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The comparison of anticancer activity of thymoquinone and nanothymoquinone on human breast adenocarcinoma.

Dehghani H, Hashemi M, Entezari M, Mohsenifar A - Iran J Pharm Res (2015)

Bottom Line: There were significant differences in IC50 Thymoquinone and nanothymoquinone.TQ-loaded nanoparticles proved more effective compared to TQ solution.The high drug-targeting potential and efficiency demonstrates the significant role of the anticancer properties of TQ-loaded nanoparticles.

View Article: PubMed Central - PubMed

Affiliation: Department of Medical Laboratory Sciences, Islamic Azad University, Tehran Medical Sciences Branch, Tehran, Iran .

ABSTRACT
Cancer is one of the main causes of mortality in the world which is created by the effect of enviromental physico-chemical mutagen and carcinogen agents. The identification of new cytotoxic drugs with low side effects on immune system has developed as important area in new studies of pharmacology. Thymoquinone (TQ), derived from the medicinal spice Nigella sativa (also calledt black cumin) exhibit anti-inflammatory and anti-cancer activities. In this study we employed nanogel-based nanoparticle approach to improve upon its effectiveness. Myristic acid-chitosan (MA-chitosan) nanogels were prepared by the technique of self-assembly. Thymoquinone was loaded into the nanogels. The surface morphology of the prepared nanoparticles was determined using SEM and TEM. The other objective of this study was to examine the in-vitro cytotoxic activity of cell death of Thymoquinone and nanothymoquinone on human breast adenocarcinoma cell line (MCF7). Cytotoxicity and viability of Thymoquinone and nanothymoquinone were assessed by 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) and dye exclusion assay. Transmission electron microscopy confirmed the particle diameter was between 150 to 200 nm. Proliferation of MCF7 cells was significantly inhibited by Thymoquinone and nanothymoquinone in a concentration-dependent manner in defined times. There were significant differences in IC50 Thymoquinone and nanothymoquinone. TQ-loaded nanoparticles proved more effective compared to TQ solution. The high drug-targeting potential and efficiency demonstrates the significant role of the anticancer properties of TQ-loaded nanoparticles.

No MeSH data available.


Related in: MedlinePlus

The IC50 of Thymoquinone and nanothymoquinone for MCF-7 cell line
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Figure 3: The IC50 of Thymoquinone and nanothymoquinone for MCF-7 cell line

Mentions: The 50% inhibition concentration (IC50) values of Thymoquinone and nanothymoquinone on MCF7 cells were determined (Figure 3). IC50 was determined by probit analysis using the Pharm PCS (Pharmacologic Calculation System) statistical package (Springer- Verlag, USA). There were significant differences in IC50 Thymoquinone and nanothymoquinone (P<0.05).


The comparison of anticancer activity of thymoquinone and nanothymoquinone on human breast adenocarcinoma.

Dehghani H, Hashemi M, Entezari M, Mohsenifar A - Iran J Pharm Res (2015)

The IC50 of Thymoquinone and nanothymoquinone for MCF-7 cell line
© Copyright Policy
Related In: Results  -  Collection

Show All Figures
getmorefigures.php?uid=PMC4403071&req=5

Figure 3: The IC50 of Thymoquinone and nanothymoquinone for MCF-7 cell line
Mentions: The 50% inhibition concentration (IC50) values of Thymoquinone and nanothymoquinone on MCF7 cells were determined (Figure 3). IC50 was determined by probit analysis using the Pharm PCS (Pharmacologic Calculation System) statistical package (Springer- Verlag, USA). There were significant differences in IC50 Thymoquinone and nanothymoquinone (P<0.05).

Bottom Line: There were significant differences in IC50 Thymoquinone and nanothymoquinone.TQ-loaded nanoparticles proved more effective compared to TQ solution.The high drug-targeting potential and efficiency demonstrates the significant role of the anticancer properties of TQ-loaded nanoparticles.

View Article: PubMed Central - PubMed

Affiliation: Department of Medical Laboratory Sciences, Islamic Azad University, Tehran Medical Sciences Branch, Tehran, Iran .

ABSTRACT
Cancer is one of the main causes of mortality in the world which is created by the effect of enviromental physico-chemical mutagen and carcinogen agents. The identification of new cytotoxic drugs with low side effects on immune system has developed as important area in new studies of pharmacology. Thymoquinone (TQ), derived from the medicinal spice Nigella sativa (also calledt black cumin) exhibit anti-inflammatory and anti-cancer activities. In this study we employed nanogel-based nanoparticle approach to improve upon its effectiveness. Myristic acid-chitosan (MA-chitosan) nanogels were prepared by the technique of self-assembly. Thymoquinone was loaded into the nanogels. The surface morphology of the prepared nanoparticles was determined using SEM and TEM. The other objective of this study was to examine the in-vitro cytotoxic activity of cell death of Thymoquinone and nanothymoquinone on human breast adenocarcinoma cell line (MCF7). Cytotoxicity and viability of Thymoquinone and nanothymoquinone were assessed by 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) and dye exclusion assay. Transmission electron microscopy confirmed the particle diameter was between 150 to 200 nm. Proliferation of MCF7 cells was significantly inhibited by Thymoquinone and nanothymoquinone in a concentration-dependent manner in defined times. There were significant differences in IC50 Thymoquinone and nanothymoquinone. TQ-loaded nanoparticles proved more effective compared to TQ solution. The high drug-targeting potential and efficiency demonstrates the significant role of the anticancer properties of TQ-loaded nanoparticles.

No MeSH data available.


Related in: MedlinePlus