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Molecular mechanisms of luteolin induced growth inhibition and apoptosis of human osteosarcoma cells.

Wang Y, Kong D, Wang X, Dong X, Tao Y, Gong H - Iran J Pharm Res (2015)

Bottom Line: It is a natural anti-oxidant with less pro-oxidant potential but apparently with a better safety profile.Furthermore, the inhibitory effects of luteolin on the expressions of BCL-2, BAX, Caspase-3 and Survivin were detected by Western blotting.Western blotting demonstrated that luteolin (0.5, 2.5, 12.5 µg/mL) inhibited the expressions of BCL-2, Caspase-3 and Survivin, and promoted the expression of BAX in MG-63 cells with a concentration dependent way.

View Article: PubMed Central - PubMed

Affiliation: Department of Orthopedics, 86 Hospital of PLA, Dangtu, Anhui 243100, China.

ABSTRACT
Luteolin is a flavone in medicinal plants as well as some vegetables and spices. It is a natural anti-oxidant with less pro-oxidant potential but apparently with a better safety profile. The purpose of this study was to investigate the molecular mechanism of luteolin-mediated apoptosis of MG-63 human osteosarcoma cells. MTT assay kit was employed to evaluate the effects of luteolin on MG-63 cells proliferation. Then, we performed Annexin V-FITC/PI to analyze the apoptotic rate of the cells. Furthermore, the inhibitory effects of luteolin on the expressions of BCL-2, BAX, Caspase-3 and Survivin were detected by Western blotting. As expected, luteolin (0.5, 2.5, 12.5 µg/mL) inhibited the growth of MG-63 cells by inhibiting cell proliferation and inducing cell apoptosis. Western blotting demonstrated that luteolin (0.5, 2.5, 12.5 µg/mL) inhibited the expressions of BCL-2, Caspase-3 and Survivin, and promoted the expression of BAX in MG-63 cells with a concentration dependent way. Luteolin can inhibit osteosarcoma cell proliferation and induce apoptosis effectively in a dose dependent manner through down-regulating the expression of BCL-2, Caspase-3 and Survivin proteins levels and up-regulating the expression of BAX protein level. These findings indicated that luteolin may be used as a novel herbal medicine for the treatment of osteosarcoma.

No MeSH data available.


Related in: MedlinePlus

The protein expression levels of BCL-2, BAX and caspase-3 after the treatment of luteolin (0, 0.5, 2.5, 12.5 μg/mL, respectively) for 24 hours. 0: control group; 0.5: Luteolin (0.5 μg/mL); 2.5: Luteolin (2.5 μg/mL); 12.5: Luteolin (12.5 μg/mL)
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Figure 3: The protein expression levels of BCL-2, BAX and caspase-3 after the treatment of luteolin (0, 0.5, 2.5, 12.5 μg/mL, respectively) for 24 hours. 0: control group; 0.5: Luteolin (0.5 μg/mL); 2.5: Luteolin (2.5 μg/mL); 12.5: Luteolin (12.5 μg/mL)

Mentions: The protein expression levels of BCL-2, BAX and Caspase-3 were assessed by Western blotting. Compared with the control group, the protein expression levels of BCL-2 and Caspase-3 were significantly decreased, while the expression levels of BAX significantly increased in the luteolin group (Figure 3, P < 0.05). This result indicated that the BAX/BCL-2 ratio was also significantly increased. Therefore, luteolin might induce apoptosis of MG-63 cells through the mitochondrial pathway.


Molecular mechanisms of luteolin induced growth inhibition and apoptosis of human osteosarcoma cells.

Wang Y, Kong D, Wang X, Dong X, Tao Y, Gong H - Iran J Pharm Res (2015)

The protein expression levels of BCL-2, BAX and caspase-3 after the treatment of luteolin (0, 0.5, 2.5, 12.5 μg/mL, respectively) for 24 hours. 0: control group; 0.5: Luteolin (0.5 μg/mL); 2.5: Luteolin (2.5 μg/mL); 12.5: Luteolin (12.5 μg/mL)
© Copyright Policy
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4403070&req=5

Figure 3: The protein expression levels of BCL-2, BAX and caspase-3 after the treatment of luteolin (0, 0.5, 2.5, 12.5 μg/mL, respectively) for 24 hours. 0: control group; 0.5: Luteolin (0.5 μg/mL); 2.5: Luteolin (2.5 μg/mL); 12.5: Luteolin (12.5 μg/mL)
Mentions: The protein expression levels of BCL-2, BAX and Caspase-3 were assessed by Western blotting. Compared with the control group, the protein expression levels of BCL-2 and Caspase-3 were significantly decreased, while the expression levels of BAX significantly increased in the luteolin group (Figure 3, P < 0.05). This result indicated that the BAX/BCL-2 ratio was also significantly increased. Therefore, luteolin might induce apoptosis of MG-63 cells through the mitochondrial pathway.

Bottom Line: It is a natural anti-oxidant with less pro-oxidant potential but apparently with a better safety profile.Furthermore, the inhibitory effects of luteolin on the expressions of BCL-2, BAX, Caspase-3 and Survivin were detected by Western blotting.Western blotting demonstrated that luteolin (0.5, 2.5, 12.5 µg/mL) inhibited the expressions of BCL-2, Caspase-3 and Survivin, and promoted the expression of BAX in MG-63 cells with a concentration dependent way.

View Article: PubMed Central - PubMed

Affiliation: Department of Orthopedics, 86 Hospital of PLA, Dangtu, Anhui 243100, China.

ABSTRACT
Luteolin is a flavone in medicinal plants as well as some vegetables and spices. It is a natural anti-oxidant with less pro-oxidant potential but apparently with a better safety profile. The purpose of this study was to investigate the molecular mechanism of luteolin-mediated apoptosis of MG-63 human osteosarcoma cells. MTT assay kit was employed to evaluate the effects of luteolin on MG-63 cells proliferation. Then, we performed Annexin V-FITC/PI to analyze the apoptotic rate of the cells. Furthermore, the inhibitory effects of luteolin on the expressions of BCL-2, BAX, Caspase-3 and Survivin were detected by Western blotting. As expected, luteolin (0.5, 2.5, 12.5 µg/mL) inhibited the growth of MG-63 cells by inhibiting cell proliferation and inducing cell apoptosis. Western blotting demonstrated that luteolin (0.5, 2.5, 12.5 µg/mL) inhibited the expressions of BCL-2, Caspase-3 and Survivin, and promoted the expression of BAX in MG-63 cells with a concentration dependent way. Luteolin can inhibit osteosarcoma cell proliferation and induce apoptosis effectively in a dose dependent manner through down-regulating the expression of BCL-2, Caspase-3 and Survivin proteins levels and up-regulating the expression of BAX protein level. These findings indicated that luteolin may be used as a novel herbal medicine for the treatment of osteosarcoma.

No MeSH data available.


Related in: MedlinePlus