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The Effects of Nigella Sativa Hydro-alcoholic Extract on Memory and Brain Tissues Oxidative Damage after Repeated Seizures in Rats.

Vafaee F, Hosseini M, Hassanzadeh Z, Edalatmanesh MA, Sadeghnia HR, Seghatoleslam M, Mousavi SM, Amani A, Shafei MN - Iran J Pharm Res (2015)

Bottom Line: The total thiol concentration in hippocampal and cortical tissues of PTZ group was reduced while, MDA concentration was higher than control (p<0.05 - p<0.001).Administration of the extract increased the total thiol and decreased the MDA concentrations (p<0.01- p<0.001).It is concluded that the hydro-alcoholic extract of NS possess beneficial effects on learning and memory impairments in repeated seizures model which is accompanied by antioxidant effects in the brain.

View Article: PubMed Central - PubMed

Affiliation: Neurogenic Inflammation Research Center, Department of Physiology, School of Medicine, Mashhad, University of Medical Sciences, Mashhad, Iran.

ABSTRACT
Regarding the therapeutic properties of Nigella sativa (NS), the effects of the plant hydro - alcoholic extract on learning, memory and brain tissues oxidative damage were investigated in penthylenetetrazole (PTZ) - induced repeated seizures. There were 4 experimental groups including: 1- control group; received saline, 2- PTZ group ; received saline and PTZ (50 mg/Kg, i.p) , 3-PTZ- NS 200 and 4- PTZ- NS 400 ; received 200 and 400 mg/Kg of NS extract respectively, before PTZ injection in 5 consecutive days. Seizure scores were lower in PTZ - NS 200 and 400, furthermore the seizure onset latencies were higher in these groups than PTZ group (P<0.05 and P<0.01 ). In Morris water maze, the time spent in target quadrant by PTZ group was lower than control group (P<0.05); while, 400 mg/Kg of the extract increased it (P<0.01). In the passive avoidance test, delay time to enter the dark by PTZ group was lower than control at 1 and 24 hours after training (P<0.01- P<0.001); while, 400 mg/Kg of the extract increased it (P<0.05). The total thiol concentration in hippocampal and cortical tissues of PTZ group was reduced while, MDA concentration was higher than control (p<0.05 - p<0.001). Administration of the extract increased the total thiol and decreased the MDA concentrations (p<0.01- p<0.001). It is concluded that the hydro-alcoholic extract of NS possess beneficial effects on learning and memory impairments in repeated seizures model which is accompanied by antioxidant effects in the brain.

No MeSH data available.


Related in: MedlinePlus

Comparison of seizure score (A) and latency to the onset of seizures (B) between groups. Data are presented as mean ± SEM (n = 9-10 in each group). *p<0.05 and **p<0.01 compared to PTZ group. The animals were injected by PTZ and observed for 60 minutes. The animals of PTZ- NS 200 and PTZ- NS 400 groups were treated by 200 and 400 mg/Kg of Nigella sativa (NS) extract before PTZ injection. The animals of PTZ group received saline instead of NS extract.
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Figure 1: Comparison of seizure score (A) and latency to the onset of seizures (B) between groups. Data are presented as mean ± SEM (n = 9-10 in each group). *p<0.05 and **p<0.01 compared to PTZ group. The animals were injected by PTZ and observed for 60 minutes. The animals of PTZ- NS 200 and PTZ- NS 400 groups were treated by 200 and 400 mg/Kg of Nigella sativa (NS) extract before PTZ injection. The animals of PTZ group received saline instead of NS extract.

Mentions: The seizure scores in both PTZ - NS 200 and PTZ- NS 400 groups were significantly lower than PTZ group on days 3 and 4 (P<0.01 and P<0.05 respectively; Figure 1 A). The latency to the onset of seizures was also significantly higher than PTZ group on days 4 and 5 (P<0.05 and; P<0.01 Figure 1 B).


The Effects of Nigella Sativa Hydro-alcoholic Extract on Memory and Brain Tissues Oxidative Damage after Repeated Seizures in Rats.

Vafaee F, Hosseini M, Hassanzadeh Z, Edalatmanesh MA, Sadeghnia HR, Seghatoleslam M, Mousavi SM, Amani A, Shafei MN - Iran J Pharm Res (2015)

Comparison of seizure score (A) and latency to the onset of seizures (B) between groups. Data are presented as mean ± SEM (n = 9-10 in each group). *p<0.05 and **p<0.01 compared to PTZ group. The animals were injected by PTZ and observed for 60 minutes. The animals of PTZ- NS 200 and PTZ- NS 400 groups were treated by 200 and 400 mg/Kg of Nigella sativa (NS) extract before PTZ injection. The animals of PTZ group received saline instead of NS extract.
© Copyright Policy
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4403072&req=5

Figure 1: Comparison of seizure score (A) and latency to the onset of seizures (B) between groups. Data are presented as mean ± SEM (n = 9-10 in each group). *p<0.05 and **p<0.01 compared to PTZ group. The animals were injected by PTZ and observed for 60 minutes. The animals of PTZ- NS 200 and PTZ- NS 400 groups were treated by 200 and 400 mg/Kg of Nigella sativa (NS) extract before PTZ injection. The animals of PTZ group received saline instead of NS extract.
Mentions: The seizure scores in both PTZ - NS 200 and PTZ- NS 400 groups were significantly lower than PTZ group on days 3 and 4 (P<0.01 and P<0.05 respectively; Figure 1 A). The latency to the onset of seizures was also significantly higher than PTZ group on days 4 and 5 (P<0.05 and; P<0.01 Figure 1 B).

Bottom Line: The total thiol concentration in hippocampal and cortical tissues of PTZ group was reduced while, MDA concentration was higher than control (p<0.05 - p<0.001).Administration of the extract increased the total thiol and decreased the MDA concentrations (p<0.01- p<0.001).It is concluded that the hydro-alcoholic extract of NS possess beneficial effects on learning and memory impairments in repeated seizures model which is accompanied by antioxidant effects in the brain.

View Article: PubMed Central - PubMed

Affiliation: Neurogenic Inflammation Research Center, Department of Physiology, School of Medicine, Mashhad, University of Medical Sciences, Mashhad, Iran.

ABSTRACT
Regarding the therapeutic properties of Nigella sativa (NS), the effects of the plant hydro - alcoholic extract on learning, memory and brain tissues oxidative damage were investigated in penthylenetetrazole (PTZ) - induced repeated seizures. There were 4 experimental groups including: 1- control group; received saline, 2- PTZ group ; received saline and PTZ (50 mg/Kg, i.p) , 3-PTZ- NS 200 and 4- PTZ- NS 400 ; received 200 and 400 mg/Kg of NS extract respectively, before PTZ injection in 5 consecutive days. Seizure scores were lower in PTZ - NS 200 and 400, furthermore the seizure onset latencies were higher in these groups than PTZ group (P<0.05 and P<0.01 ). In Morris water maze, the time spent in target quadrant by PTZ group was lower than control group (P<0.05); while, 400 mg/Kg of the extract increased it (P<0.01). In the passive avoidance test, delay time to enter the dark by PTZ group was lower than control at 1 and 24 hours after training (P<0.01- P<0.001); while, 400 mg/Kg of the extract increased it (P<0.05). The total thiol concentration in hippocampal and cortical tissues of PTZ group was reduced while, MDA concentration was higher than control (p<0.05 - p<0.001). Administration of the extract increased the total thiol and decreased the MDA concentrations (p<0.01- p<0.001). It is concluded that the hydro-alcoholic extract of NS possess beneficial effects on learning and memory impairments in repeated seizures model which is accompanied by antioxidant effects in the brain.

No MeSH data available.


Related in: MedlinePlus