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The Protective Effect of Sodium Ferulate and Oxymatrine Combination on Paraquat-induced Lung Injury.

Wang W, Pei X, Xu M, Sun S, Zhang C, Mu K, Liu Z - Iran J Pharm Res (2015)

Bottom Line: Also in SF and OMT combination treatment groups of rats, the increased lung W/D ratio and histopathological score induced by PQ injection were significantly decreased; the levels of CRP, IL-6, NF-κB and MDA in serum and lung homogenate were significantly decreased; the SOD activities in serum and lung homogenate were improved.These results suggested that SF and OMT combination had an obvious protective effect on PQ-induced lung injury.The anti-inflammatory and antioxidant effect might be involved in the mechanism.

View Article: PubMed Central - PubMed

Affiliation: School of Pharmacy, Yantai University, No. 30, Qingquan Road, Laishan District, Yantai, Shandong, China.

ABSTRACT
Experimental evidence suggested that sodium ferulate (SF) and oxymatrine (OMT) combination had synergistic anti-inflammatory and antioxidant effects. We hypothesized that SF and OMT combination treatment might have protective effects on paraquat-induced acute lung injury. In our study, the Swiss mice were randomly divided into seven groups, including control, paraquat (PQ), SF (6.2 mg/Kg/day); OMT (13.8 mg/Kg/day) and three SF+OMT groups (3.1 + 6.9; 6.2 + 13.8 and 12.3 + 27.7 mg/Kg/day). The mortality and death time were monitored. Sprague-Dawley rats were randomly divided into seven groups including control, PQ, SF (3.1 mg/Kg/day); OMT (6.9 mg/Kg/day) and three SF+OMT groups (1.6 + 3.4; 3.1 + 6.9 and 6.2 + 13.8 mg/Kg/day). The lung wet/dry weight (W/D) ratio, lung histopathologic changes, C-reactive protein (CRP), interleukin-6 (IL-6), nuclear factor κB (NF-κB), malondialdehyde (MDA) and superoxidase dismutase (SOD) were analysed. Compared with PQ group, the mortality significantly decreased and the death time prolonged in SF and OMT combination treatment groups of mice. Also in SF and OMT combination treatment groups of rats, the increased lung W/D ratio and histopathological score induced by PQ injection were significantly decreased; the levels of CRP, IL-6, NF-κB and MDA in serum and lung homogenate were significantly decreased; the SOD activities in serum and lung homogenate were improved. These results suggested that SF and OMT combination had an obvious protective effect on PQ-induced lung injury. The anti-inflammatory and antioxidant effect might be involved in the mechanism.

No MeSH data available.


Related in: MedlinePlus

Effects of SF and OMT combination on the levels of CRP (a), IL-6 (b), NF-κB (c) in the lung homogenate of the PQ-intoxication rats. CON, PQ, SF and OMT represent the control group, paraquat group, sodium ferulate group (3.1 mg/Kg/day) and oxymatrine group (6.9 mg/Kg/day), respectively. SF+OMT represents sodium ferulate and oxymatrine combination treatment groups, and the 1.6 + 3.4, 3.1 + 6.9 and 6.2 + 13.8 respectively represent drug administration doses of SF + OMT in the low dose group, middle dose group and high dose group. Data are expressed as means ± SEM, n = 10 in each group. ##P < 0.01 compared with the control group. *P < 0.05, **P < 0.01 compared with the PQ group.
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Figure 5: Effects of SF and OMT combination on the levels of CRP (a), IL-6 (b), NF-κB (c) in the lung homogenate of the PQ-intoxication rats. CON, PQ, SF and OMT represent the control group, paraquat group, sodium ferulate group (3.1 mg/Kg/day) and oxymatrine group (6.9 mg/Kg/day), respectively. SF+OMT represents sodium ferulate and oxymatrine combination treatment groups, and the 1.6 + 3.4, 3.1 + 6.9 and 6.2 + 13.8 respectively represent drug administration doses of SF + OMT in the low dose group, middle dose group and high dose group. Data are expressed as means ± SEM, n = 10 in each group. ##P < 0.01 compared with the control group. *P < 0.05, **P < 0.01 compared with the PQ group.

Mentions: As shown in Figure 5, after PQ injection, the lung homogenate CRP, IL-6 and NF-κB levels significantly increased in PQ group compared with the control. Compared with the PQ group, the levels of CRP, IL-6 and NF-κB significantly decreased in the middle dose group and high dose group of SF and OMT combination treatment. In the low dose group of combination treatment (SF+OMT 1.6 + 3.4 mg/Kg/day), only the level of IL-6 in lung homogenate decreased significantly (P < 0.05). Compared with PQ group, treatment with SF (3.1 mg/Kg/day) or OMT (6.9 mg/Kg/day) alone could not significantly decrease the levels of CRP, IL-6 and NF-κB in lung homogenate.


The Protective Effect of Sodium Ferulate and Oxymatrine Combination on Paraquat-induced Lung Injury.

Wang W, Pei X, Xu M, Sun S, Zhang C, Mu K, Liu Z - Iran J Pharm Res (2015)

Effects of SF and OMT combination on the levels of CRP (a), IL-6 (b), NF-κB (c) in the lung homogenate of the PQ-intoxication rats. CON, PQ, SF and OMT represent the control group, paraquat group, sodium ferulate group (3.1 mg/Kg/day) and oxymatrine group (6.9 mg/Kg/day), respectively. SF+OMT represents sodium ferulate and oxymatrine combination treatment groups, and the 1.6 + 3.4, 3.1 + 6.9 and 6.2 + 13.8 respectively represent drug administration doses of SF + OMT in the low dose group, middle dose group and high dose group. Data are expressed as means ± SEM, n = 10 in each group. ##P < 0.01 compared with the control group. *P < 0.05, **P < 0.01 compared with the PQ group.
© Copyright Policy
Related In: Results  -  Collection

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Show All Figures
getmorefigures.php?uid=PMC4403075&req=5

Figure 5: Effects of SF and OMT combination on the levels of CRP (a), IL-6 (b), NF-κB (c) in the lung homogenate of the PQ-intoxication rats. CON, PQ, SF and OMT represent the control group, paraquat group, sodium ferulate group (3.1 mg/Kg/day) and oxymatrine group (6.9 mg/Kg/day), respectively. SF+OMT represents sodium ferulate and oxymatrine combination treatment groups, and the 1.6 + 3.4, 3.1 + 6.9 and 6.2 + 13.8 respectively represent drug administration doses of SF + OMT in the low dose group, middle dose group and high dose group. Data are expressed as means ± SEM, n = 10 in each group. ##P < 0.01 compared with the control group. *P < 0.05, **P < 0.01 compared with the PQ group.
Mentions: As shown in Figure 5, after PQ injection, the lung homogenate CRP, IL-6 and NF-κB levels significantly increased in PQ group compared with the control. Compared with the PQ group, the levels of CRP, IL-6 and NF-κB significantly decreased in the middle dose group and high dose group of SF and OMT combination treatment. In the low dose group of combination treatment (SF+OMT 1.6 + 3.4 mg/Kg/day), only the level of IL-6 in lung homogenate decreased significantly (P < 0.05). Compared with PQ group, treatment with SF (3.1 mg/Kg/day) or OMT (6.9 mg/Kg/day) alone could not significantly decrease the levels of CRP, IL-6 and NF-κB in lung homogenate.

Bottom Line: Also in SF and OMT combination treatment groups of rats, the increased lung W/D ratio and histopathological score induced by PQ injection were significantly decreased; the levels of CRP, IL-6, NF-κB and MDA in serum and lung homogenate were significantly decreased; the SOD activities in serum and lung homogenate were improved.These results suggested that SF and OMT combination had an obvious protective effect on PQ-induced lung injury.The anti-inflammatory and antioxidant effect might be involved in the mechanism.

View Article: PubMed Central - PubMed

Affiliation: School of Pharmacy, Yantai University, No. 30, Qingquan Road, Laishan District, Yantai, Shandong, China.

ABSTRACT
Experimental evidence suggested that sodium ferulate (SF) and oxymatrine (OMT) combination had synergistic anti-inflammatory and antioxidant effects. We hypothesized that SF and OMT combination treatment might have protective effects on paraquat-induced acute lung injury. In our study, the Swiss mice were randomly divided into seven groups, including control, paraquat (PQ), SF (6.2 mg/Kg/day); OMT (13.8 mg/Kg/day) and three SF+OMT groups (3.1 + 6.9; 6.2 + 13.8 and 12.3 + 27.7 mg/Kg/day). The mortality and death time were monitored. Sprague-Dawley rats were randomly divided into seven groups including control, PQ, SF (3.1 mg/Kg/day); OMT (6.9 mg/Kg/day) and three SF+OMT groups (1.6 + 3.4; 3.1 + 6.9 and 6.2 + 13.8 mg/Kg/day). The lung wet/dry weight (W/D) ratio, lung histopathologic changes, C-reactive protein (CRP), interleukin-6 (IL-6), nuclear factor κB (NF-κB), malondialdehyde (MDA) and superoxidase dismutase (SOD) were analysed. Compared with PQ group, the mortality significantly decreased and the death time prolonged in SF and OMT combination treatment groups of mice. Also in SF and OMT combination treatment groups of rats, the increased lung W/D ratio and histopathological score induced by PQ injection were significantly decreased; the levels of CRP, IL-6, NF-κB and MDA in serum and lung homogenate were significantly decreased; the SOD activities in serum and lung homogenate were improved. These results suggested that SF and OMT combination had an obvious protective effect on PQ-induced lung injury. The anti-inflammatory and antioxidant effect might be involved in the mechanism.

No MeSH data available.


Related in: MedlinePlus