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UV Spectrophotometric Determination and Validation of Hydroquinone in Liposome.

Khoshneviszadeh R, Fazly Bazzaz BS, Housaindokht MR, Ebrahim-Habibi A, Rajabi O - Iran J Pharm Res (2015)

Bottom Line: Furthermore, various validation parameters as per ICH Q2B guideline were tested and found accordingly.The recovery percentages of liposomal hydroquinone were found 102 ± 0.8, 99 ± 0.2 and 98 ± 0.4 for 80%, 100% and 120% respectively.LOD and LOQ were 0.24 and 0.72 µg/mL respectively.

View Article: PubMed Central - PubMed

Affiliation: Department of Biology, Science and Research Branch, Islamic Azad University, Tehran, Iran.

ABSTRACT
The method has been developed and validated for the determination of hydroquinone in liposomal formulation. The samples were dissolved in methanol and evaluated in 293 nm. The validation parameters such as linearity, accuracy, precision, specificity, limit of detection (LOD) and limit of quantitation (LOQ) were determined. The calibration curve was linear in 1-50 µg/mL range of hydroquinone analyte with a regression coefficient of 0.9998. This study showed that the liposomal hydroquinone composed of phospholipid (7.8 %), cholesterol (1.5 %), alpha ketopherol (0.17 %) and hydroquinone (0.5 %) did not absorb wavelength of 293 nm if it diluted 500 times by methanol. The concentration of hydroquinone reached 10 µg/mL after 500 times of dilution. Furthermore, various validation parameters as per ICH Q2B guideline were tested and found accordingly. The recovery percentages of liposomal hydroquinone were found 102 ± 0.8, 99 ± 0.2 and 98 ± 0.4 for 80%, 100% and 120% respectively. The relative standard deviation values of inter and intra-day precisions were <%2. LOD and LOQ were 0.24 and 0.72 µg/mL respectively.

No MeSH data available.


Related in: MedlinePlus

Hydroquinone standard solution (10 µg/mL) mixed with Hydroquinone-free liposome solution diluted 500 times (orange). Hydroquinone standard solution in 10 µg/mL concentration (blue)
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Figure 6: Hydroquinone standard solution (10 µg/mL) mixed with Hydroquinone-free liposome solution diluted 500 times (orange). Hydroquinone standard solution in 10 µg/mL concentration (blue)

Mentions: HQ-free Liposome Spectrum in different dilutions was outlined in Figure 4. When it was diluted 500 times, there was no absorption in 293 nm. The 2-spectrum overlay of HQ-free liposome solution with 500 times of dilution and HQ standard solution (10 µg/mL) didn’t show any interface in 293 nm (Figure 5). Also, absorption solution of HQ standard (10 µg/mL) mixed with HQ-free liposome solution diluted 500 times was the same HQ standard solution (Figure 6).


UV Spectrophotometric Determination and Validation of Hydroquinone in Liposome.

Khoshneviszadeh R, Fazly Bazzaz BS, Housaindokht MR, Ebrahim-Habibi A, Rajabi O - Iran J Pharm Res (2015)

Hydroquinone standard solution (10 µg/mL) mixed with Hydroquinone-free liposome solution diluted 500 times (orange). Hydroquinone standard solution in 10 µg/mL concentration (blue)
© Copyright Policy
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4403063&req=5

Figure 6: Hydroquinone standard solution (10 µg/mL) mixed with Hydroquinone-free liposome solution diluted 500 times (orange). Hydroquinone standard solution in 10 µg/mL concentration (blue)
Mentions: HQ-free Liposome Spectrum in different dilutions was outlined in Figure 4. When it was diluted 500 times, there was no absorption in 293 nm. The 2-spectrum overlay of HQ-free liposome solution with 500 times of dilution and HQ standard solution (10 µg/mL) didn’t show any interface in 293 nm (Figure 5). Also, absorption solution of HQ standard (10 µg/mL) mixed with HQ-free liposome solution diluted 500 times was the same HQ standard solution (Figure 6).

Bottom Line: Furthermore, various validation parameters as per ICH Q2B guideline were tested and found accordingly.The recovery percentages of liposomal hydroquinone were found 102 ± 0.8, 99 ± 0.2 and 98 ± 0.4 for 80%, 100% and 120% respectively.LOD and LOQ were 0.24 and 0.72 µg/mL respectively.

View Article: PubMed Central - PubMed

Affiliation: Department of Biology, Science and Research Branch, Islamic Azad University, Tehran, Iran.

ABSTRACT
The method has been developed and validated for the determination of hydroquinone in liposomal formulation. The samples were dissolved in methanol and evaluated in 293 nm. The validation parameters such as linearity, accuracy, precision, specificity, limit of detection (LOD) and limit of quantitation (LOQ) were determined. The calibration curve was linear in 1-50 µg/mL range of hydroquinone analyte with a regression coefficient of 0.9998. This study showed that the liposomal hydroquinone composed of phospholipid (7.8 %), cholesterol (1.5 %), alpha ketopherol (0.17 %) and hydroquinone (0.5 %) did not absorb wavelength of 293 nm if it diluted 500 times by methanol. The concentration of hydroquinone reached 10 µg/mL after 500 times of dilution. Furthermore, various validation parameters as per ICH Q2B guideline were tested and found accordingly. The recovery percentages of liposomal hydroquinone were found 102 ± 0.8, 99 ± 0.2 and 98 ± 0.4 for 80%, 100% and 120% respectively. The relative standard deviation values of inter and intra-day precisions were <%2. LOD and LOQ were 0.24 and 0.72 µg/mL respectively.

No MeSH data available.


Related in: MedlinePlus