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Determination of bosentan in pharmaceutical preparations by linear sweep, square wave and differential pulse voltammetry methods.

Atila A, Yilmaz B - Iran J Pharm Res (2015)

Bottom Line: Intra- and inter-day precision values for bosentan were less than 4.92, and accuracy (relative error) was better than 6.29%.The mean recovery of bosentan was 100.7% for pharmaceutical preparations.No interference was found from two tablet excipients at the selected assay conditions.

View Article: PubMed Central - PubMed

Affiliation: Department of Analytical Chemistry, Faculty of Pharmacy, Ataturk University, 25240, Erzurum, Turkey.

ABSTRACT
In this study, simple, fast and reliable cyclic voltammetry (CV), linear sweep voltammetry (LSV), square wave voltammetry (SWV) and differential pulse voltammetry (DPV) methods were developed and validated for determination of bosentan in pharmaceutical preparations. The proposed methods were based on electrochemical oxidation of bosentan at platinum electrode in acetonitrile solution containing 0.1 M TBACIO4. The well-defined oxidation peak was observed at 1.21 V. The calibration curves were linear for bosentan at the concentration range of 5-40 µg/mL for LSV and 5-35 µg/mL for SWV and DPV methods, respectively. Intra- and inter-day precision values for bosentan were less than 4.92, and accuracy (relative error) was better than 6.29%. The mean recovery of bosentan was 100.7% for pharmaceutical preparations. No interference was found from two tablet excipients at the selected assay conditions. Developed methods in this study are accurate, precise and can be easily applied to Tracleer and Diamond tablets as pharmaceutical preparation.

No MeSH data available.


Related in: MedlinePlus

a) Differential pulse voltammograms for different concentrations of bosentan in acetonitrile solution containing 0.1 M TBACIO4 (5, 10, 15, 20, 25, 30 and 35 μg/mL), b) Mean calibration graph (n=6)
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Figure 8: a) Differential pulse voltammograms for different concentrations of bosentan in acetonitrile solution containing 0.1 M TBACIO4 (5, 10, 15, 20, 25, 30 and 35 μg/mL), b) Mean calibration graph (n=6)

Mentions: Standard solutions were prepared as 5-40 μg/mL (5, 10, 15, 20, 30, 35 and 40) for LSV (Figure 6) and 5-35 μg/mL (5, 10, 15, 20, 25, 30 and 35) for SWV and DPV (Figures 7, 8), respectively. Calibration curves were constructed for bosentan standard by plotting the concentration of compound versus peak current responses. The calibration curves were evaluated by its correlation coefficients. The correlation coefficients (r) of all the calibration curves were consistently greater than 0.99. The linear regression equations were calculated by the least squares method using Microsoft Excel® program and summarized in Table 1.


Determination of bosentan in pharmaceutical preparations by linear sweep, square wave and differential pulse voltammetry methods.

Atila A, Yilmaz B - Iran J Pharm Res (2015)

a) Differential pulse voltammograms for different concentrations of bosentan in acetonitrile solution containing 0.1 M TBACIO4 (5, 10, 15, 20, 25, 30 and 35 μg/mL), b) Mean calibration graph (n=6)
© Copyright Policy
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4403060&req=5

Figure 8: a) Differential pulse voltammograms for different concentrations of bosentan in acetonitrile solution containing 0.1 M TBACIO4 (5, 10, 15, 20, 25, 30 and 35 μg/mL), b) Mean calibration graph (n=6)
Mentions: Standard solutions were prepared as 5-40 μg/mL (5, 10, 15, 20, 30, 35 and 40) for LSV (Figure 6) and 5-35 μg/mL (5, 10, 15, 20, 25, 30 and 35) for SWV and DPV (Figures 7, 8), respectively. Calibration curves were constructed for bosentan standard by plotting the concentration of compound versus peak current responses. The calibration curves were evaluated by its correlation coefficients. The correlation coefficients (r) of all the calibration curves were consistently greater than 0.99. The linear regression equations were calculated by the least squares method using Microsoft Excel® program and summarized in Table 1.

Bottom Line: Intra- and inter-day precision values for bosentan were less than 4.92, and accuracy (relative error) was better than 6.29%.The mean recovery of bosentan was 100.7% for pharmaceutical preparations.No interference was found from two tablet excipients at the selected assay conditions.

View Article: PubMed Central - PubMed

Affiliation: Department of Analytical Chemistry, Faculty of Pharmacy, Ataturk University, 25240, Erzurum, Turkey.

ABSTRACT
In this study, simple, fast and reliable cyclic voltammetry (CV), linear sweep voltammetry (LSV), square wave voltammetry (SWV) and differential pulse voltammetry (DPV) methods were developed and validated for determination of bosentan in pharmaceutical preparations. The proposed methods were based on electrochemical oxidation of bosentan at platinum electrode in acetonitrile solution containing 0.1 M TBACIO4. The well-defined oxidation peak was observed at 1.21 V. The calibration curves were linear for bosentan at the concentration range of 5-40 µg/mL for LSV and 5-35 µg/mL for SWV and DPV methods, respectively. Intra- and inter-day precision values for bosentan were less than 4.92, and accuracy (relative error) was better than 6.29%. The mean recovery of bosentan was 100.7% for pharmaceutical preparations. No interference was found from two tablet excipients at the selected assay conditions. Developed methods in this study are accurate, precise and can be easily applied to Tracleer and Diamond tablets as pharmaceutical preparation.

No MeSH data available.


Related in: MedlinePlus