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Comparing the Effect of Silybin and Silybin Advanced™ on Viability and HER2 Expression on the Human Breast Cancer SKBR3 Cell Line by no Serum Starvation.

Mahmoodi N, Motamed N, Paylakhi SH, O Mahmoodi N - Iran J Pharm Res (2015)

Bottom Line: The poor bioavailability of some polyphenols (flavonoids, and terpenoids) can be improved by binding them to phosphatidylcholine (phytosome technology).Many studies have focused on the most common phytosome, silybin-phosphatidylcholine, particularly for its hepatoprotective effects.However, in recent years, studies have also been conducted to determine its anti-cancer effect.

View Article: PubMed Central - PubMed

Affiliation: Department of Cell and Molecular Biology, Kish International Campus, University of Tehran, Kish, Iran. ; School of Biology, University College of Science, University of Tehran, Tehran, Iran.

ABSTRACT
The polyphenol silybin has anti-oxidant and anti-cancer properties. The poor bioavailability of some polyphenols (flavonoids, and terpenoids) can be improved by binding them to phosphatidylcholine (phytosome technology). Many studies have focused on the most common phytosome, silybin-phosphatidylcholine, particularly for its hepatoprotective effects. However, in recent years, studies have also been conducted to determine its anti-cancer effect. Considering that the serum starvation should not be used for studies that are not focused on cell cycle arrest, we studied the effect of silybin-phosphatidylcholine from Silybin Advanced™ in 1:2 ratio (one part silybin bound to two parts phosphatidylcholine) on HER2 gene expression on the SKBR3 breast cancer cell line which were cultured in complete medium (not serum deprivation). The results were compared with our previous study of silybin on HER2 expression on SKBR3 cells. An MTT test was used to determine concentrations for cell treatment, and the gene expression was defined by real-time RT-PCR. Outcomes showed significant concentration- and time-dependent cell growth inhibitory effects of silybin, and silybin-phosphatidylcholine and HER2 down regulation on SKBR3 cells. Silybin-phosphatidylcholine concentrations had a much larger inhibitory and HER2 down regulate effect on cell growth than the same silybin concentrations on SKBR3 cells.

No MeSH data available.


Related in: MedlinePlus

Effect of Silybin, and Silybin-phosphatidylcholine on HER-2 mRNA expression after 24 (A), 48 (B), and 72 h (C) on the SKBR3 breast cancer cell line by real-time RT-PCR. Relative expression graphs were depicted by SPSS 18 (simple bar, summaries for group of case). Data were presented as two independent experiments. Each experiment had two individual samples (Error bars: +/- 1 SD). The p-values were estimated by SPSS 18, One-way ANOVA, and Dunnett-t two-sided post hoc tests.
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Figure 4: Effect of Silybin, and Silybin-phosphatidylcholine on HER-2 mRNA expression after 24 (A), 48 (B), and 72 h (C) on the SKBR3 breast cancer cell line by real-time RT-PCR. Relative expression graphs were depicted by SPSS 18 (simple bar, summaries for group of case). Data were presented as two independent experiments. Each experiment had two individual samples (Error bars: +/- 1 SD). The p-values were estimated by SPSS 18, One-way ANOVA, and Dunnett-t two-sided post hoc tests.

Mentions: Data were analyzed using SPSS 18 software. One-way ANOVA, and Dunnett-t two-sidedpost hoc tests were employed to evaluate the statistical significance of differences between the control, and all treatments. The p-values that were considered significant are displayed *: P < 0.05, **: P < 0.01, ***: P < 0.001 in Figures 2 and 4).


Comparing the Effect of Silybin and Silybin Advanced™ on Viability and HER2 Expression on the Human Breast Cancer SKBR3 Cell Line by no Serum Starvation.

Mahmoodi N, Motamed N, Paylakhi SH, O Mahmoodi N - Iran J Pharm Res (2015)

Effect of Silybin, and Silybin-phosphatidylcholine on HER-2 mRNA expression after 24 (A), 48 (B), and 72 h (C) on the SKBR3 breast cancer cell line by real-time RT-PCR. Relative expression graphs were depicted by SPSS 18 (simple bar, summaries for group of case). Data were presented as two independent experiments. Each experiment had two individual samples (Error bars: +/- 1 SD). The p-values were estimated by SPSS 18, One-way ANOVA, and Dunnett-t two-sided post hoc tests.
© Copyright Policy
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4403069&req=5

Figure 4: Effect of Silybin, and Silybin-phosphatidylcholine on HER-2 mRNA expression after 24 (A), 48 (B), and 72 h (C) on the SKBR3 breast cancer cell line by real-time RT-PCR. Relative expression graphs were depicted by SPSS 18 (simple bar, summaries for group of case). Data were presented as two independent experiments. Each experiment had two individual samples (Error bars: +/- 1 SD). The p-values were estimated by SPSS 18, One-way ANOVA, and Dunnett-t two-sided post hoc tests.
Mentions: Data were analyzed using SPSS 18 software. One-way ANOVA, and Dunnett-t two-sidedpost hoc tests were employed to evaluate the statistical significance of differences between the control, and all treatments. The p-values that were considered significant are displayed *: P < 0.05, **: P < 0.01, ***: P < 0.001 in Figures 2 and 4).

Bottom Line: The poor bioavailability of some polyphenols (flavonoids, and terpenoids) can be improved by binding them to phosphatidylcholine (phytosome technology).Many studies have focused on the most common phytosome, silybin-phosphatidylcholine, particularly for its hepatoprotective effects.However, in recent years, studies have also been conducted to determine its anti-cancer effect.

View Article: PubMed Central - PubMed

Affiliation: Department of Cell and Molecular Biology, Kish International Campus, University of Tehran, Kish, Iran. ; School of Biology, University College of Science, University of Tehran, Tehran, Iran.

ABSTRACT
The polyphenol silybin has anti-oxidant and anti-cancer properties. The poor bioavailability of some polyphenols (flavonoids, and terpenoids) can be improved by binding them to phosphatidylcholine (phytosome technology). Many studies have focused on the most common phytosome, silybin-phosphatidylcholine, particularly for its hepatoprotective effects. However, in recent years, studies have also been conducted to determine its anti-cancer effect. Considering that the serum starvation should not be used for studies that are not focused on cell cycle arrest, we studied the effect of silybin-phosphatidylcholine from Silybin Advanced™ in 1:2 ratio (one part silybin bound to two parts phosphatidylcholine) on HER2 gene expression on the SKBR3 breast cancer cell line which were cultured in complete medium (not serum deprivation). The results were compared with our previous study of silybin on HER2 expression on SKBR3 cells. An MTT test was used to determine concentrations for cell treatment, and the gene expression was defined by real-time RT-PCR. Outcomes showed significant concentration- and time-dependent cell growth inhibitory effects of silybin, and silybin-phosphatidylcholine and HER2 down regulation on SKBR3 cells. Silybin-phosphatidylcholine concentrations had a much larger inhibitory and HER2 down regulate effect on cell growth than the same silybin concentrations on SKBR3 cells.

No MeSH data available.


Related in: MedlinePlus