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Comparison of Different Crosslinking Methods for Preparation of Docetaxel-loaded Albumin Nanoparticles.

Niknejad H, Mahmoudzadeh R - Iran J Pharm Res (2015)

Bottom Line: The results of FTIR assay were the same for all groups.Although crosslinking by UV or glucose alone resulted in cytotoxic effects, combination of UV and glucose had less cytotoxic effects compared to GA.Cellular uptake of nanoparticles crosslinked with UV + glucose and GA showed similar results.

View Article: PubMed Central - PubMed

Affiliation: Department of Tissue Engineering, School of Advanced Technologies in Medicine, Shahid Beheshti University of Medical Sciences, Tehran, Iran. ; Nanomedicine and Tissue Engineering Research Center, Shahid Beheshti University of Medical Sciences, Tehran, Iran.

ABSTRACT
In the last step of desolvation method for preparation of albumin nanoparticles, glutaraldehyde (GA) is added to stabilize the newly formed nanoparticles. Due to undesirable effects of GA, the objective of this study was to evaluate alternative methods of crosslinking including ultraviolet (UV) irradiation, adding of glucose and combination of both methods. The nanoparticles were prepared by desolvation procedure. Final particle size, zeta potential, FTIR, scanning electron micrograph, cellular uptake and cell toxicity of nanoparticles crosslinked with UV and/or glucose were compared with commonly crosslinked nanoparticles with GA. Moreover, drug release and stability parameters of docetaxel-loaded albumin nanoparticles were investigated. Size of all nanoparticles prepared by different methods was in the same range (100-200 nm). Zeta potential showed the same results except for those treated with UV. The results of FTIR assay were the same for all groups. Although crosslinking by UV or glucose alone resulted in cytotoxic effects, combination of UV and glucose had less cytotoxic effects compared to GA. Cellular uptake of nanoparticles crosslinked with UV + glucose and GA showed similar results. The release of docetaxel from UV + glucose and GA crosslinked nanoparticles showed the same biphasic release. These data support the idea that crosslinking with a combination of UV and glucose can be a promising alternative method for production of docetaxel-loaded albumin nanoparticles with the advantage of omitting toxic GA.

No MeSH data available.


Related in: MedlinePlus

The effect of storage time on particle size and polydispersity of docetaxel loaded albumin nanoparticles cosslinked with UV+Glucose
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Figure 6: The effect of storage time on particle size and polydispersity of docetaxel loaded albumin nanoparticles cosslinked with UV+Glucose

Mentions: To consider the storage stability, the docetaxel-loaded nanoparticles crosslinked with UV+Glucose were stored for up to 1.5 years in water at 4 ºC and at predefined times the samples were analyzed with regard to size and polydispersity. After 7 and 21 days of storage, the particles showed a uniform sedimentation (Figure 6). However, the particles were easily re-dispersed by shaking for about 1 min. After 21 days, the particle size slightly decreased (from 330 nm to 274 nm). After 6 months, the nanoparticles still showed an acceptable size 380 nm. There is no significant change in polydispersity after 6 months. After storage periods of 1 and 1.5 years particles became difficult to re-disperse and sizes increased into about the micrometer range with a multimodal size distribution. Therefore, long-term storage of the particles in suspension is not possible.


Comparison of Different Crosslinking Methods for Preparation of Docetaxel-loaded Albumin Nanoparticles.

Niknejad H, Mahmoudzadeh R - Iran J Pharm Res (2015)

The effect of storage time on particle size and polydispersity of docetaxel loaded albumin nanoparticles cosslinked with UV+Glucose
© Copyright Policy
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4403054&req=5

Figure 6: The effect of storage time on particle size and polydispersity of docetaxel loaded albumin nanoparticles cosslinked with UV+Glucose
Mentions: To consider the storage stability, the docetaxel-loaded nanoparticles crosslinked with UV+Glucose were stored for up to 1.5 years in water at 4 ºC and at predefined times the samples were analyzed with regard to size and polydispersity. After 7 and 21 days of storage, the particles showed a uniform sedimentation (Figure 6). However, the particles were easily re-dispersed by shaking for about 1 min. After 21 days, the particle size slightly decreased (from 330 nm to 274 nm). After 6 months, the nanoparticles still showed an acceptable size 380 nm. There is no significant change in polydispersity after 6 months. After storage periods of 1 and 1.5 years particles became difficult to re-disperse and sizes increased into about the micrometer range with a multimodal size distribution. Therefore, long-term storage of the particles in suspension is not possible.

Bottom Line: The results of FTIR assay were the same for all groups.Although crosslinking by UV or glucose alone resulted in cytotoxic effects, combination of UV and glucose had less cytotoxic effects compared to GA.Cellular uptake of nanoparticles crosslinked with UV + glucose and GA showed similar results.

View Article: PubMed Central - PubMed

Affiliation: Department of Tissue Engineering, School of Advanced Technologies in Medicine, Shahid Beheshti University of Medical Sciences, Tehran, Iran. ; Nanomedicine and Tissue Engineering Research Center, Shahid Beheshti University of Medical Sciences, Tehran, Iran.

ABSTRACT
In the last step of desolvation method for preparation of albumin nanoparticles, glutaraldehyde (GA) is added to stabilize the newly formed nanoparticles. Due to undesirable effects of GA, the objective of this study was to evaluate alternative methods of crosslinking including ultraviolet (UV) irradiation, adding of glucose and combination of both methods. The nanoparticles were prepared by desolvation procedure. Final particle size, zeta potential, FTIR, scanning electron micrograph, cellular uptake and cell toxicity of nanoparticles crosslinked with UV and/or glucose were compared with commonly crosslinked nanoparticles with GA. Moreover, drug release and stability parameters of docetaxel-loaded albumin nanoparticles were investigated. Size of all nanoparticles prepared by different methods was in the same range (100-200 nm). Zeta potential showed the same results except for those treated with UV. The results of FTIR assay were the same for all groups. Although crosslinking by UV or glucose alone resulted in cytotoxic effects, combination of UV and glucose had less cytotoxic effects compared to GA. Cellular uptake of nanoparticles crosslinked with UV + glucose and GA showed similar results. The release of docetaxel from UV + glucose and GA crosslinked nanoparticles showed the same biphasic release. These data support the idea that crosslinking with a combination of UV and glucose can be a promising alternative method for production of docetaxel-loaded albumin nanoparticles with the advantage of omitting toxic GA.

No MeSH data available.


Related in: MedlinePlus