensitive analytical network methods to track connectivity.

No MeSH data available.


Related in: MedlinePlus