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Amperometric Determination of Ascorbic Acid in Pharmaceutical Formulations by a Reduced Graphene Oxide-cobalt Hexacyanoferrate Nanocomposite.

Heli H - Iran J Pharm Res (2015)

Bottom Line: A nanocomposite of reduced graphene oxide-cobalt hexacyanoferrate was synthesized by a simple precipitation route.Scanning electron microscopy revealed that the nanocomposite comprised nanoparticles of cobalt hexacyanoferrate attached to the reduced graphene oxide nanosheets.Finally, an amperometric method was developed for the quantification of ascorbic acid in different pharmaceutical formulations.

View Article: PubMed Central - PubMed

Affiliation: Nanomedicine and Nanobiology Research Center, Shiraz University of Medical Sciences , Shiraz, Iran. ; Department of Nanomedicine, School of Advanced Medical Sciences and Technologies, Shiraz University of Medical Sciences, Shiraz, Iran.

ABSTRACT
Investigation of the redox properties of drugs and their determination are performed by electrochemical techniques. Data obtained from electrochemical techniques are often correlated with molecular structure and pharmacological activity of drugs. In this regard, different modified electrodes were applied as sensors for quantification of different drugs. A nanocomposite of reduced graphene oxide-cobalt hexacyanoferrate was synthesized by a simple precipitation route. Scanning electron microscopy revealed that the nanocomposite comprised nanoparticles of cobalt hexacyanoferrate attached to the reduced graphene oxide nanosheets. A nanocomposite-modified carbon paste electrode was then fabricated. It represented prominent activity toward the electrocatalytic oxidation of ascorbic acid, and the kinetics of the electrooxidation process was evaluated. Finally, an amperometric method was developed for the quantification of ascorbic acid in different pharmaceutical formulations.

No MeSH data available.


Typical amperometric signals obtained during successive increment in the AA concentration. Inset: The calibration curve.
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Figure 6: Typical amperometric signals obtained during successive increment in the AA concentration. Inset: The calibration curve.

Mentions: In order to develop an electrochemical sensor for the analysis of AA, an amperometric method was developed. Typical amperometric signals obtained during successive addition of AA to PBS are shown in Figure 6. Gentle stirring for a few seconds was needed to promote solution homogenization after each injection. The electrode response was quite rapid and proportional to the AA concentration. The corresponding calibration curve for the determination of AA is shown in Figure 6, inset. The limits of detection (LOD) and quantification (LOQ) of the procedure were obtained as 3SD/m and 10SD/m, respectively, where SD is the standard deviation of the intercept and m is the slope of the calibration curve )42(. The determined parameters for the calibration curves of AA are reported in Table 1.


Amperometric Determination of Ascorbic Acid in Pharmaceutical Formulations by a Reduced Graphene Oxide-cobalt Hexacyanoferrate Nanocomposite.

Heli H - Iran J Pharm Res (2015)

Typical amperometric signals obtained during successive increment in the AA concentration. Inset: The calibration curve.
© Copyright Policy
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4403061&req=5

Figure 6: Typical amperometric signals obtained during successive increment in the AA concentration. Inset: The calibration curve.
Mentions: In order to develop an electrochemical sensor for the analysis of AA, an amperometric method was developed. Typical amperometric signals obtained during successive addition of AA to PBS are shown in Figure 6. Gentle stirring for a few seconds was needed to promote solution homogenization after each injection. The electrode response was quite rapid and proportional to the AA concentration. The corresponding calibration curve for the determination of AA is shown in Figure 6, inset. The limits of detection (LOD) and quantification (LOQ) of the procedure were obtained as 3SD/m and 10SD/m, respectively, where SD is the standard deviation of the intercept and m is the slope of the calibration curve )42(. The determined parameters for the calibration curves of AA are reported in Table 1.

Bottom Line: A nanocomposite of reduced graphene oxide-cobalt hexacyanoferrate was synthesized by a simple precipitation route.Scanning electron microscopy revealed that the nanocomposite comprised nanoparticles of cobalt hexacyanoferrate attached to the reduced graphene oxide nanosheets.Finally, an amperometric method was developed for the quantification of ascorbic acid in different pharmaceutical formulations.

View Article: PubMed Central - PubMed

Affiliation: Nanomedicine and Nanobiology Research Center, Shiraz University of Medical Sciences , Shiraz, Iran. ; Department of Nanomedicine, School of Advanced Medical Sciences and Technologies, Shiraz University of Medical Sciences, Shiraz, Iran.

ABSTRACT
Investigation of the redox properties of drugs and their determination are performed by electrochemical techniques. Data obtained from electrochemical techniques are often correlated with molecular structure and pharmacological activity of drugs. In this regard, different modified electrodes were applied as sensors for quantification of different drugs. A nanocomposite of reduced graphene oxide-cobalt hexacyanoferrate was synthesized by a simple precipitation route. Scanning electron microscopy revealed that the nanocomposite comprised nanoparticles of cobalt hexacyanoferrate attached to the reduced graphene oxide nanosheets. A nanocomposite-modified carbon paste electrode was then fabricated. It represented prominent activity toward the electrocatalytic oxidation of ascorbic acid, and the kinetics of the electrooxidation process was evaluated. Finally, an amperometric method was developed for the quantification of ascorbic acid in different pharmaceutical formulations.

No MeSH data available.