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Determination of bosentan in pharmaceutical preparations by linear sweep, square wave and differential pulse voltammetry methods.

Atila A, Yilmaz B - Iran J Pharm Res (2015)

Bottom Line: Intra- and inter-day precision values for bosentan were less than 4.92, and accuracy (relative error) was better than 6.29%.The mean recovery of bosentan was 100.7% for pharmaceutical preparations.No interference was found from two tablet excipients at the selected assay conditions.

View Article: PubMed Central - PubMed

Affiliation: Department of Analytical Chemistry, Faculty of Pharmacy, Ataturk University, 25240, Erzurum, Turkey.

ABSTRACT
In this study, simple, fast and reliable cyclic voltammetry (CV), linear sweep voltammetry (LSV), square wave voltammetry (SWV) and differential pulse voltammetry (DPV) methods were developed and validated for determination of bosentan in pharmaceutical preparations. The proposed methods were based on electrochemical oxidation of bosentan at platinum electrode in acetonitrile solution containing 0.1 M TBACIO4. The well-defined oxidation peak was observed at 1.21 V. The calibration curves were linear for bosentan at the concentration range of 5-40 µg/mL for LSV and 5-35 µg/mL for SWV and DPV methods, respectively. Intra- and inter-day precision values for bosentan were less than 4.92, and accuracy (relative error) was better than 6.29%. The mean recovery of bosentan was 100.7% for pharmaceutical preparations. No interference was found from two tablet excipients at the selected assay conditions. Developed methods in this study are accurate, precise and can be easily applied to Tracleer and Diamond tablets as pharmaceutical preparation.

No MeSH data available.


Related in: MedlinePlus

Linear sweep voltammograms for the oxidation of 20 μg/mL bosentan in acetonitrile containing 0.1 M TBAClO4 as a function of scan rate.
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Figure 3: Linear sweep voltammograms for the oxidation of 20 μg/mL bosentan in acetonitrile containing 0.1 M TBAClO4 as a function of scan rate.

Mentions: In order to gain a deeper insight into the voltammetric waves, the effect of scan rate on the anodic peak currents (İm) and peak potentials (Ep) was studied in the range of 0.01-1 V/s of the potential scan rates in acetonitrile solution containing 20 μg/mL concentration of bosentan (Figure 3). The representative linear sweep voltammograms obtained at Pt electrode for 20 μg/mL bosentan as a function of the scan rate are presented in Figure 4. Scan rate dependency experiments show that the peak currents for peak vary linearly with the scan rate (ν) (Figure 4a,b), which points out the adsorption-controlled process. However, the plots of logarithm of peak currents versus logarithm of scan rates for 20 μg/mL concentration of bosentan display straight lines with 0.52 slope (Figure 4c), which are close to theoretical value of 0.5 expected for an ideal diffusion-controlled electrode process (18). Log Im-log ν curve is more eligible for this aim, therefore, a diffusional process for peak should be considered. These results suggest that the redox species are diffusing freely from solution and not precipitating onto the electrode surface. The reason for this behavior may be due to the solubility of the intermediate species in acetonitrile or poor adherence of products on the electrode surface.


Determination of bosentan in pharmaceutical preparations by linear sweep, square wave and differential pulse voltammetry methods.

Atila A, Yilmaz B - Iran J Pharm Res (2015)

Linear sweep voltammograms for the oxidation of 20 μg/mL bosentan in acetonitrile containing 0.1 M TBAClO4 as a function of scan rate.
© Copyright Policy
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4403060&req=5

Figure 3: Linear sweep voltammograms for the oxidation of 20 μg/mL bosentan in acetonitrile containing 0.1 M TBAClO4 as a function of scan rate.
Mentions: In order to gain a deeper insight into the voltammetric waves, the effect of scan rate on the anodic peak currents (İm) and peak potentials (Ep) was studied in the range of 0.01-1 V/s of the potential scan rates in acetonitrile solution containing 20 μg/mL concentration of bosentan (Figure 3). The representative linear sweep voltammograms obtained at Pt electrode for 20 μg/mL bosentan as a function of the scan rate are presented in Figure 4. Scan rate dependency experiments show that the peak currents for peak vary linearly with the scan rate (ν) (Figure 4a,b), which points out the adsorption-controlled process. However, the plots of logarithm of peak currents versus logarithm of scan rates for 20 μg/mL concentration of bosentan display straight lines with 0.52 slope (Figure 4c), which are close to theoretical value of 0.5 expected for an ideal diffusion-controlled electrode process (18). Log Im-log ν curve is more eligible for this aim, therefore, a diffusional process for peak should be considered. These results suggest that the redox species are diffusing freely from solution and not precipitating onto the electrode surface. The reason for this behavior may be due to the solubility of the intermediate species in acetonitrile or poor adherence of products on the electrode surface.

Bottom Line: Intra- and inter-day precision values for bosentan were less than 4.92, and accuracy (relative error) was better than 6.29%.The mean recovery of bosentan was 100.7% for pharmaceutical preparations.No interference was found from two tablet excipients at the selected assay conditions.

View Article: PubMed Central - PubMed

Affiliation: Department of Analytical Chemistry, Faculty of Pharmacy, Ataturk University, 25240, Erzurum, Turkey.

ABSTRACT
In this study, simple, fast and reliable cyclic voltammetry (CV), linear sweep voltammetry (LSV), square wave voltammetry (SWV) and differential pulse voltammetry (DPV) methods were developed and validated for determination of bosentan in pharmaceutical preparations. The proposed methods were based on electrochemical oxidation of bosentan at platinum electrode in acetonitrile solution containing 0.1 M TBACIO4. The well-defined oxidation peak was observed at 1.21 V. The calibration curves were linear for bosentan at the concentration range of 5-40 µg/mL for LSV and 5-35 µg/mL for SWV and DPV methods, respectively. Intra- and inter-day precision values for bosentan were less than 4.92, and accuracy (relative error) was better than 6.29%. The mean recovery of bosentan was 100.7% for pharmaceutical preparations. No interference was found from two tablet excipients at the selected assay conditions. Developed methods in this study are accurate, precise and can be easily applied to Tracleer and Diamond tablets as pharmaceutical preparation.

No MeSH data available.


Related in: MedlinePlus