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Phyto-extraction of heavy metals and biochemical changes with Brassica nigra L. grown in rayon grade paper mill effluent irrigated soil.

Singh UV, Abhishek A, Bhaskar M, Tandan N, Ansari NG, Singh NP - Bioinformation (2015)

Bottom Line: An increase in the chlorophyll and protein contents was also recorded at <50% (v/v) RGPE followed by a decrease at higher concentrations of RGPE (>75%).This Indian mustard plants (Brassica nigra L.) are well adapted for tolerance of significant amount of heavy metals due to increased level of antioxidants (cysteine and ascorbic acid) in root shoot and leaves of treated plants at all concentration of RGPE.Moreover, it is also important that RGPE should be treated to bring down the metal concentration well within the prescribed limit prior to use in agricultural soil for ferti-irrigation.

View Article: PubMed Central - PubMed

Affiliation: Environmental Chemistry Division, CSIR-Indian Institute of Toxicology Research Centre, Post Box 80, M.G. Marg, Lucknow-226001, U. P., INDIA.

ABSTRACT
In this study, distribution of metal accumulation and their biological changes of Indian mustard plants (Brassica nigra L.) grown in soil irrigated with different concentration of rayon grade paper effluent (RGPE, 25%, 50%, 75%, 100%, v/v) were studied. A pronounced effect was recorded at 50% (v/v) RGPE on germination of seeds, amylase activity and other growth parameters in Indian mustard plants. An increase in the chlorophyll and protein contents was also recorded at <50% (v/v) RGPE followed by a decrease at higher concentrations of RGPE (>75%). A significant increase lipid peroxidation was recorded, which was evidenced by the increased malondialdehyde (MDA) content in shoot, leaves and seeds in tested plant at all the concentrations of RGPE. This Indian mustard plants (Brassica nigra L.) are well adapted for tolerance of significant amount of heavy metals due to increased level of antioxidants (cysteine and ascorbic acid) in root shoot and leaves of treated plants at all concentration of RGPE. Moreover, it is also important that RGPE should be treated to bring down the metal concentration well within the prescribed limit prior to use in agricultural soil for ferti-irrigation.

No MeSH data available.


Chlorophyll-a, b, total chlorophyll and carotenoidcontent (mg g-1) in leaves (A), Protein content (mg g-1) (B),MDA content (µmol g-1) (C), of Indian mustard plants irrigatedwith RGPE and tap water (control).
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Related In: Results  -  Collection


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Figure 1: Chlorophyll-a, b, total chlorophyll and carotenoidcontent (mg g-1) in leaves (A), Protein content (mg g-1) (B),MDA content (µmol g-1) (C), of Indian mustard plants irrigatedwith RGPE and tap water (control).

Mentions: The increases chlorophyll contents (chlorophyll a, b and totalchlorophyll) in leaves of tested plant were recorded at allconcentrations of RGPE (25, 50, 75 and 100% v/v) at 30 and 60days of growth period. But, these pigments decreasedsignificantly beyond 50% RGPE at 60 and 90 days of growthperiod compared to their respective controls (Figure 1A). Themaximum increase of 44.7%, 40.6% and 47.0% were recorded inchlo-a, b and total chlorophyll content respectively in mustardplants treated with 50% RGPE at 60 days of growth period ascompared to their respective controls. The protein content inroot, shoot and leaves also increased compare to respectivecontrols in mustard plants irrigated with 50% (v/v) RGPE at 60days of growth period (Figure 1B). Nevertheless at higherconcentrations (>50%, v/v) of RGPE, there was a decrease inprotein content of root, shoot and leaves at 60 and 90 days ofgrowth period. Moreover, the MDA content in root and leavesof mustard plants treated with RGPE increased with rise inRGPE content versus to control at all the exposure periodsindicating the enhanced lipid peroxidation in mustard plantsgrown in RGPE irrigated soil Figure 1C.


Phyto-extraction of heavy metals and biochemical changes with Brassica nigra L. grown in rayon grade paper mill effluent irrigated soil.

Singh UV, Abhishek A, Bhaskar M, Tandan N, Ansari NG, Singh NP - Bioinformation (2015)

Chlorophyll-a, b, total chlorophyll and carotenoidcontent (mg g-1) in leaves (A), Protein content (mg g-1) (B),MDA content (µmol g-1) (C), of Indian mustard plants irrigatedwith RGPE and tap water (control).
© Copyright Policy - open-access
Related In: Results  -  Collection

Show All Figures
getmorefigures.php?uid=PMC4403035&req=5

Figure 1: Chlorophyll-a, b, total chlorophyll and carotenoidcontent (mg g-1) in leaves (A), Protein content (mg g-1) (B),MDA content (µmol g-1) (C), of Indian mustard plants irrigatedwith RGPE and tap water (control).
Mentions: The increases chlorophyll contents (chlorophyll a, b and totalchlorophyll) in leaves of tested plant were recorded at allconcentrations of RGPE (25, 50, 75 and 100% v/v) at 30 and 60days of growth period. But, these pigments decreasedsignificantly beyond 50% RGPE at 60 and 90 days of growthperiod compared to their respective controls (Figure 1A). Themaximum increase of 44.7%, 40.6% and 47.0% were recorded inchlo-a, b and total chlorophyll content respectively in mustardplants treated with 50% RGPE at 60 days of growth period ascompared to their respective controls. The protein content inroot, shoot and leaves also increased compare to respectivecontrols in mustard plants irrigated with 50% (v/v) RGPE at 60days of growth period (Figure 1B). Nevertheless at higherconcentrations (>50%, v/v) of RGPE, there was a decrease inprotein content of root, shoot and leaves at 60 and 90 days ofgrowth period. Moreover, the MDA content in root and leavesof mustard plants treated with RGPE increased with rise inRGPE content versus to control at all the exposure periodsindicating the enhanced lipid peroxidation in mustard plantsgrown in RGPE irrigated soil Figure 1C.

Bottom Line: An increase in the chlorophyll and protein contents was also recorded at <50% (v/v) RGPE followed by a decrease at higher concentrations of RGPE (>75%).This Indian mustard plants (Brassica nigra L.) are well adapted for tolerance of significant amount of heavy metals due to increased level of antioxidants (cysteine and ascorbic acid) in root shoot and leaves of treated plants at all concentration of RGPE.Moreover, it is also important that RGPE should be treated to bring down the metal concentration well within the prescribed limit prior to use in agricultural soil for ferti-irrigation.

View Article: PubMed Central - PubMed

Affiliation: Environmental Chemistry Division, CSIR-Indian Institute of Toxicology Research Centre, Post Box 80, M.G. Marg, Lucknow-226001, U. P., INDIA.

ABSTRACT
In this study, distribution of metal accumulation and their biological changes of Indian mustard plants (Brassica nigra L.) grown in soil irrigated with different concentration of rayon grade paper effluent (RGPE, 25%, 50%, 75%, 100%, v/v) were studied. A pronounced effect was recorded at 50% (v/v) RGPE on germination of seeds, amylase activity and other growth parameters in Indian mustard plants. An increase in the chlorophyll and protein contents was also recorded at <50% (v/v) RGPE followed by a decrease at higher concentrations of RGPE (>75%). A significant increase lipid peroxidation was recorded, which was evidenced by the increased malondialdehyde (MDA) content in shoot, leaves and seeds in tested plant at all the concentrations of RGPE. This Indian mustard plants (Brassica nigra L.) are well adapted for tolerance of significant amount of heavy metals due to increased level of antioxidants (cysteine and ascorbic acid) in root shoot and leaves of treated plants at all concentration of RGPE. Moreover, it is also important that RGPE should be treated to bring down the metal concentration well within the prescribed limit prior to use in agricultural soil for ferti-irrigation.

No MeSH data available.