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Comparing the Effect of Silybin and Silybin Advanced™ on Viability and HER2 Expression on the Human Breast Cancer SKBR3 Cell Line by no Serum Starvation.

Mahmoodi N, Motamed N, Paylakhi SH, O Mahmoodi N - Iran J Pharm Res (2015)

Bottom Line: The poor bioavailability of some polyphenols (flavonoids, and terpenoids) can be improved by binding them to phosphatidylcholine (phytosome technology).Many studies have focused on the most common phytosome, silybin-phosphatidylcholine, particularly for its hepatoprotective effects.However, in recent years, studies have also been conducted to determine its anti-cancer effect.

View Article: PubMed Central - PubMed

Affiliation: Department of Cell and Molecular Biology, Kish International Campus, University of Tehran, Kish, Iran. ; School of Biology, University College of Science, University of Tehran, Tehran, Iran.

ABSTRACT
The polyphenol silybin has anti-oxidant and anti-cancer properties. The poor bioavailability of some polyphenols (flavonoids, and terpenoids) can be improved by binding them to phosphatidylcholine (phytosome technology). Many studies have focused on the most common phytosome, silybin-phosphatidylcholine, particularly for its hepatoprotective effects. However, in recent years, studies have also been conducted to determine its anti-cancer effect. Considering that the serum starvation should not be used for studies that are not focused on cell cycle arrest, we studied the effect of silybin-phosphatidylcholine from Silybin Advanced™ in 1:2 ratio (one part silybin bound to two parts phosphatidylcholine) on HER2 gene expression on the SKBR3 breast cancer cell line which were cultured in complete medium (not serum deprivation). The results were compared with our previous study of silybin on HER2 expression on SKBR3 cells. An MTT test was used to determine concentrations for cell treatment, and the gene expression was defined by real-time RT-PCR. Outcomes showed significant concentration- and time-dependent cell growth inhibitory effects of silybin, and silybin-phosphatidylcholine and HER2 down regulation on SKBR3 cells. Silybin-phosphatidylcholine concentrations had a much larger inhibitory and HER2 down regulate effect on cell growth than the same silybin concentrations on SKBR3 cells.

No MeSH data available.


Related in: MedlinePlus

The chemical structure of A) silybin and B) silybin-phosphatidylcholine
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Figure 1: The chemical structure of A) silybin and B) silybin-phosphatidylcholine

Mentions: Recent studies have suggests that finding a component that regulates gene expression would be useful for all kinds of cancer treatments. Most anti-cancer drugs for the HER2 receptor are receptor blockers, and not gene regulators. Serum starvation commonly leads to cell cycle arrest in the G0/G1 phase, and also has been used to arrest the G1 phase in cancer cells (25). In our latest research, the comparison of silybin IC50s, using complete medium and serum starvation procedures on BT474 or MDA-MB-453 cell line indicates that the IC50s reported doses by serum starvation method are less than the complete medium method (data not shown). Hence, in this study, firstly we considered that potential of silybin (natural polyphenol) (Figure 1 A) and silybin-phosphatidylcholine (we extracted from capsules) (Figure 1B) as a gene regulator for HER2. Secondly we evaluated if silybin-phosphatidylcholine in 1:2 ratio (not 1:1) is more effective than silybin to down regulate HER2 gene expression on SKBR3 cell line in complete medium.


Comparing the Effect of Silybin and Silybin Advanced™ on Viability and HER2 Expression on the Human Breast Cancer SKBR3 Cell Line by no Serum Starvation.

Mahmoodi N, Motamed N, Paylakhi SH, O Mahmoodi N - Iran J Pharm Res (2015)

The chemical structure of A) silybin and B) silybin-phosphatidylcholine
© Copyright Policy
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4403069&req=5

Figure 1: The chemical structure of A) silybin and B) silybin-phosphatidylcholine
Mentions: Recent studies have suggests that finding a component that regulates gene expression would be useful for all kinds of cancer treatments. Most anti-cancer drugs for the HER2 receptor are receptor blockers, and not gene regulators. Serum starvation commonly leads to cell cycle arrest in the G0/G1 phase, and also has been used to arrest the G1 phase in cancer cells (25). In our latest research, the comparison of silybin IC50s, using complete medium and serum starvation procedures on BT474 or MDA-MB-453 cell line indicates that the IC50s reported doses by serum starvation method are less than the complete medium method (data not shown). Hence, in this study, firstly we considered that potential of silybin (natural polyphenol) (Figure 1 A) and silybin-phosphatidylcholine (we extracted from capsules) (Figure 1B) as a gene regulator for HER2. Secondly we evaluated if silybin-phosphatidylcholine in 1:2 ratio (not 1:1) is more effective than silybin to down regulate HER2 gene expression on SKBR3 cell line in complete medium.

Bottom Line: The poor bioavailability of some polyphenols (flavonoids, and terpenoids) can be improved by binding them to phosphatidylcholine (phytosome technology).Many studies have focused on the most common phytosome, silybin-phosphatidylcholine, particularly for its hepatoprotective effects.However, in recent years, studies have also been conducted to determine its anti-cancer effect.

View Article: PubMed Central - PubMed

Affiliation: Department of Cell and Molecular Biology, Kish International Campus, University of Tehran, Kish, Iran. ; School of Biology, University College of Science, University of Tehran, Tehran, Iran.

ABSTRACT
The polyphenol silybin has anti-oxidant and anti-cancer properties. The poor bioavailability of some polyphenols (flavonoids, and terpenoids) can be improved by binding them to phosphatidylcholine (phytosome technology). Many studies have focused on the most common phytosome, silybin-phosphatidylcholine, particularly for its hepatoprotective effects. However, in recent years, studies have also been conducted to determine its anti-cancer effect. Considering that the serum starvation should not be used for studies that are not focused on cell cycle arrest, we studied the effect of silybin-phosphatidylcholine from Silybin Advanced™ in 1:2 ratio (one part silybin bound to two parts phosphatidylcholine) on HER2 gene expression on the SKBR3 breast cancer cell line which were cultured in complete medium (not serum deprivation). The results were compared with our previous study of silybin on HER2 expression on SKBR3 cells. An MTT test was used to determine concentrations for cell treatment, and the gene expression was defined by real-time RT-PCR. Outcomes showed significant concentration- and time-dependent cell growth inhibitory effects of silybin, and silybin-phosphatidylcholine and HER2 down regulation on SKBR3 cells. Silybin-phosphatidylcholine concentrations had a much larger inhibitory and HER2 down regulate effect on cell growth than the same silybin concentrations on SKBR3 cells.

No MeSH data available.


Related in: MedlinePlus