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The Effects of Nigella Sativa Hydro-alcoholic Extract on Memory and Brain Tissues Oxidative Damage after Repeated Seizures in Rats.

Vafaee F, Hosseini M, Hassanzadeh Z, Edalatmanesh MA, Sadeghnia HR, Seghatoleslam M, Mousavi SM, Amani A, Shafei MN - Iran J Pharm Res (2015)

Bottom Line: The total thiol concentration in hippocampal and cortical tissues of PTZ group was reduced while, MDA concentration was higher than control (p<0.05 - p<0.001).Administration of the extract increased the total thiol and decreased the MDA concentrations (p<0.01- p<0.001).It is concluded that the hydro-alcoholic extract of NS possess beneficial effects on learning and memory impairments in repeated seizures model which is accompanied by antioxidant effects in the brain.

View Article: PubMed Central - PubMed

Affiliation: Neurogenic Inflammation Research Center, Department of Physiology, School of Medicine, Mashhad, University of Medical Sciences, Mashhad, Iran.

ABSTRACT
Regarding the therapeutic properties of Nigella sativa (NS), the effects of the plant hydro - alcoholic extract on learning, memory and brain tissues oxidative damage were investigated in penthylenetetrazole (PTZ) - induced repeated seizures. There were 4 experimental groups including: 1- control group; received saline, 2- PTZ group ; received saline and PTZ (50 mg/Kg, i.p) , 3-PTZ- NS 200 and 4- PTZ- NS 400 ; received 200 and 400 mg/Kg of NS extract respectively, before PTZ injection in 5 consecutive days. Seizure scores were lower in PTZ - NS 200 and 400, furthermore the seizure onset latencies were higher in these groups than PTZ group (P<0.05 and P<0.01 ). In Morris water maze, the time spent in target quadrant by PTZ group was lower than control group (P<0.05); while, 400 mg/Kg of the extract increased it (P<0.01). In the passive avoidance test, delay time to enter the dark by PTZ group was lower than control at 1 and 24 hours after training (P<0.01- P<0.001); while, 400 mg/Kg of the extract increased it (P<0.05). The total thiol concentration in hippocampal and cortical tissues of PTZ group was reduced while, MDA concentration was higher than control (p<0.05 - p<0.001). Administration of the extract increased the total thiol and decreased the MDA concentrations (p<0.01- p<0.001). It is concluded that the hydro-alcoholic extract of NS possess beneficial effects on learning and memory impairments in repeated seizures model which is accompanied by antioxidant effects in the brain.

No MeSH data available.


Related in: MedlinePlus

The total thiol concentration (A) and MDA concentration (B) in hippocampal tissues of 4 groups. Data are shown as mean ± SEM of 9-10 animals per group.
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Figure 4: The total thiol concentration (A) and MDA concentration (B) in hippocampal tissues of 4 groups. Data are shown as mean ± SEM of 9-10 animals per group.

Mentions: The time latency for entering the dark compartment was lower in the PTZ group than control group 1 h after taking a shock (Figure 3A, P < 0.01). Treatment of the animals by 400 mg/Kg of NS extract significantly increased the time latency for entering the dark compartment 1 h after receiving a shock (Figure 3A, P < 0.05). However, there was no significant difference between PTZ-NS 200 and PTZ groups (Figure 3A). The time latency for entering the dark compartment in PTZ treated rats was lower than control group 24 h after the shock (Figure 4A, P < 0.001). Administration of 400 mg/Kg of the NS extract increased non-significantly the time latency for entering the dark compartment 24 hours after shock (Figure 3A). The time latency for entering the dark compartment didn’t change by 200 mg/Kg of the extract 24 h after taking a shock (Figure 3A).


The Effects of Nigella Sativa Hydro-alcoholic Extract on Memory and Brain Tissues Oxidative Damage after Repeated Seizures in Rats.

Vafaee F, Hosseini M, Hassanzadeh Z, Edalatmanesh MA, Sadeghnia HR, Seghatoleslam M, Mousavi SM, Amani A, Shafei MN - Iran J Pharm Res (2015)

The total thiol concentration (A) and MDA concentration (B) in hippocampal tissues of 4 groups. Data are shown as mean ± SEM of 9-10 animals per group.
© Copyright Policy
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4403072&req=5

Figure 4: The total thiol concentration (A) and MDA concentration (B) in hippocampal tissues of 4 groups. Data are shown as mean ± SEM of 9-10 animals per group.
Mentions: The time latency for entering the dark compartment was lower in the PTZ group than control group 1 h after taking a shock (Figure 3A, P < 0.01). Treatment of the animals by 400 mg/Kg of NS extract significantly increased the time latency for entering the dark compartment 1 h after receiving a shock (Figure 3A, P < 0.05). However, there was no significant difference between PTZ-NS 200 and PTZ groups (Figure 3A). The time latency for entering the dark compartment in PTZ treated rats was lower than control group 24 h after the shock (Figure 4A, P < 0.001). Administration of 400 mg/Kg of the NS extract increased non-significantly the time latency for entering the dark compartment 24 hours after shock (Figure 3A). The time latency for entering the dark compartment didn’t change by 200 mg/Kg of the extract 24 h after taking a shock (Figure 3A).

Bottom Line: The total thiol concentration in hippocampal and cortical tissues of PTZ group was reduced while, MDA concentration was higher than control (p<0.05 - p<0.001).Administration of the extract increased the total thiol and decreased the MDA concentrations (p<0.01- p<0.001).It is concluded that the hydro-alcoholic extract of NS possess beneficial effects on learning and memory impairments in repeated seizures model which is accompanied by antioxidant effects in the brain.

View Article: PubMed Central - PubMed

Affiliation: Neurogenic Inflammation Research Center, Department of Physiology, School of Medicine, Mashhad, University of Medical Sciences, Mashhad, Iran.

ABSTRACT
Regarding the therapeutic properties of Nigella sativa (NS), the effects of the plant hydro - alcoholic extract on learning, memory and brain tissues oxidative damage were investigated in penthylenetetrazole (PTZ) - induced repeated seizures. There were 4 experimental groups including: 1- control group; received saline, 2- PTZ group ; received saline and PTZ (50 mg/Kg, i.p) , 3-PTZ- NS 200 and 4- PTZ- NS 400 ; received 200 and 400 mg/Kg of NS extract respectively, before PTZ injection in 5 consecutive days. Seizure scores were lower in PTZ - NS 200 and 400, furthermore the seizure onset latencies were higher in these groups than PTZ group (P<0.05 and P<0.01 ). In Morris water maze, the time spent in target quadrant by PTZ group was lower than control group (P<0.05); while, 400 mg/Kg of the extract increased it (P<0.01). In the passive avoidance test, delay time to enter the dark by PTZ group was lower than control at 1 and 24 hours after training (P<0.01- P<0.001); while, 400 mg/Kg of the extract increased it (P<0.05). The total thiol concentration in hippocampal and cortical tissues of PTZ group was reduced while, MDA concentration was higher than control (p<0.05 - p<0.001). Administration of the extract increased the total thiol and decreased the MDA concentrations (p<0.01- p<0.001). It is concluded that the hydro-alcoholic extract of NS possess beneficial effects on learning and memory impairments in repeated seizures model which is accompanied by antioxidant effects in the brain.

No MeSH data available.


Related in: MedlinePlus