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In-vitro and In-vivo Evaluation of Silymarin Nanoliposomes against Isolated Methicillin-resistant Staphylococcus aureus.

Faezizadeh Z, Gharib A, Godarzee M - Iran J Pharm Res (2015)

Bottom Line: The killing rate of free and nanoliposomal forms of silymarin were analyzed.The killing rate of silymarin-loaded nanoliposomes was higher than those of free silymarin.Topically treatment by silymarin in free and nanoliposomal forms resulted in almost 20 and 100% survival rates, respectively.

View Article: PubMed Central - PubMed

Affiliation: Department of Laboratory Sciences, Borujerd Branch, Islamic Azad University, Borujerd, Iran.

ABSTRACT
Staphylococcus aureus is an opportunistic pathogen and remains a common cause of burn wound infections. Different studies have shown that entrapment of plant-derived compounds into liposomes could increase their anti-Staphylococcus aureus activity. Silymarin is the bioactive extract from the known plant Silybum marianum L. The objective of this study was to evaluate efficacy of silymarin in free and nanoliposomal forms against isolated methicillin-resistant Staphylococcus aureus (MRSA) strain. Silymarin-loaded nanoliposomes were prepared by extrusion method. The minimum inhibitory concentrations (MICs) of silymarin in free and nanoliposomal forms against MRSA were determined by broth dilution method. The killing rate of free and nanoliposomal forms of silymarin were analyzed. Ultimately, in-vivo therapeutic efficacy of nanoliposomes in burned mice infected by isolated MRSA was examined. The MICs of free and nanoliposomal forms of silymarin against isolated strain were 500 and 125 mg/L, respectively. The killing rate of silymarin-loaded nanoliposomes was higher than those of free silymarin. Topically treatment by silymarin in free and nanoliposomal forms resulted in almost 20 and 100% survival rates, respectively. The results suggest that silymarin-loaded nanoliposomes may provide a basis for future treatment of MRSA infections.

No MeSH data available.


Related in: MedlinePlus

Chromatogram of silymarin analysis.
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Figure 1: Chromatogram of silymarin analysis.

Mentions: Recently, the use of plant-derived compounds to eliminate of MRSA has been widely investigated (24, 25). However, the main problems associated with application of some of these components are low water solubility and low bioavailability (12, 24). To overcome of these problems, the investigators were focused on entrapment of plant-derived compounds in drug carriers such as liposomes (13). Silymarin is a mixture of flavonolignans from the medical plant Silybum marianum (17). As shown in Figure 1, the silymarin content could be estimated from sum of its six lignans peak area.


In-vitro and In-vivo Evaluation of Silymarin Nanoliposomes against Isolated Methicillin-resistant Staphylococcus aureus.

Faezizadeh Z, Gharib A, Godarzee M - Iran J Pharm Res (2015)

Chromatogram of silymarin analysis.
© Copyright Policy
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4403081&req=5

Figure 1: Chromatogram of silymarin analysis.
Mentions: Recently, the use of plant-derived compounds to eliminate of MRSA has been widely investigated (24, 25). However, the main problems associated with application of some of these components are low water solubility and low bioavailability (12, 24). To overcome of these problems, the investigators were focused on entrapment of plant-derived compounds in drug carriers such as liposomes (13). Silymarin is a mixture of flavonolignans from the medical plant Silybum marianum (17). As shown in Figure 1, the silymarin content could be estimated from sum of its six lignans peak area.

Bottom Line: The killing rate of free and nanoliposomal forms of silymarin were analyzed.The killing rate of silymarin-loaded nanoliposomes was higher than those of free silymarin.Topically treatment by silymarin in free and nanoliposomal forms resulted in almost 20 and 100% survival rates, respectively.

View Article: PubMed Central - PubMed

Affiliation: Department of Laboratory Sciences, Borujerd Branch, Islamic Azad University, Borujerd, Iran.

ABSTRACT
Staphylococcus aureus is an opportunistic pathogen and remains a common cause of burn wound infections. Different studies have shown that entrapment of plant-derived compounds into liposomes could increase their anti-Staphylococcus aureus activity. Silymarin is the bioactive extract from the known plant Silybum marianum L. The objective of this study was to evaluate efficacy of silymarin in free and nanoliposomal forms against isolated methicillin-resistant Staphylococcus aureus (MRSA) strain. Silymarin-loaded nanoliposomes were prepared by extrusion method. The minimum inhibitory concentrations (MICs) of silymarin in free and nanoliposomal forms against MRSA were determined by broth dilution method. The killing rate of free and nanoliposomal forms of silymarin were analyzed. Ultimately, in-vivo therapeutic efficacy of nanoliposomes in burned mice infected by isolated MRSA was examined. The MICs of free and nanoliposomal forms of silymarin against isolated strain were 500 and 125 mg/L, respectively. The killing rate of silymarin-loaded nanoliposomes was higher than those of free silymarin. Topically treatment by silymarin in free and nanoliposomal forms resulted in almost 20 and 100% survival rates, respectively. The results suggest that silymarin-loaded nanoliposomes may provide a basis for future treatment of MRSA infections.

No MeSH data available.


Related in: MedlinePlus