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Preparation and In-vitro Evaluation of Rifampin-loaded Mesoporous Silica Nanoaggregates by an Experimental Design.

Mohseni M, Gilani K, Bahrami Z, Bolourchian N, Mortazavi SA - Iran J Pharm Res (2015)

Bottom Line: The results showed that feed concentration, feed pH and the interaction between feed flow rate and gas atomizer flow rate had statistically significant effects on the particle size of nanoaggregates.The rifampin-loaded silica nanoaggregates were capable of releasing 90% drug content after 24 h in combination patterns of release.The prepared rifampin-loaded nanoaggregates seem to have a potential to be used in a pulmonary drug delivery.

View Article: PubMed Central - PubMed

Affiliation: Department of Pharmaceutics, School of Pharmacy, Shahid Beheshti University of Medical Sciences, Tehran, Iran.

ABSTRACT
The goal of this research is preparation, optimization and in-vitro evaluation of rifampin-loaded silica nanoparticles in order to use in the pulmonary drug delivery. Nanoparticles are exhaled because of their small size. Preparation of nanoaggregates in a micron-size scale and re-dispersion of them after deposition in the lung is an approach to overcome this problem. We used this approach in our research. Rifampin was selected as a model lipophilic molecule since it was a well-documented and much used anti tuberculosis drug. A half factorial design was used to identify significant parameters of the spray drying process. The results showed that feed concentration, feed pH and the interaction between feed flow rate and gas atomizer flow rate had statistically significant effects on the particle size of nanoaggregates. The Box-Behnken design was employed to optimize the spray drying process. Finally, a quadratic equation which explains the relation between independent variables and aerodynamic diameter of nanoaggregates was obtained. Rifampin-loaded silica nanoaggregates underwent different in-vitro tests including: SEM, Aerosol performance and drug release. The in-vitro drug release was investigated with buffer phosphate (pH=7.4). Regarding the drug release study, a triphasic pattern of release was observed. The rifampin-loaded silica nanoaggregates were capable of releasing 90% drug content after 24 h in combination patterns of release. The prepared rifampin-loaded nanoaggregates seem to have a potential to be used in a pulmonary drug delivery.

No MeSH data available.


Related in: MedlinePlus

Pareto chart of dA at 95% confidence interval. A, B, C, D and E are concentration, pH, temperature, feed rate and gas atomizing flow rate respectively.
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Figure 1: Pareto chart of dA at 95% confidence interval. A, B, C, D and E are concentration, pH, temperature, feed rate and gas atomizing flow rate respectively.

Mentions: The Pareto chart of the main and interaction effects is shown in Figure 1. The Pareto chart is used to identify experimental parameters that have a statistically significant influence on a particular response. The Pareto chart displays the magnitude of the effects and draws a reference line at a 95% confidence level. Effects with a magnitude that extends beyond the reference line are statistically significant(43). The Pareto chart for dA reveals that the significant spray drying formulation parameters are the feed concentration, the feed pH, and the interaction between the feed rate and the gas atomizing flow rate. The inlet temperature, which is known to significantly influence dA of spray dried particles, is found to have a reduced impact on the nanoaggregate production. The effect of the inlet temperature selection on dA is insignificant provided that the selected value can adequately provide a drying rate to produce the nanoaggregates.


Preparation and In-vitro Evaluation of Rifampin-loaded Mesoporous Silica Nanoaggregates by an Experimental Design.

Mohseni M, Gilani K, Bahrami Z, Bolourchian N, Mortazavi SA - Iran J Pharm Res (2015)

Pareto chart of dA at 95% confidence interval. A, B, C, D and E are concentration, pH, temperature, feed rate and gas atomizing flow rate respectively.
© Copyright Policy
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4403052&req=5

Figure 1: Pareto chart of dA at 95% confidence interval. A, B, C, D and E are concentration, pH, temperature, feed rate and gas atomizing flow rate respectively.
Mentions: The Pareto chart of the main and interaction effects is shown in Figure 1. The Pareto chart is used to identify experimental parameters that have a statistically significant influence on a particular response. The Pareto chart displays the magnitude of the effects and draws a reference line at a 95% confidence level. Effects with a magnitude that extends beyond the reference line are statistically significant(43). The Pareto chart for dA reveals that the significant spray drying formulation parameters are the feed concentration, the feed pH, and the interaction between the feed rate and the gas atomizing flow rate. The inlet temperature, which is known to significantly influence dA of spray dried particles, is found to have a reduced impact on the nanoaggregate production. The effect of the inlet temperature selection on dA is insignificant provided that the selected value can adequately provide a drying rate to produce the nanoaggregates.

Bottom Line: The results showed that feed concentration, feed pH and the interaction between feed flow rate and gas atomizer flow rate had statistically significant effects on the particle size of nanoaggregates.The rifampin-loaded silica nanoaggregates were capable of releasing 90% drug content after 24 h in combination patterns of release.The prepared rifampin-loaded nanoaggregates seem to have a potential to be used in a pulmonary drug delivery.

View Article: PubMed Central - PubMed

Affiliation: Department of Pharmaceutics, School of Pharmacy, Shahid Beheshti University of Medical Sciences, Tehran, Iran.

ABSTRACT
The goal of this research is preparation, optimization and in-vitro evaluation of rifampin-loaded silica nanoparticles in order to use in the pulmonary drug delivery. Nanoparticles are exhaled because of their small size. Preparation of nanoaggregates in a micron-size scale and re-dispersion of them after deposition in the lung is an approach to overcome this problem. We used this approach in our research. Rifampin was selected as a model lipophilic molecule since it was a well-documented and much used anti tuberculosis drug. A half factorial design was used to identify significant parameters of the spray drying process. The results showed that feed concentration, feed pH and the interaction between feed flow rate and gas atomizer flow rate had statistically significant effects on the particle size of nanoaggregates. The Box-Behnken design was employed to optimize the spray drying process. Finally, a quadratic equation which explains the relation between independent variables and aerodynamic diameter of nanoaggregates was obtained. Rifampin-loaded silica nanoaggregates underwent different in-vitro tests including: SEM, Aerosol performance and drug release. The in-vitro drug release was investigated with buffer phosphate (pH=7.4). Regarding the drug release study, a triphasic pattern of release was observed. The rifampin-loaded silica nanoaggregates were capable of releasing 90% drug content after 24 h in combination patterns of release. The prepared rifampin-loaded nanoaggregates seem to have a potential to be used in a pulmonary drug delivery.

No MeSH data available.


Related in: MedlinePlus