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The comparison of anticancer activity of thymoquinone and nanothymoquinone on human breast adenocarcinoma.

Dehghani H, Hashemi M, Entezari M, Mohsenifar A - Iran J Pharm Res (2015)

Bottom Line: There were significant differences in IC50 Thymoquinone and nanothymoquinone.TQ-loaded nanoparticles proved more effective compared to TQ solution.The high drug-targeting potential and efficiency demonstrates the significant role of the anticancer properties of TQ-loaded nanoparticles.

View Article: PubMed Central - PubMed

Affiliation: Department of Medical Laboratory Sciences, Islamic Azad University, Tehran Medical Sciences Branch, Tehran, Iran .

ABSTRACT
Cancer is one of the main causes of mortality in the world which is created by the effect of enviromental physico-chemical mutagen and carcinogen agents. The identification of new cytotoxic drugs with low side effects on immune system has developed as important area in new studies of pharmacology. Thymoquinone (TQ), derived from the medicinal spice Nigella sativa (also calledt black cumin) exhibit anti-inflammatory and anti-cancer activities. In this study we employed nanogel-based nanoparticle approach to improve upon its effectiveness. Myristic acid-chitosan (MA-chitosan) nanogels were prepared by the technique of self-assembly. Thymoquinone was loaded into the nanogels. The surface morphology of the prepared nanoparticles was determined using SEM and TEM. The other objective of this study was to examine the in-vitro cytotoxic activity of cell death of Thymoquinone and nanothymoquinone on human breast adenocarcinoma cell line (MCF7). Cytotoxicity and viability of Thymoquinone and nanothymoquinone were assessed by 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) and dye exclusion assay. Transmission electron microscopy confirmed the particle diameter was between 150 to 200 nm. Proliferation of MCF7 cells was significantly inhibited by Thymoquinone and nanothymoquinone in a concentration-dependent manner in defined times. There were significant differences in IC50 Thymoquinone and nanothymoquinone. TQ-loaded nanoparticles proved more effective compared to TQ solution. The high drug-targeting potential and efficiency demonstrates the significant role of the anticancer properties of TQ-loaded nanoparticles.

No MeSH data available.


Related in: MedlinePlus

Transmission electron (A) and scanning electron (B) microscopy study of optimized nanoparticles
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Figure 1: Transmission electron (A) and scanning electron (B) microscopy study of optimized nanoparticles

Mentions: Scanning and Transmission Electron Microscopy (SEM and TEM) was preformed to observe TLNs morphology, shape, size and core and shell structures. For preparing SEM photographs, one drop of TLN was dropped onto studs and gold sputtered using an auto sputter coater (BIORAD Polaron Division), then analyzed with Hitachi Field Emission Scanning Electron Microscope (FE-SEM) S-4160 (Japan) at 15 kV acceleration voltage. TEM was performed with the method of Liu et al. (13). By using Transmission Electron Microscope (Zeiss EM 10C TEM-GmbH) at an accelerating voltage of 80 Kv. The increasing amount of the Thymoquinone led to an increase in the particles sizes with irregular shape (Figure 1).


The comparison of anticancer activity of thymoquinone and nanothymoquinone on human breast adenocarcinoma.

Dehghani H, Hashemi M, Entezari M, Mohsenifar A - Iran J Pharm Res (2015)

Transmission electron (A) and scanning electron (B) microscopy study of optimized nanoparticles
© Copyright Policy
Related In: Results  -  Collection

Show All Figures
getmorefigures.php?uid=PMC4403071&req=5

Figure 1: Transmission electron (A) and scanning electron (B) microscopy study of optimized nanoparticles
Mentions: Scanning and Transmission Electron Microscopy (SEM and TEM) was preformed to observe TLNs morphology, shape, size and core and shell structures. For preparing SEM photographs, one drop of TLN was dropped onto studs and gold sputtered using an auto sputter coater (BIORAD Polaron Division), then analyzed with Hitachi Field Emission Scanning Electron Microscope (FE-SEM) S-4160 (Japan) at 15 kV acceleration voltage. TEM was performed with the method of Liu et al. (13). By using Transmission Electron Microscope (Zeiss EM 10C TEM-GmbH) at an accelerating voltage of 80 Kv. The increasing amount of the Thymoquinone led to an increase in the particles sizes with irregular shape (Figure 1).

Bottom Line: There were significant differences in IC50 Thymoquinone and nanothymoquinone.TQ-loaded nanoparticles proved more effective compared to TQ solution.The high drug-targeting potential and efficiency demonstrates the significant role of the anticancer properties of TQ-loaded nanoparticles.

View Article: PubMed Central - PubMed

Affiliation: Department of Medical Laboratory Sciences, Islamic Azad University, Tehran Medical Sciences Branch, Tehran, Iran .

ABSTRACT
Cancer is one of the main causes of mortality in the world which is created by the effect of enviromental physico-chemical mutagen and carcinogen agents. The identification of new cytotoxic drugs with low side effects on immune system has developed as important area in new studies of pharmacology. Thymoquinone (TQ), derived from the medicinal spice Nigella sativa (also calledt black cumin) exhibit anti-inflammatory and anti-cancer activities. In this study we employed nanogel-based nanoparticle approach to improve upon its effectiveness. Myristic acid-chitosan (MA-chitosan) nanogels were prepared by the technique of self-assembly. Thymoquinone was loaded into the nanogels. The surface morphology of the prepared nanoparticles was determined using SEM and TEM. The other objective of this study was to examine the in-vitro cytotoxic activity of cell death of Thymoquinone and nanothymoquinone on human breast adenocarcinoma cell line (MCF7). Cytotoxicity and viability of Thymoquinone and nanothymoquinone were assessed by 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) and dye exclusion assay. Transmission electron microscopy confirmed the particle diameter was between 150 to 200 nm. Proliferation of MCF7 cells was significantly inhibited by Thymoquinone and nanothymoquinone in a concentration-dependent manner in defined times. There were significant differences in IC50 Thymoquinone and nanothymoquinone. TQ-loaded nanoparticles proved more effective compared to TQ solution. The high drug-targeting potential and efficiency demonstrates the significant role of the anticancer properties of TQ-loaded nanoparticles.

No MeSH data available.


Related in: MedlinePlus